Reviewed, UniProtKB/Swiss-Prot A7I6J7 (PUR2_METB6)

Names and origin

Protein names

Recommended name:
    Phosphoribosylamine--glycine ligase
    EC=6.3.4.13
Alternative name(s):
    GARS
    Glycinamide ribonucleotide synthetase
    Phosphoribosylglycinamide synthetase

Gene names

Name:	purD
Ordered Locus Names:	Mboo_0840

Organism

Methanoregula boonei (strain 6A8) [Complete proteome] [HAMAP]

Taxonomic identifier

456442 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanomicrobiales › Genera incertae sedis › Candidatus Methanoregula

Protein attributes

Sequence length	427 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP MF_00138
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP MF_00138
Sequence similarities	Belongs to the GARS family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine base biosynthetic process Inferred from electronic annotation. Source: InterPro purine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylamine-glycine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 427	427	Phosphoribosylamine--glycine ligase HAMAP MF_00138		PRO_1000018820
Regions
	Domain	109 – 313	205	ATP-grasp
	Nucleotide binding	136 – 191	56	ATP By similarity
Sites
	Metal binding	271	1	Magnesium or manganese 1 By similarity
	Metal binding	283	1	Magnesium or manganese 1 By similarity
	Metal binding	283	1	Magnesium or manganese 2 By similarity
	Metal binding	285	1	Magnesium or manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7I6J7-1 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: 4A2A6F9009D14EDA	FASTA	427	45,465
	10 20 30 40 50 60 MKILVVGGGG REHAIAAALS RTTGTEIYAA MARKNPGIAR LAKEICLTPE TNLDRIAAFA 70 80 90 100 110 120 QKTGVTHAFI GPEAPLEAGV VDRLSAAGVA CAGPTKAAAR IETDKAFCRN LMAEYKIEGN 130 140 150 160 170 180 PAYRVFFDPK EAIAFVRDHD GDLAVKPIGL TGGKGVRIMG EQVDRAGAIE YIREINGGVV 190 200 210 220 230 240 LEERLTGEEF TLQAFVDGTH IVPMPLVQDH KRAFEGDTGP NTGGMGSYTM PDHMLPFVTK 250 260 270 280 290 300 KDLTAALSIM VSVVAAMAHK GYPYKGILYG QFMNTAQGPK VIEFNARFGD PEAMNVLTLL 310 320 330 340 350 360 SSDLAEVVTG ITEGTLSASN VSFEKAATVC KYLVPEGYPS APKTNSPISI GETGSAITYY 370 380 390 400 410 420 ANVEEKNGTL QTLSSRTLAF VGKAPTLAEA EQIAENAASS VTGEVFHRRD IGTQALLDRR IAHMKEL

References

[1]

"Complete sequence of Candidatus Methanoregula boonei 6A8." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Meincke L., Brettin T., Bruce D., Detter J.C., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Zinder S., Richardson P. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000780 Genomic DNA. Translation: ABS55358.1.
RefSeq	YP_001404001.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A7I6J7.
Genome annotation databases
GeneID	5410029.
GenomeReviews	Gene locus Mboo_0840 in contig CP000780_GR.
KEGG	mbn:Mboo_0840.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	IEYNCRF.
Family and domain databases
HAMAP	MF_00138. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR013817. Pre-ATP_grasp. IPR020561. PRibGlycinamid_synth_ATP-grasp. IPR000115. PRibGlycinamide_synth. IPR020560. PRibGlycinamide_synth_C-dom. IPR020559. PRibGlycinamide_synth_CS. IPR020562. PRibGlycinamide_synth_N-dom. [Graphical view]
Gene3D	G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.90.600.10. Gars. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
Pfam	PF01071. GARS_A. 1 hit. PF02843. GARS_C. 1 hit. PF02844. GARS_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00877. purD. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00184. GARS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR2_METB6

Accession

Primary (citable) accession number: A7I6J7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 11, 2007
Last modified:	November 3, 2009
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)