Reviewed, UniProtKB/Swiss-Prot A7IA92 (GUAAB_METB6)

Names and origin

Protein names

Recommended name:
    GMP synthase [glutamine-hydrolyzing] subunit B
    EC=6.3.5.2
Alternative name(s):
    GMP synthetase

Gene names

Name:	guaAB
Ordered Locus Names:	Mboo_2139

Organism

Methanoregula boonei (strain 6A8) [Complete proteome] [HAMAP]

Taxonomic identifier

456442 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanomicrobiales › Genera incertae sedis › Candidatus Methanoregula

Protein attributes

Sequence length	305 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the synthesis of GMP from XMP By similarity. HAMAP MF_00345
Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_00345
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_00345
Subunit structure	Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B) Potential. HAMAP MF_00345
Sequence similarities	Contains 1 GMP-binding domain.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: HAMAP NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 305	305	GMP synthase [glutamine-hydrolyzing] subunit B HAMAP MF_00345		PRO_1000048375
Regions
	Domain	33 – 185	153	GMP-binding
	Nucleotide binding	29 – 35	7	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7IA92-1 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: C1DF31A53C83A2D7	FASTA	305	33,434
	10 20 30 40 50 60 MVKTEKFIQK SVEEIQKEAG NEKVVMALSG GVDSSVCASL AARAIGDRLI PIYIDTGLMR 70 80 90 100 110 120 KGETERIKAV FGNIRLQVVD AGDEFVAALA GITDPEKKRK AIGERFIRVF EREAKKSGAT 130 140 150 160 170 180 CLLQGTIYPD RIESEGGIKS HHNVGGMPEH TAFKKVIEPI RDLYKDEVRE VAGALGLPPE 190 200 210 220 230 240 IQHRMPFPGP GLAVRILGEV TKEKVAVIRE ANWIAESEIV EKYRPWQCFA ALIGLGTGVK 250 260 270 280 290 300 GDNRIHGWIV AVRAVNSRDG MTADPLEIPF ADLVRIGSRI TAEIPSVARV VYDITPKPPA TIEYE

References

[1]

"Complete sequence of Candidatus Methanoregula boonei 6A8." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Meincke L., Brettin T., Bruce D., Detter J.C., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Zinder S., Richardson P. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000780 Genomic DNA. Translation: ABS56653.1.
RefSeq	YP_001405296.1.
3D structure databases
SMR	A7IA92. Positions 4-305.
ModBase	Search...
Protein-protein interaction databases
STRING	A7IA92.
Genome annotation databases
GeneID	5409957.
GenomeReviews	Gene locus Mboo_2139 in contig CP000780_GR.
KEGG	mbn:Mboo_2139.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04539.
HOGENOM	HBG391775.
OMA	GIKSHHN.
Family and domain databases
HAMAP	MF_00345. GMP_synthase_B. [Tree]
InterPro	IPR001674. GMP_synth_C. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
Pfam	PF00958. GMP_synt_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00884. guaA_Cterm. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GUAAB_METB6

Accession

Primary (citable) accession number: A7IA92

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 11, 2007
Last modified:	February 9, 2010
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)