Reviewed, UniProtKB/Swiss-Prot A7I9R4 (G1PDH_METB6)

Names and origin

Protein names

Recommended name:
    Glycerol-1-phosphate dehydrogenase [NAD(P)+]
      Short name=G1P dehydrogenase
      Short name=G1PDH
    EC=1.1.1.261
Alternative name(s):
    sn-glycerol-1-phosphate dehydrogenase
    Enantiomeric glycerophosphate synthase

Gene names

Name:	egsA
Ordered Locus Names:	Mboo_1960

Organism

Methanoregula boonei (strain 6A8) [Complete proteome] [HAMAP]

Taxonomic identifier

456442 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanomicrobiales › Genera incertae sedis › Candidatus Methanoregula

Protein attributes

Sequence length	359 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NAD(P)H-dependent reduction of dihydroxyacetonephosphate (DHAP or glycerone phosphate) to glycerol-1-phosphate (G1P). The G1P thus generated is used as the glycerophosphate backbone of phospholipids in the cellular membranes of Archaea By similarity. HAMAP MF_00497
Catalytic activity	sn-glycerol-1-phosphate + NAD(P)+ = glycerone phosphate + NAD(P)H. HAMAP MF_00497
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00497
Pathway	Membrane lipid metabolism; glycerophospholipid metabolism. HAMAP MF_00497
Subcellular location	Cytoplasm Potential HAMAP MF_00497.
Sequence similarities	Belongs to the glycerol-1-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cytoplasm
Ligand	Metal-binding NAD NADP Zinc
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW phospholipid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-dehydroquinate synthase activity Inferred from electronic annotation. Source: InterPro glycerol-1-phosphate dehydrogenase [NAD(P)+] activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 359	359	Glycerol-1-phosphate dehydrogenase [NAD(P)+] HAMAP MF_00497		PRO_0000350649
Regions
	Nucleotide binding	107 – 111	5	NAD By similarity
	Nucleotide binding	129 – 132	4	NAD By similarity
Sites
	Metal binding	181	1	Zinc; catalytic By similarity
	Metal binding	261	1	Zinc; catalytic By similarity
	Metal binding	277	1	Zinc; catalytic By similarity
	Binding site	134	1	Substrate By similarity
	Binding site	138	1	NAD By similarity
	Binding site	181	1	Substrate By similarity
	Binding site	265	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7I9R4-1 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: 0DF5D14961CFA788	FASTA	359	38,384
	10 20 30 40 50 60 MSPDAIKLLK EKVFDKSKWM QLPRDVVIGH DVLGQIAPVC EDLKLGRSAL LISGKNTMDR 70 80 90 100 110 120 AGKTVQDVIG KTCDVMVYIS DEISPAVIKD AEKAAKDVDF VIGVGGGRVI DTAKIVSYNL 130 140 150 160 170 180 DRQFVSVPTA ASHDGIASAR ASVPTGEGNV SLEAHPPIAI IADTCIIASA PHRLLAAGCA 190 200 210 220 230 240 DVISNYTAIL DWEMAHRIKG EPMSEYAVAL SKMTAEILVK NADLIRPNQE QSAWFVTKAL 250 260 270 280 290 300 VSSGVAMSIA GSSRPASGGE HKFSHALDRL APNKALHGES CGIGTIISMY LHGGDWRGIR 310 320 330 340 350 QSLRTIGAPV TPTDVGIADE IAVEALLMAK TIRPERFTIF DMGITRDSAE KLIQMLYAD

References

[1]

"Complete sequence of Candidatus Methanoregula boonei 6A8." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Meincke L., Brettin T., Bruce D., Detter J.C., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Zinder S., Richardson P. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000780 Genomic DNA. Translation: ABS56475.1.
RefSeq	YP_001405118.1.
3D structure databases
SMR	A7I9R4. Positions 17-353.
ModBase	Search...
Protein-protein interaction databases
STRING	A7I9R4.
Genome annotation databases
GeneID	5409997.
GenomeReviews	Gene locus Mboo_1960 in contig CP000780_GR.
KEGG	mbn:Mboo_1960.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04488.
HOGENOM	HBG672951.
OMA	KWMQLPR.
Family and domain databases
HAMAP	MF_00497_A. G1P_dehydrogenase_A. [Tree]
InterPro	IPR002658. DHQ_synth_AroB. IPR016205. Glycerol_DH. [Graphical view]
Pfam	PF01761. DHQ_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF000112. Glycerol_dehydrogenase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

G1PDH_METB6

Accession

Primary (citable) accession number: A7I9R4

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 23, 2008
Last sequence update:	September 11, 2007
Last modified:	January 19, 2010
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)