A7I877 (A7I877_METB6) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Probable cobyrinic acid A,C-diamide synthase HAMAP-Rule MF_00027

Gene names

Name:	cobB HAMAP-Rule MF_00027
Ordered Locus Names:	Mboo_1420

Organism

Methanoregula boonei (strain 6A8) [Complete proteome] [HAMAP] EMBL ABS55938.1

Taxonomic identifier

456442 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanomicrobia › Methanomicrobiales › Methanoregulaceae › Methanoregula › Methanoregula boonei

Protein attributes

Sequence length	457 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Responsible for the amidation of carboxylic groups at position A and C of either cobyrinic acid or hydrogenobrynic acid. NH2 groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation By similarity. HAMAP-Rule MF_00027
Pathway	Cofactor biosynthesis; adenosylcobalamin biosynthesis. HAMAP-Rule MF_00027
Sequence similarities	Belongs to the CobB/CobQ family. CobB subfamily. HAMAP-Rule MF_00027 Contains 1 GATase cobBQ-type domain. HAMAP-Rule MF_00027

Ontologies

Keywords
Biological process	Cobalamin biosynthesis HAMAP-Rule MF_00027
Domain	Glutamine amidotransferase HAMAP-Rule MF_00027
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cobalamin biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	cobalamin-transporting ATPase activity Inferred from electronic annotation. Source: HAMAP cobyrinic acid a,c-diamide synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Domain	248 – 444	197	GATase cobBQ-type By similarity HAMAP-Rule MF_00027
Sites
	Active site	329	1	Nucleophile By similarity HAMAP-Rule MF_00027
	Active site	436	1	By similarity HAMAP-Rule MF_00027

Sequences

	Sequence		Length	Mass (Da)
	A7I877 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: E8FD255D8FA4341E	FASTA	457	49,735
	10 20 30 40 50 60 MKSLLITGDR SGSGKTSITL ALAALLKKRG TVQTFKVGMD YIDPSYLAAV SGRPCRNLDN 70 80 90 100 110 120 FALGDGQIRG IFAHGCKGAD IALVEGVRGL YEGAEALDDT GSTAAIAKVL DLPVVLVVSA 130 140 150 160 170 180 QSITRSAAAI VKGFQAFDPD IRIAGIILNQ VKGGSHREKA TRAIEHYCGV PVIGAIPRME 190 200 210 220 230 240 EMQLAMRHLG LVPYREGAGC GDFDERIRTI TGIIGDHVDI DRLLALAKEG TPSARDTLFE 250 260 270 280 290 300 PAPEQDIRIA VAYDEAFNFY YADLFDLLPA LGATVEQFSP VHDRLPESDG YIIGGGYPEL 310 320 330 340 350 360 FVPELERNDR MREAIHDVSR NGTPVYAECG GLMYLTDRMV LKEGWQGRKT ELSGEMCGIF 370 380 390 400 410 420 KGETVMPAKR VVSYVEGTSS KDSPVGAARF RGHEFHYSGV TLDPSTKYAY RLSRGIGIHD 430 440 450 TLDGAVRDNT LGSYTHLHPV GSSEMFRHFT DLCRSRR

References

[1]

"Complete sequence of Candidatus Methanoregula boonei 6A8." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Meincke L., Brettin T., Bruce D., Detter J.C., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Zinder S., Richardson P. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 6A8.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000780 Genomic DNA. Translation: ABS55938.1.
RefSeq	YP_001404581.1. NC_009712.1.
3D structure databases
ProteinModelPortal	A7I877.
ModBase	Search...
Protein-protein interaction databases
STRING	456442.Mboo_1420.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABS55938; ABS55938; Mboo_1420.
GeneID	5412022.
KEGG	mbn:Mboo_1420.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1797.
HOGENOM	HOG000289958.
KO	K02224.
OMA	SMRHLGL.
ProtClustDB	CLSK949491.
Enzyme and pathway databases
BioCyc	MBOO456442:GH2T-1473-MONOMER.
UniPathway	UPA00148.
Family and domain databases
HAMAP	MF_00027. CobB.
InterPro	IPR004484. CbiA_synth. IPR017929. CobB/CobQ_GATase. IPR002586. CobQ/CobB/MinD/ParA_Nub-bd_dom. IPR011698. GATase_3. [Graphical view]
Pfam	PF01656. CbiA. 1 hit. PF07685. GATase_3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00379. cobB. 1 hit.
PROSITE	PS51274. GATASE_COBBQ. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

A7I877_METB6

Accession

Primary (citable) accession number: A7I877

Entry history

Integrated into UniProtKB/TrEMBL:	September 11, 2007
Last sequence update:	September 11, 2007
Last modified:	May 1, 2013
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)