A7I6J8 (A7I6J8_METB6) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Orotate phosphoribosyltransferase HAMAP MF_01208
Short name=OPRT HAMAP MF_01208
Short name=OPRTase HAMAP MF_01208
EC=2.4.2.10 HAMAP MF_01208

Gene names

Name:	pyrE HAMAP MF_01208
Ordered Locus Names:	Mboo_0841

Organism

Methanoregula boonei (strain 6A8) [Complete proteome] [HAMAP]

Taxonomic identifier

456442 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanomicrobiales › Genera incertae sedis › Methanoregula

Protein attributes

Sequence length	167 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) By similarity. HAMAP MF_01208
Catalytic activity	Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_01208
Cofactor	Magnesium By similarity. HAMAP MF_01208
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2. HAMAP MF_01208
Subunit structure	Homodimer By similarity. HAMAP MF_01208
Sequence similarities	Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily. HAMAP MF_01208

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis HAMAP MF_01208
Ligand	Magnesium HAMAP MF_01208
Molecular function	Glycosyltransferase HAMAP MF_01208 EMBL ABS55359.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP orotate phosphoribosyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Region	110 – 118	9	5-phosphoribose 1-diphosphate binding By similarity HAMAP MF_01208
Sites
	Binding site	85	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity HAMAP MF_01208
	Binding site	89	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity HAMAP MF_01208
	Binding site	91	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity HAMAP MF_01208
	Binding site	114	1	Orotate By similarity HAMAP MF_01208
	Binding site	142	1	Orotate By similarity HAMAP MF_01208

Sequences

	Sequence		Length	Mass (Da)
	A7I6J8 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: E0A1262925B3F97F	FASTA	167	17,782
	10 20 30 40 50 60 MNAIADLLVR YKAIEFGDFT LASGAKSRYY VDVKTAVTHP EFLAAIAQEI AKRYEFDVVA 70 80 90 100 110 120 GVAVGGVPLA VATALAAKKP FAIIRAEEKD HGKKNPIIGE VSGKNVLLVE DVTTSGGSAL 130 140 150 160 YGIAMLRTAG ARADRVVTVV DREQGAESLL KEQDAHLMAL VRVSEIL

References

[1]

"Complete sequence of Candidatus Methanoregula boonei 6A8." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Meincke L., Brettin T., Bruce D., Detter J.C., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Zinder S., Richardson P. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 6A8.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000780 Genomic DNA. Translation: ABS55359.1.
RefSeq	YP_001404002.1. NC_009712.1.
3D structure databases
ProteinModelPortal	A7I6J8.
ModBase	Search...
Protein-protein interaction databases
STRING	A7I6J8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5410030.
GenomeReviews	Gene locus Mboo_0841 in contig CP000780_GR.
KEGG	mbn:Mboo_0841.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG09150.
HOGENOM	HBG404341.
OMA	QTCLVIN.
ProtClustDB	PRK13811.
Family and domain databases
HAMAP	MF_01208. PyrE. [Tree]
InterPro	IPR004467. Or_phspho_trans_clade-1. IPR023031. Orotate_PribosylTferase. IPR000836. PRibTrfase. [Graphical view]
KO	K00762.
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR00336. PyrE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A7I6J8_METB6

Accession

Primary (citable) accession number: A7I6J8

Entry history

Integrated into UniProtKB/TrEMBL:	September 11, 2007
Last sequence update:	September 11, 2007
Last modified:	November 16, 2011
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)