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Protein

Hydroxyethylthiazole kinase

Gene

thiM

Organism
Methanoregula boonei (strain DSM 21154 / JCM 14090 / 6A8)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of the hydroxyl group of 4-methyl-5-beta-hydroxyethylthiazole (THZ).UniRule annotation

Catalytic activityi

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Hydroxyethylthiazole kinase (thiM)
This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei45Substrate; via amide nitrogenUniRule annotation1
Binding sitei121ATPUniRule annotation1
Binding sitei167ATPUniRule annotation1
Binding sitei194Substrate; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00060; UER00139.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyethylthiazole kinaseUniRule annotation (EC:2.7.1.50UniRule annotation)
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinaseUniRule annotation
Short name:
TH kinaseUniRule annotation
Short name:
Thz kinaseUniRule annotation
Gene namesi
Name:thiMUniRule annotation
Ordered Locus Names:Mboo_2055
OrganismiMethanoregula boonei (strain DSM 21154 / JCM 14090 / 6A8)
Taxonomic identifieri456442 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanoregulaceaeMethanoregula
Proteomesi
  • UP000002408 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000215191 – 266Hydroxyethylthiazole kinaseAdd BLAST266

Interactioni

Protein-protein interaction databases

STRINGi456442.Mboo_2055.

Structurei

3D structure databases

ProteinModelPortaliA7IA08.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Thz kinase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00019. Archaea.
COG2145. LUCA.
HOGENOMiHOG000114352.
KOiK00878.
OMAiSPVMAHA.

Family and domain databases

CDDicd01170. THZ_kinase. 1 hit.
Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00228. Thz_kinase. 1 hit.
InterProiIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF02110. HK. 1 hit.
[Graphical view]
PIRSFiPIRSF000513. Thz_kinase. 1 hit.
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00694. thiM. 1 hit.

Sequencei

Sequence statusi: Complete.

A7IA08-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDNQVFSGIF ARAREQHPLV HHITNYVTVN DCANITIGAG GAPVMADARE
60 70 80 90 100
EAGEMTGFAG ALVLNIGTLN SGIIESMILA GKVANERKIP IILDPVGAGA
110 120 130 140 150
TRLRTDSTRR LLDELTISII KGNAGEIGVL AGAEAKVRGV DSAGISGDPV
160 170 180 190 200
TITRTFARET GITVVMSGAT DIISDGNKVL LVDNGHPLMG RISGTGCMAS
210 220 230 240 250
AVTGVIAAVE KDRLIAAATA LAAFGLAGER AAAASRGPGS FKPALFDAMA
260
ELGPQDLAAD ARVRSA
Length:266
Mass (Da):27,142
Last modified:September 11, 2007 - v1
Checksum:i075B6F87F7D05204
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000780 Genomic DNA. Translation: ABS56569.1.
RefSeqiWP_012107625.1. NC_009712.1.

Genome annotation databases

EnsemblBacteriaiABS56569; ABS56569; Mboo_2055.
GeneIDi5410688.
KEGGimbn:Mboo_2055.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000780 Genomic DNA. Translation: ABS56569.1.
RefSeqiWP_012107625.1. NC_009712.1.

3D structure databases

ProteinModelPortaliA7IA08.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi456442.Mboo_2055.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABS56569; ABS56569; Mboo_2055.
GeneIDi5410688.
KEGGimbn:Mboo_2055.

Phylogenomic databases

eggNOGiarCOG00019. Archaea.
COG2145. LUCA.
HOGENOMiHOG000114352.
KOiK00878.
OMAiSPVMAHA.

Enzyme and pathway databases

UniPathwayiUPA00060; UER00139.

Family and domain databases

CDDicd01170. THZ_kinase. 1 hit.
Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00228. Thz_kinase. 1 hit.
InterProiIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF02110. HK. 1 hit.
[Graphical view]
PIRSFiPIRSF000513. Thz_kinase. 1 hit.
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00694. thiM. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTHIM_METB6
AccessioniPrimary (citable) accession number: A7IA08
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: September 11, 2007
Last modified: November 2, 2016
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.