A7I9R7 (SYP_METB6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proline--tRNA ligase
EC=6.1.1.15

Alternative name(s):
Prolyl-tRNA synthetase
Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	Mboo_1963

Organism

Methanoregula boonei (strain 6A8) [Complete proteome] [HAMAP]

Taxonomic identifier

456442 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanomicrobiales › Genera incertae sedis › Methanoregula

Protein attributes

Sequence length	478 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro) By similarity. HAMAP MF_01571
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01571
Subunit structure	Homodimer By similarity. HAMAP MF_01571
Subcellular location	Cytoplasm By similarity HAMAP MF_01571.
Domain	Consists of three domains: the N-terminal catalytic domain, the anticodon-binding domain and the C-terminal extension By similarity. HAMAP MF_01571
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW proline-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 478	478	Proline--tRNA ligase HAMAP MF_01571		PRO_1000069192

Sequences

	Sequence		Length	Mass (Da)
	A7I9R7 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: 3F0124FA09C73E64	FASTA	478	54,244
	10 20 30 40 50 60 METDTGALPK KQDFSEWYNE ILWRAEIMDV RYPVKGLYVW FPYGFAIRKF VYQHLRELLD 70 80 90 100 110 120 RDHKETLFPL LIPEQEFMKE AEHIKGFEDE VYWVTHGGTT PLEVKLALRP TSETAIYPMF 130 140 150 160 170 180 ALWVRSHADL PIKIYQIVNT FRYETKQTRP LIRLREITSF MESHTVHATW DEAEIQVESE 190 200 210 220 230 240 IALTREFYRN LCIPIIISKR PDWDKFPGAD YTIAVDAIMP NGKTLQIGTV HHLGNHFSRT 250 260 270 280 290 300 FNIQYEDKNG EQKEAYQTCY GISERCIAAL ISLHGDDKGL ILPPTVATFQ VVIVPITIGK 310 320 330 340 350 360 RHEDVLAAAG KLKNDLENAG LRVTLDTRDL RPGAKYYWWE IRGVPLRLEL GPRDLDSGKA 370 380 390 400 410 420 MAVTRTGEKT TICLANAAED TTSILTGITD AIRAKAGEHT KSHLCTTHTM DGLDIALNEG 430 440 450 460 470 KVAVVHWCRD RTCGDTIEEK ANSSLLGTEV RSEYIEATDG PCIICGKPGK ATLVGRTY

References

[1]

"Complete sequence of Candidatus Methanoregula boonei 6A8." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Meincke L., Brettin T., Bruce D., Detter J.C., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Zinder S., Richardson P. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 6A8.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000780 Genomic DNA. Translation: ABS56478.1.
RefSeq	YP_001405121.1. NC_009712.1.
3D structure databases
ProteinModelPortal	A7I9R7.
SMR	A7I9R7. Positions 13-478.
ModBase	Search...
Protein-protein interaction databases
STRING	A7I9R7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5410000.
GenomeReviews	Gene locus Mboo_1963 in contig CP000780_GR.
KEGG	mbn:Mboo_1963.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04466.
HOGENOM	HBG334108.
OMA	KFAEYEL.
ProtClustDB	PRK08661.
Enzyme and pathway databases
BioCyc	CMET456442:MBOO_1963-MONOMER.
Family and domain databases
HAMAP	MF_01571. Pro_tRNA_synth_type3. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR002316. Pro-tRNA-synth_IIa. IPR004499. Pro-tRNA-synth_IIa_arc-type. IPR017449. Pro-tRNA_synth_II. IPR016061. Pro-tRNA_synth_II_C. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.30.110.30. Pro-tRNA-synth_II_C_arc/euk. 1 hit.
KO	K01881.
PANTHER	PTHR11451:SF6. ProS_fam_I. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF09180. ProRS-C_1. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
SMART	SM00946. ProRS-C_1. 1 hit. [Graphical view]
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF64586. Pro-tRNA_synth_II_C. 1 hit.
TIGRFAMs	TIGR00408. ProS_fam_I. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_METB6

Accession

Primary (citable) accession number: A7I9R7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	September 11, 2007
Last modified:	January 25, 2012
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program