A7I5I8 (ILVC_METB6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ketol-acid reductoisomerase
EC=1.1.1.86

Alternative name(s):
Acetohydroxy-acid isomeroreductase
Alpha-keto-beta-hydroxylacil reductoisomerase

Gene names

Name:	ilvC
Ordered Locus Names:	Mboo_0481

Organism

Methanoregula boonei (strain 6A8) [Complete proteome] [HAMAP]

Taxonomic identifier

456442 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanomicrobiales › Genera incertae sedis › Methanoregula

Protein attributes

Sequence length	329 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. HAMAP MF_00435 (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH. HAMAP MF_00435
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. HAMAP MF_00435 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4. HAMAP MF_00435
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	coenzyme binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 329	329	Ketol-acid reductoisomerase HAMAP MF_00435		PRO_1000050527
Sites
	Active site	107	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	A7I5I8 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: 10D1C3BECD6DA701	FASTA	329	36,246
	10 20 30 40 50 60 MMKKYYESDA DLSVLSGKKI AVIGYGSQGR GQSLNLRDSG LDVIIGLRKG KSWDAAAKDG 70 80 90 100 110 120 MNVMTVAEAA KKADIIQILL PDELQAAVYK NEILPHLSEN KVLMFSHGFN IHFGQIQPPG 130 140 150 160 170 180 NVDVIMVAPK GPGFMVRRQY EEGKGVPALI AIHQDHTCKA HKTALAYAKG IGATRAVVLE 190 200 210 220 230 240 TTFREETETD LFGEQAVLCG GTASLIKAGF ETLVDAGYAP EMAYLEVCHE LKLIVDLIYE 250 260 270 280 290 300 GGLTNMRQYV SNTAQYGDMT RGPRVIGPEA YEAMEEILAE IQSGEFAKEW MLENMVNRPV 310 320 FNALTKADEE HLLEETGKEV RAMMPQFRK

References

[1]

"Complete sequence of Candidatus Methanoregula boonei 6A8." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Meincke L., Brettin T., Bruce D., Detter J.C., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Zinder S., Richardson P. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 6A8.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000780 Genomic DNA. Translation: ABS54999.1.
RefSeq	YP_001403642.1. NC_009712.1.
3D structure databases
ProteinModelPortal	A7I5I8.
SMR	A7I5I8. Positions 2-326.
ModBase	Search...
Protein-protein interaction databases
STRING	A7I5I8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5410166.
GenomeReviews	Gene locus Mboo_0481 in contig CP000780_GR.
KEGG	mbn:Mboo_0481.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04335.
HOGENOM	HBG286525.
OMA	MAYAKGI.
ProtClustDB	PRK05479.
Enzyme and pathway databases
BioCyc	CMET456442:MBOO_0481-MONOMER.
Family and domain databases
HAMAP	MF_00435. IlvC. [Tree]
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013328. DH_multihelical. IPR013116. IlvN. [Graphical view]
Gene3D	G3DSA:1.10.1040.10. Opine_DH. 1 hit.
KO	K00053.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit.
Pfam	PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view]
SUPFAM	SSF48179. 6DGDH_C_like. 1 hit.
TIGRFAMs	TIGR00465. IlvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILVC_METB6

Accession

Primary (citable) accession number: A7I5I8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 11, 2007
Last modified:	November 16, 2011
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program