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Protein

Quinolinate synthase A

Gene

nadA

Organism
Methanoregula boonei (strain 6A8)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the condensation of iminoaspartate with dihydroxyacetone phosphate to form quinolinate.UniRule annotation

Catalytic activityi

Glycerone phosphate + iminosuccinate = pyridine-2,3-dicarboxylate + 2 H2O + phosphate.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per subunit.UniRule annotation

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes quinolinate from iminoaspartate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Quinolinate synthase A (nadA)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes quinolinate from iminoaspartate, the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei25 – 251IminoaspartateUniRule annotation
Binding sitei42 – 421Iminoaspartate; via amide nitrogenUniRule annotation
Binding sitei113 – 1131IminoaspartateUniRule annotation
Binding sitei130 – 1301IminoaspartateUniRule annotation
Binding sitei198 – 1981IminoaspartateUniRule annotation
Binding sitei215 – 2151IminoaspartateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Pyridine nucleotide biosynthesis

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciMBOO456442:GH2T-2164-MONOMER.
UniPathwayiUPA00253; UER00327.

Names & Taxonomyi

Protein namesi
Recommended name:
Quinolinate synthase AUniRule annotation (EC:2.5.1.72UniRule annotation)
Gene namesi
Name:nadAUniRule annotation
Ordered Locus Names:Mboo_2119
OrganismiMethanoregula boonei (strain 6A8)
Taxonomic identifieri456442 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanoregulaceaeMethanoregula
Proteomesi
  • UP000002408 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 302302Quinolinate synthase APRO_1000146814Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi456442.Mboo_2119.

Structurei

3D structure databases

ProteinModelPortaliA7IA72.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the quinolinate synthase A family. Type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04459. Archaea.
COG0379. LUCA.
HOGENOMiHOG000222770.
KOiK03517.
OMAiDKCLGEN.

Family and domain databases

HAMAPiMF_00568. NadA_type2.
InterProiIPR003473. NadA.
IPR023066. Quinolinate_synth_type2.
[Graphical view]
PfamiPF02445. NadA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00550. nadA. 1 hit.

Sequencei

Sequence statusi: Complete.

A7IA72-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDDTALLQEE ILRLKEKKNA ILLAHNYQRP EVQDIADLTG DSLELARAAA
60 70 80 90 100
AMQGDTIVFC GVDFMAETAA ILAPDKTVLL PAADACCPMA EMVTAEELRF
110 120 130 140 150
ARARHPGAAV VCYVNTTAGV KAESDICCTS ANAISVVNSL TEDRVIFVPD
160 170 180 190 200
KNLGRYAARF TEKKVLPWEG FCLVHDRYTP DDVARAKDAH PGAAVVVHPE
210 220 230 240 250
CRPEVIDLAD HVASTSGIIR YVQTSPGSEF IIGTEIGILH RLSKECPGKR
260 270 280 290 300
CFPLSDKAVC VNMKKTTLVH VRDALVTGQP RITVPDEIAS RARRAIGRML

AL
Length:302
Mass (Da):32,791
Last modified:September 11, 2007 - v1
Checksum:i63FA9D763B4F574F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000780 Genomic DNA. Translation: ABS56633.1.
RefSeqiWP_012107691.1. NC_009712.1.

Genome annotation databases

EnsemblBacteriaiABS56633; ABS56633; Mboo_2119.
GeneIDi5411217.
KEGGimbn:Mboo_2119.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000780 Genomic DNA. Translation: ABS56633.1.
RefSeqiWP_012107691.1. NC_009712.1.

3D structure databases

ProteinModelPortaliA7IA72.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi456442.Mboo_2119.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABS56633; ABS56633; Mboo_2119.
GeneIDi5411217.
KEGGimbn:Mboo_2119.

Phylogenomic databases

eggNOGiarCOG04459. Archaea.
COG0379. LUCA.
HOGENOMiHOG000222770.
KOiK03517.
OMAiDKCLGEN.

Enzyme and pathway databases

UniPathwayiUPA00253; UER00327.
BioCyciMBOO456442:GH2T-2164-MONOMER.

Family and domain databases

HAMAPiMF_00568. NadA_type2.
InterProiIPR003473. NadA.
IPR023066. Quinolinate_synth_type2.
[Graphical view]
PfamiPF02445. NadA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00550. nadA. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 6A8.

Entry informationi

Entry nameiNADA_METB6
AccessioniPrimary (citable) accession number: A7IA72
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: September 11, 2007
Last modified: December 9, 2015
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.