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A7I7B2

- A7I7B2_METB6

UniProt

A7I7B2 - A7I7B2_METB6

Protein

Ribulose bisphosphate carboxylase

Gene

rbcL

Organism
Methanoregula boonei (strain 6A8)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 45 (01 Oct 2014)
      Sequence version 1 (11 Sep 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the addition of molecular CO2 and H2O to ribulose 1,5-bisphosphate (RuBP), generating two molecules of 3-phosphoglycerate (3-PGA). Functions in an archaeal AMP degradation pathway, together with AMP phosphorylase and R15P isomerase.UniRule annotation

    Catalytic activityi

    2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.UniRule annotation
    3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.UniRule annotation

    Cofactori

    Binds 1 magnesium ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei152 – 1521Proton acceptorUniRule annotation
    Binding sitei154 – 1541SubstrateUniRule annotation
    Metal bindingi178 – 1781Magnesium; via carbamate groupUniRule annotation
    Metal bindingi180 – 1801MagnesiumUniRule annotation
    Metal bindingi181 – 1811MagnesiumUniRule annotation
    Active sitei270 – 2701Proton acceptorUniRule annotation
    Binding sitei271 – 2711SubstrateUniRule annotation
    Binding sitei303 – 3031SubstrateUniRule annotation
    Sitei310 – 3101Transition state stabilizerUniRule annotation

    GO - Molecular functioni

    1. magnesium ion binding Source: UniProtKB-HAMAP
    2. ribulose-bisphosphate carboxylase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. AMP catabolic process Source: UniProtKB-HAMAP
    2. carbon fixation Source: InterPro

    Keywords - Molecular functioni

    LyaseUniRule annotationImported, OxidoreductaseUniRule annotation

    Keywords - Biological processi

    Carbon dioxide fixationUniRule annotation

    Keywords - Ligandi

    MagnesiumUniRule annotation, Metal-bindingUniRule annotation

    Enzyme and pathway databases

    BioCyciMBOO456442:GH2T-1125-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribulose bisphosphate carboxylaseUniRule annotation (EC:4.1.1.39UniRule annotation)
    Short name:
    RuBisCOUniRule annotation
    Gene namesi
    Name:rbcLUniRule annotation
    Ordered Locus Names:Mboo_1105Imported
    OrganismiMethanoregula boonei (strain 6A8)Imported
    Taxonomic identifieri456442 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanoregulaceaeMethanoregula
    ProteomesiUP000002408: Chromosome

    PTM / Processingi

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei178 – 1781N6-carboxylysineUniRule annotation

    Interactioni

    Subunit structurei

    Homodimer or homodecamer. In contrast to form I RuBisCO, the form III RuBisCO is composed solely of large subunits.UniRule annotation

    Protein-protein interaction databases

    STRINGi456442.Mboo_1105.

    Structurei

    3D structure databases

    ProteinModelPortaliA7I7B2.
    SMRiA7I7B2. Positions 3-428.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni353 – 3553Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the RuBisCO large chain family.UniRule annotation
    Belongs to the RuBisCO large chain family. Type III subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1850.
    HOGENOMiHOG000230831.
    KOiK01601.
    OMAiMSIFKGV.

    Family and domain databases

    Gene3Di3.20.20.110. 1 hit.
    3.30.70.150. 1 hit.
    InterProiIPR017712. RuBisCO_III.
    IPR020878. RuBisCo_large_chain_AS.
    IPR000685. RuBisCO_lsu_C.
    IPR017443. RuBisCO_lsu_fd_N.
    IPR017444. RuBisCO_lsu_N.
    [Graphical view]
    PfamiPF00016. RuBisCO_large. 1 hit.
    PF02788. RuBisCO_large_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF51649. SSF51649. 1 hit.
    SSF54966. SSF54966. 1 hit.
    TIGRFAMsiTIGR03326. rubisco_III. 1 hit.
    PROSITEiPS00157. RUBISCO_LARGE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A7I7B2-1 [UniParc]FASTAAdd to Basket

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    MAIDWYNEFV DPNYKPANDD LVCLFYFEPA AGITDKEAAG RIASESSTGT    50
    WTTLATLPPR MKKLEAKAFE FDGHYVKVAY PLALWEEGNA VQLLSGIAGN 100
    IFGMKALKNL RLIDATLPSA YTREFKGPHF GMDGIRKMMG IRGRPLTGAV 150
    PKPKIGFSAK EHAEIGYETW MGGFDFVKDD ENLTSTSFNR FDDRVKYMTK 200
    LRDKAEKETG EKKSAFLNIS ADVETMKKRA DLLVEYGWNY AMIDVVVAGT 250
    ASVMTMRDYC SDLGLAIHAH RAFHSAFDRN KKHGMTMYFL AKLMRLIGVS 300
    QIHTGTAVGK LTGTKTESIL LADLLRKQKI QEVSHQCLAQ DWGTIKTAFP 350
    VSSGGLHPGL VPDVLDIYGT DLVLLVSGGI HGHPKGTRAG AKATMQAIEA 400
    WQEGITLDEK AKKAKELKEA LAKWGYYKPK 430
    Length:430
    Mass (Da):47,589
    Last modified:September 11, 2007 - v1
    Checksum:i834A25FF1F256C45
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000780 Genomic DNA. Translation: ABS55623.1.
    RefSeqiYP_001404266.1. NC_009712.1.

    Genome annotation databases

    EnsemblBacteriaiABS55623; ABS55623; Mboo_1105.
    GeneIDi5411265.
    KEGGimbn:Mboo_1105.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000780 Genomic DNA. Translation: ABS55623.1 .
    RefSeqi YP_001404266.1. NC_009712.1.

    3D structure databases

    ProteinModelPortali A7I7B2.
    SMRi A7I7B2. Positions 3-428.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 456442.Mboo_1105.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABS55623 ; ABS55623 ; Mboo_1105 .
    GeneIDi 5411265.
    KEGGi mbn:Mboo_1105.

    Phylogenomic databases

    eggNOGi COG1850.
    HOGENOMi HOG000230831.
    KOi K01601.
    OMAi MSIFKGV.

    Enzyme and pathway databases

    BioCyci MBOO456442:GH2T-1125-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.110. 1 hit.
    3.30.70.150. 1 hit.
    InterProi IPR017712. RuBisCO_III.
    IPR020878. RuBisCo_large_chain_AS.
    IPR000685. RuBisCO_lsu_C.
    IPR017443. RuBisCO_lsu_fd_N.
    IPR017444. RuBisCO_lsu_N.
    [Graphical view ]
    Pfami PF00016. RuBisCO_large. 1 hit.
    PF02788. RuBisCO_large_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51649. SSF51649. 1 hit.
    SSF54966. SSF54966. 1 hit.
    TIGRFAMsi TIGR03326. rubisco_III. 1 hit.
    PROSITEi PS00157. RUBISCO_LARGE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 6A8Imported.

    Entry informationi

    Entry nameiA7I7B2_METB6
    AccessioniPrimary (citable) accession number: A7I7B2
    Entry historyi
    Integrated into UniProtKB/TrEMBL: September 11, 2007
    Last sequence update: September 11, 2007
    Last modified: October 1, 2014
    This is version 45 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Miscellaneous

    Because the Archaea possessing a type III RuBisCO are all anaerobic, it is most likely that only the carboxylase activity of RuBisCO, and not the competitive oxygenase activity (by which RuBP reacts with O2 to form one molecule of 3-phosphoglycerate and one molecule of 2-phosphoglycolate), is biologically relevant in these strains.UniRule annotation

    Keywords - Technical termi

    Complete proteome, Reference proteomeImported

    External Data

    Dasty 3