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A7I6J7 (PUR2_METB6) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoribosylamine--glycine ligase

EC=6.3.4.13
Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase
Gene names
Name:purD
Ordered Locus Names:Mboo_0840
OrganismMethanoregula boonei (strain 6A8) [Complete proteome] [HAMAP]
Taxonomic identifier456442 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanoregulaceaeMethanoregula

Protein attributes

Sequence length427 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP-Rule MF_00138

Sequence similarities

Belongs to the GARS family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 427427Phosphoribosylamine--glycine ligase HAMAP-Rule MF_00138
PRO_1000018820

Regions

Domain109 – 313205ATP-grasp
Nucleotide binding136 – 19156ATP By similarity

Sites

Metal binding2711Magnesium or manganese 1 By similarity
Metal binding2831Magnesium or manganese 1 By similarity
Metal binding2831Magnesium or manganese 2 By similarity
Metal binding2851Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
A7I6J7 [UniParc].

Last modified September 11, 2007. Version 1.
Checksum: 4A2A6F9009D14EDA

FASTA42745,465
        10         20         30         40         50         60 
MKILVVGGGG REHAIAAALS RTTGTEIYAA MARKNPGIAR LAKEICLTPE TNLDRIAAFA 

        70         80         90        100        110        120 
QKTGVTHAFI GPEAPLEAGV VDRLSAAGVA CAGPTKAAAR IETDKAFCRN LMAEYKIEGN 

       130        140        150        160        170        180 
PAYRVFFDPK EAIAFVRDHD GDLAVKPIGL TGGKGVRIMG EQVDRAGAIE YIREINGGVV 

       190        200        210        220        230        240 
LEERLTGEEF TLQAFVDGTH IVPMPLVQDH KRAFEGDTGP NTGGMGSYTM PDHMLPFVTK 

       250        260        270        280        290        300 
KDLTAALSIM VSVVAAMAHK GYPYKGILYG QFMNTAQGPK VIEFNARFGD PEAMNVLTLL 

       310        320        330        340        350        360 
SSDLAEVVTG ITEGTLSASN VSFEKAATVC KYLVPEGYPS APKTNSPISI GETGSAITYY 

       370        380        390        400        410        420 
ANVEEKNGTL QTLSSRTLAF VGKAPTLAEA EQIAENAASS VTGEVFHRRD IGTQALLDRR 


IAHMKEL 

« Hide

References

[1]"Complete sequence of Candidatus Methanoregula boonei 6A8."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Meincke L., Brettin T., Bruce D., Detter J.C., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Kim E., Zinder S., Richardson P.
Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 6A8.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000780 Genomic DNA. Translation: ABS55358.1.
RefSeqYP_001404001.1. NC_009712.1.

3D structure databases

ProteinModelPortalA7I6J7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING456442.Mboo_0840.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABS55358; ABS55358; Mboo_0840.
GeneID5410029.
KEGGmbn:Mboo_0840.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0151.
HOGENOMHOG000033464.
KOK01945.
OMADPEAMNV.
ProtClustDBPRK00885.

Enzyme and pathway databases

BioCycMBOO456442:GH2T-859-MONOMER.
UniPathwayUPA00074; UER00125.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPMF_00138. GARS.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR00877. purD. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR2_METB6
AccessionPrimary (citable) accession number: A7I6J7
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: September 11, 2007
Last modified: February 19, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways