A7I6W6 (KATG_METB6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase

Gene names

Name:	katG
Ordered Locus Names:	Mboo_0959

Organism

Methanoregula boonei (strain 6A8) [Complete proteome] [HAMAP]

Taxonomic identifier

456442 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanomicrobiales › Genera incertae sedis › Methanoregula

Protein attributes

Sequence length	733 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 733	733	Catalase-peroxidase HAMAP MF_01961		PRO_0000354967
Sites
	Active site	97	1	Proton acceptor By similarity
	Metal binding	260	1	Iron (heme axial ligand) By similarity
	Site	93	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	96 ↔ 219		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-245) By similarity
	Cross-link	219 ↔ 245		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-96) By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7I6W6 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: 907E15C2961F1BAC	FASTA	733	80,523
	10 20 30 40 50 60 MTDDSTCPVT GGADKQVTGR GQSYRDWWPN QPNLRVLSQH SPRSNPMGGE FNYAEEFKKL 70 80 90 100 110 120 DFAAVKADLR ALMTSSQEWW PADFGHYGPL FIRMAWHSAG TYRTLDGRGG AGSGQQRFPP 130 140 150 160 170 180 LNSWPDNVNL DKARRLLWPI KKKYGRKISW ADLMILAGNV ALESMGFETF GFAGGRVDTW 190 200 210 220 230 240 EPDEDVYWGS ENTWLGDKRY SGDRKLENPL AAVQMGLIYV NPEGPNGKPD PVAAAKDIRE 250 260 270 280 290 300 TFARMAMNDE ETVALIAGGH SFGKTHGAGP ASHVGPEPEA APIEQQGLGW KSSFGTGKGG 310 320 330 340 350 360 DAIGSGLEVT WTSTPTKWSN NFFRILFSYE WELTKSPAGA YQWQPKDGAG AGTIPDAHDK 370 380 390 400 410 420 NKRRAPTMLT TDLSLRFDPV YEKISRHFYE NPDQLADAFA RAWFKLTHRD MGPRTRYLGP 430 440 450 460 470 480 EVPKEALIWQ DPIPAVNHTL IGPREITFLK RKILASGLSI PELVLTAWAS ASTFRGSDKR 490 500 510 520 530 540 GGANGARIRL APQKDWEVNE PARLRKVLAV LEGIQQEFNK TATGGKKVSL ADLIVLAGCA 550 560 570 580 590 600 GVWKAAKNGG HKVTVPFTPG RMDATQEQTD VNSFAVLEPK ADGFRNYLKG PYAVSAEELL 610 620 630 640 650 660 VDKAQLLTLS APEMTVLIGG LRVIGATYGQ ATHGVFTTLP GALTNDFFTH LLDMGTEWKP 670 680 690 700 710 720 VAGNPDVFEG SDRKTGEPKW TGTRVDLIFG ANAQLRAIAE VYASADGEEK FAQDFVAAWA 730 KVMNLDRFDL AQK

References

[1]

"Complete sequence of Candidatus Methanoregula boonei 6A8." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Meincke L., Brettin T., Bruce D., Detter J.C., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Zinder S., Richardson P. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 6A8.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000780 Genomic DNA. Translation: ABS55477.1.
RefSeq	YP_001404120.1. NC_009712.1.
3D structure databases
ProteinModelPortal	A7I6W6.
SMR	A7I6W6. Positions 21-731.
ModBase	Search...
Protein-protein interaction databases
STRING	A7I6W6.
Protein family/group databases
PeroxiBase	6985. CMbCP01.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5412136.
GenomeReviews	Gene locus Mboo_0959 in contig CP000780_GR.
KEGG	mbn:Mboo_0959.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG05214.
HOGENOM	HBG285610.
OMA	KRHAPSM.
ProtClustDB	PRK15061.
Enzyme and pathway databases
BioCyc	CMET456442:MBOO_0959-MONOMER.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_METB6

Accession

Primary (citable) accession number: A7I6W6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	September 11, 2007
Last modified:	November 16, 2011
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program