A7I4I9 (A7I4I9_METB6) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA polymerase II large subunit HAMAP MF_00324
Short name=Pol II HAMAP MF_00324
EC=2.7.7.7 HAMAP MF_00324

Gene names

Name:	polC HAMAP MF_00324
Ordered Locus Names:	Mboo_0126

Organism

Methanoregula boonei (strain 6A8) [Complete proteome] [HAMAP]

Taxonomic identifier

456442 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanomicrobiales › Genera incertae sedis › Methanoregula

Protein attributes

Sequence length	1307 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase By similarity. HAMAP MF_00324
Catalytic activity	Degradation of single-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates. HAMAP MF_00324 Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_00324
Subunit structure	Heterodimer of a large subunit and a small subunit By similarity. HAMAP MF_00324
Sequence similarities	Belongs to the archaeal DNA polymerase II family. HAMAP MF_00324

Ontologies

Keywords
Biological process	DNA replication HAMAP MF_00324
Ligand	DNA-binding HAMAP MF_00324
Molecular function	DNA-directed DNA polymerase HAMAP MF_00324 Exonuclease HAMAP MF_00324 Hydrolase Nuclease Nucleotidyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme HAMAP MF_00324
Gene Ontology (GO)
Biological process	DNA catabolic process, exonucleolytic Inferred from electronic annotation. Source: HAMAP DNA-dependent DNA replication Inferred from electronic annotation. Source: HAMAP intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: HAMAP DNA binding Inferred from electronic annotation. Source: HAMAP DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	A7I4I9 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: CDC9C549044544B7	FASTA	1,307	144,498
	10 20 30 40 50 60 MLKISPEMEQ YGNGLMDGLN RAIALAKEAR ARGLDPSMEV EIPIASDLAD RVEVLVGIKG 70 80 90 100 110 120 VAARIRELES QMSREEAALK IGDDFVAKMF GEKNNNEILD HSIRTAMALL TEGVVSAPTE 130 140 150 160 170 180 GIAKVGLGKN DDGSQYLMIY YAGPIRSAGG TAQALSVLVG DYVRAKLGIG RYRPRPVEVE 190 200 210 220 230 240 RYIEEIRQYN TIMSLQYLPS EAEIRLIVEN CPVCIDGEAT EKEEVSGHRN LERVETNAVR 250 260 270 280 290 300 GGMALVLAEG IAGKARKLKS KVEKMNMAGW DWLDKLIAGA GKPSADDESH APGVKPLDKY 310 320 330 340 350 360 LRDLIGGRPV FSYPMRKGGF RLRYGRSRNT GFATAGFNPA TLHILGGFLA VGTQMKLERP 370 380 390 400 410 420 GKACGVVPVD TIEGPTVRLT GGEVIRVDDE KTALAVASKV ERILDVGEIL IAYGEFLENN 430 440 450 460 470 480 HPLVPAGYCE EWWLLEVPPG TKPPQDESEA LVQAKKGAYL YPAYTWFWDD ISVDQIRLLA 490 500 510 520 530 540 DAVSGTGAIE EETLVFPLDS TAKEALELLL IPHKVNGGMI RIKTFRAFIA GLGLDGNLKK 550 560 570 580 590 600 CETWKTAPAD AQPLALVMHL SGLLLRSRSG LRIGGRMGRP GKSKPRKMSP PPHGLFPLGE 610 620 630 640 650 660 SGGARRSFQE ASIHTEESDA HATEIDFEKE GGIIEIEVGR RRCPGCGEIT YLNRCQKCGT 670 680 690 700 710 720 HTTPINTCPK CGHEVPGERC PNCDVPATCS QRITLNVKAE YAQAMERLGI KKESIALVKG 730 740 750 760 770 780 VKGVISREKT VEAMEKGILR AQQDIYVFKD GTTRFDMIDL PLTHIRPDEV RVPVEKMREL 790 800 810 820 830 840 GYVQDINGYD LQNGKQVIEL HAQDILLSDS CADYMIKVTQ FIDDELTRLY GLPTFYNVKT 850 860 870 880 890 900 RDDLVGHLVI GLAPHTSAGV LARIVGFTRA NVGYAHPFFH AAKRRNCFYG ETKIEIFDGR 910 920 930 940 950 960 SWATFPIRKF VMENFDVSRP GLDRLGTYYS DPVRPYYTRT VDTNGGMHLR RITSVSIHRS 970 980 990 1000 1010 1020 PASMIRFVTA RNRELTVTPD HAMVVWDTGY LRKIKALEIK AGDAVPILEG GVVIADRIVT 1030 1040 1050 1060 1070 1080 AETVASLEDR VYCLTVAEDH TLVANGVFTG QCDGDEDCIM LLMDGLLNFS RSFLPQNRGG 1090 1100 1110 1120 1130 1140 TMDAPLVLTS RIDPAEIDKE SLNVDVGKSY PKELYEAGLV YAKAKDVEPL IDRVERRLGT 1150 1160 1170 1180 1190 1200 PRQLEGFFFT HDTSDISAGP LESTYTQLKT MAEKLEAELD LAEKIRAVDA DDVAERVLNT 1210 1220 1230 1240 1250 1260 HFIRDLMGNL SAFSKQKFRC TKCNTSYRRM PLAGKCTKFK GKGICNGNII PTVHEGSVKK 1270 1280 1290 1300 YLEVSRAMVK KYKVSEYCRQ RVEVLDLAIE STFGEEKQEQ LGLADFM

References

[1]

"Complete sequence of Candidatus Methanoregula boonei 6A8." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Meincke L., Brettin T., Bruce D., Detter J.C., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Zinder S., Richardson P. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 6A8.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000780 Genomic DNA. Translation: ABS54650.1.
RefSeq	YP_001403293.1. NC_009712.1.
3D structure databases
ProteinModelPortal	A7I4I9.
ModBase	Search...
Protein-protein interaction databases
STRING	A7I4I9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5410066.
GenomeReviews	Gene locus Mboo_0126 in contig CP000780_GR.
KEGG	mbn:Mboo_0126.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04008.
HOGENOM	HBG497074.
OMA	NCDGDED.
ProtClustDB	PRK14714.
Family and domain databases
HAMAP	MF_00324. DNApol_II_L_arch. [Tree]
InterPro	IPR003586. Hedgehog_hint_C. IPR003587. Hedgehog_hint_N. IPR006141. Intein_splice_site. IPR004475. PolC_DP2. IPR016033. PolC_DP2_N. [Graphical view]
KO	K02322.
Pfam	PF03833. PolC_DP2. 1 hit. [Graphical view]
PIRSF	PIRSF016275. PolC_DP2. 1 hit.
SMART	SM00305. HintC. 1 hit. SM00306. HintN. 1 hit. [Graphical view]
TIGRFAMs	TIGR01443. Intein_Cterm. 1 hit. TIGR01445. Intein_Nterm. 1 hit. TIGR00354. PolC. 1 hit.
PROSITE	PS50818. INTEIN_C_TER. 1 hit. PS50817. INTEIN_N_TER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

A7I4I9_METB6

Accession

Primary (citable) accession number: A7I4I9

Entry history

Integrated into UniProtKB/TrEMBL:	September 11, 2007
Last sequence update:	September 11, 2007
Last modified:	November 16, 2011
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)