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Protein

Ribulose bisphosphate carboxylase

Gene

rbcL

Organism
Methanoregula boonei (strain DSM 21154 / JCM 14090 / 6A8)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the addition of molecular CO2 and H2O to ribulose 1,5-bisphosphate (RuBP), generating two molecules of 3-phosphoglycerate (3-PGA). Functions in an archaeal AMP degradation pathway, together with AMP phosphorylase and R15P isomerase.UniRule annotation

Catalytic activityi

2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.UniRule annotation
3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei152Proton acceptorUniRule annotation1
Binding sitei154SubstrateUniRule annotation1
Metal bindingi178Magnesium; via carbamate groupUniRule annotation1
Metal bindingi180MagnesiumUniRule annotation1
Metal bindingi181MagnesiumUniRule annotation1
Active sitei270Proton acceptorUniRule annotation1
Binding sitei271SubstrateUniRule annotation1
Binding sitei303SubstrateUniRule annotation1
Sitei310Transition state stabilizerUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

LyaseUniRule annotationImported, OxidoreductaseUniRule annotation

Keywords - Biological processi

Carbon dioxide fixationUniRule annotation

Keywords - Ligandi

MagnesiumUniRule annotation, Metal-bindingUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Ribulose bisphosphate carboxylaseUniRule annotation (EC:4.1.1.39UniRule annotation)
Short name:
RuBisCOUniRule annotation
Gene namesi
Name:rbcLUniRule annotation
Ordered Locus Names:Mboo_1105Imported
OrganismiMethanoregula boonei (strain DSM 21154 / JCM 14090 / 6A8)Imported
Taxonomic identifieri456442 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanoregulaceaeMethanoregula
Proteomesi
  • UP000002408 Componenti: Chromosome

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei178N6-carboxylysineUniRule annotation1

Interactioni

Subunit structurei

Homodimer or homodecamer. In contrast to form I RuBisCO, the form III RuBisCO is composed solely of large subunits.UniRule annotation

Protein-protein interaction databases

STRINGi456442.Mboo_1105.

Structurei

3D structure databases

ProteinModelPortaliA7I7B2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 121RuBisCO_large_NInterPro annotationAdd BLAST114
Domaini133 – 424RuBisCO_largeInterPro annotationAdd BLAST292

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni353 – 355Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the RuBisCO large chain family. Type III subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04443. Archaea.
COG1850. LUCA.
HOGENOMiHOG000230831.
KOiK01601.
OMAiKDDEIFF.

Family and domain databases

CDDicd08213. RuBisCO_large_III. 1 hit.
Gene3Di3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPiMF_01133. RuBisCO_L_type3. 1 hit.
InterProiIPR033966. RuBisCO.
IPR017712. RuBisCO_III.
IPR020878. RuBisCo_large_chain_AS.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
[Graphical view]
PfamiPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMiSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
TIGRFAMsiTIGR03326. rubisco_III. 1 hit.
PROSITEiPS00157. RUBISCO_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A7I7B2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIDWYNEFV DPNYKPANDD LVCLFYFEPA AGITDKEAAG RIASESSTGT
60 70 80 90 100
WTTLATLPPR MKKLEAKAFE FDGHYVKVAY PLALWEEGNA VQLLSGIAGN
110 120 130 140 150
IFGMKALKNL RLIDATLPSA YTREFKGPHF GMDGIRKMMG IRGRPLTGAV
160 170 180 190 200
PKPKIGFSAK EHAEIGYETW MGGFDFVKDD ENLTSTSFNR FDDRVKYMTK
210 220 230 240 250
LRDKAEKETG EKKSAFLNIS ADVETMKKRA DLLVEYGWNY AMIDVVVAGT
260 270 280 290 300
ASVMTMRDYC SDLGLAIHAH RAFHSAFDRN KKHGMTMYFL AKLMRLIGVS
310 320 330 340 350
QIHTGTAVGK LTGTKTESIL LADLLRKQKI QEVSHQCLAQ DWGTIKTAFP
360 370 380 390 400
VSSGGLHPGL VPDVLDIYGT DLVLLVSGGI HGHPKGTRAG AKATMQAIEA
410 420 430
WQEGITLDEK AKKAKELKEA LAKWGYYKPK
Length:430
Mass (Da):47,589
Last modified:September 11, 2007 - v1
Checksum:i834A25FF1F256C45
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000780 Genomic DNA. Translation: ABS55623.1.
RefSeqiWP_012106650.1. NC_009712.1.

Genome annotation databases

EnsemblBacteriaiABS55623; ABS55623; Mboo_1105.
GeneIDi5411265.
KEGGimbn:Mboo_1105.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000780 Genomic DNA. Translation: ABS55623.1.
RefSeqiWP_012106650.1. NC_009712.1.

3D structure databases

ProteinModelPortaliA7I7B2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi456442.Mboo_1105.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABS55623; ABS55623; Mboo_1105.
GeneIDi5411265.
KEGGimbn:Mboo_1105.

Phylogenomic databases

eggNOGiarCOG04443. Archaea.
COG1850. LUCA.
HOGENOMiHOG000230831.
KOiK01601.
OMAiKDDEIFF.

Family and domain databases

CDDicd08213. RuBisCO_large_III. 1 hit.
Gene3Di3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPiMF_01133. RuBisCO_L_type3. 1 hit.
InterProiIPR033966. RuBisCO.
IPR017712. RuBisCO_III.
IPR020878. RuBisCo_large_chain_AS.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
[Graphical view]
PfamiPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMiSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
TIGRFAMsiTIGR03326. rubisco_III. 1 hit.
PROSITEiPS00157. RUBISCO_LARGE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiA7I7B2_METB6
AccessioniPrimary (citable) accession number: A7I7B2
Entry historyi
Integrated into UniProtKB/TrEMBL: September 11, 2007
Last sequence update: September 11, 2007
Last modified: November 30, 2016
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Miscellaneous

Because the Archaea possessing a type III RuBisCO are all anaerobic, it is most likely that only the carboxylase activity of RuBisCO, and not the competitive oxygenase activity (by which RuBP reacts with O2 to form one molecule of 3-phosphoglycerate and one molecule of 2-phosphoglycolate), is biologically relevant in these strains.UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.