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A7IAA9 (DNLI_METB6) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA ligase

EC=6.5.1.1
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP]
Gene names
Name:lig
Ordered Locus Names:Mboo_2156
OrganismMethanoregula boonei (strain 6A8) [Complete proteome] [HAMAP]
Taxonomic identifier456442 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanoregulaceaeMethanoregula

Protein attributes

Sequence length550 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair By similarity. HAMAP-Rule MF_00407

Catalytic activity

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m). HAMAP-Rule MF_00407

Cofactor

Divalent metal cations By similarity. HAMAP-Rule MF_00407

Sequence similarities

Belongs to the ATP-dependent DNA ligase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 550550DNA ligase HAMAP-Rule MF_00407
PRO_0000365256

Sites

Active site2461N6-AMP-lysine intermediate By similarity
Binding site2441ATP By similarity
Binding site2511ATP By similarity
Binding site2661ATP By similarity
Binding site2951ATP By similarity
Binding site3341ATP By similarity
Binding site4051ATP By similarity
Binding site4111ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A7IAA9 [UniParc].

Last modified September 11, 2007. Version 1.
Checksum: 9029C3918A446474

FASTA55061,583
        10         20         30         40         50         60 
MLFSEFAQVC EELEHLSGRL DMIEVISRAL PDLAPDELPV FVRFVMGRIF PDWSAQKLGI 

        70         80         90        100        110        120 
GPNLLYEAVR QAAGVKLETV ITRINQQGDV GRAVEDILAK KTQVSWSHQD LELVYVYNKL 

       130        140        150        160        170        180 
TGISSRGGVT SQKEKIRIAM LLLGDASPLE GRYLARIMLE ELRIGVGEGT VREAIAKAFM 

       190        200        210        220        230        240 
VDSALVEHAM QAINDLGEVA RLAKKGPTAL SDVHITPFHP VKMMLAQQGT IAGMIEDHGE 

       250        260        270        280        290        300 
IAAEYKYDGS RFQFHKEGAK ARMYSRRLED VSEALPDVID LLSKATSHDV ILDGEVIAIK 

       310        320        330        340        350        360 
DDRPMPFQSV LRRFRRRHDI AEAQEAIRMV PNVFDILYLD GETLIDLPFF ERRKKLETVV 

       370        380        390        400        410        420 
GKFVAPQVVS TDPQTIEQTY HDALAAGHEG IMLKVPASPY TPGQRGKNWI KIKPEVDTLD 

       430        440        450        460        470        480 
LAVIGAEWGE GKRAHVFGSF LVACQDQGKL IPLSRVATGF SDEQLTEVYD LLKDAVISRT 

       490        500        510        520        530        540 
GKEVRFEPEL VFEVGYAELQ VSPTYDAGFA LRFPRFIRIR DDKDTTEIET LESIRGRYQR 

       550 
QAKSAQAYTK 

« Hide

References

[1]"Complete sequence of Candidatus Methanoregula boonei 6A8."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Meincke L., Brettin T., Bruce D., Detter J.C., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Kim E., Zinder S., Richardson P.
Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 6A8.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000780 Genomic DNA. Translation: ABS56670.1.
RefSeqYP_001405313.1. NC_009712.1.

3D structure databases

ProteinModelPortalA7IAA9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING456442.Mboo_2156.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABS56670; ABS56670; Mboo_2156.
GeneID5410132.
KEGGmbn:Mboo_2156.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1793.
HOGENOMHOG000036008.
KOK10747.
OMAGRPRPFQ.
ProtClustDBCLSK949919.

Enzyme and pathway databases

BioCycMBOO456442:GH2T-2201-MONOMER.

Family and domain databases

Gene3D1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPMF_00407. DNA_ligase.
InterProIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsTIGR00574. dnl1. 1 hit.
PROSITEPS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDNLI_METB6
AccessionPrimary (citable) accession number: A7IAA9
Entry history
Integrated into UniProtKB/Swiss-Prot: March 3, 2009
Last sequence update: September 11, 2007
Last modified: February 19, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families