A7I5N7 (MTD_METB6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
F420-dependent methylenetetrahydromethanopterin dehydrogenase
Short name=MTD
EC=1.5.99.9

Alternative name(s):
Coenzyme F420-dependent N5,N10-methylenetetrahydromethanopterin dehydrogenase

Gene names

Name:	mtd
Ordered Locus Names:	Mboo_0530

Organism

Methanoregula boonei (strain 6A8) [Complete proteome] [HAMAP]

Taxonomic identifier

456442 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanomicrobiales › Genera incertae sedis › Methanoregula

Protein attributes

Sequence length	279 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible reduction of methenyl-H4MPT+ to methylene-H4MPT By similarity. HAMAP MF_00058
Catalytic activity	5,10-methylenetetrahydromethanopterin + coenzyme F420 = 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420. HAMAP MF_00058
Pathway	One-carbon metabolism; methanogenesis from CO(2); 5,10-methylene-5,6,7,8-tetrahydromethanopterin from 5,10-methenyl-5,6,7,8-tetrahydromethanopterin (coenzyme F420 route): step 1/1. HAMAP MF_00058
Sequence similarities	Belongs to the MTD family.

Ontologies

Keywords
Biological process	Methanogenesis One-carbon metabolism
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ferredoxin hydrogenase activity Inferred from electronic annotation. Source: InterPro methylenetetrahydromethanopterin dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 279	279	F420-dependent methylenetetrahydromethanopterin dehydrogenase HAMAP MF_00058		PRO_1000117809

Sequences

	Sequence		Length	Mass (Da)
	A7I5N7 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: 3AD95D795937EB70	FASTA	279	30,525
	10 20 30 40 50 60 MVVKVGVAKL GNIASGVMAE LLLDERADRE DMQTFMATSG TKLQPEDIDR VVSNMKAWKP 70 80 90 100 110 120 DFAIVVSPNG VLPGPTGARE QLAAAGIPVI IITDDVTTKK EWEEVKNSKF GYIIMKADSM 130 140 150 160 170 180 IGARREFLDP IEMADFNGNL VKVLAITGAF RKLQLALDKV IDQVKAGKKG TEIELPKIVM 190 200 210 220 230 240 TTDKAVDGEF TNDYALAKAR AAHEIAIAVA GQNVKGCFMT KEWEKYIPIV ASAHEMMRIA 250 260 270 AVLCDEAREI EKAGDGILRQ PHKKDGVLVR KTKLVAKFE

References

[1]

"Complete sequence of Candidatus Methanoregula boonei 6A8." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Meincke L., Brettin T., Bruce D., Detter J.C., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Zinder S., Richardson P. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 6A8.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000780 Genomic DNA. Translation: ABS55048.1.
RefSeq	YP_001403691.1. NC_009712.1.
3D structure databases
ProteinModelPortal	A7I5N7.
ModBase	Search...
Protein-protein interaction databases
STRING	A7I5N7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5411757.
GenomeReviews	Gene locus Mboo_0530 in contig CP000780_GR.
KEGG	mbn:Mboo_0530.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04866.
HOGENOM	HBG538567.
OMA	NPYAKAK.
ProtClustDB	PRK00994.
Enzyme and pathway databases
BioCyc	CMET456442:MBOO_0530-MONOMER.
Family and domain databases
HAMAP	MF_00058. MTD. [Tree]
InterPro	IPR002844. Methylene_DH. [Graphical view]
KO	K00319.
Pfam	PF01993. MTD. 1 hit. [Graphical view]
ProDom	PD017604. Methylene_DH. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF102324. MeTD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MTD_METB6

Accession

Primary (citable) accession number: A7I5N7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	September 11, 2007
Last modified:	November 16, 2011
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program