A7I3Y7 (NAPA_CAMHC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Periplasmic nitrate reductase
EC=1.7.99.4

Gene names

Name:	napA
Ordered Locus Names:	CHAB381_1716

Organism

Campylobacter hominis (strain ATCC BAA-381 / LMG 19568 / NCTC 13146 / CH001A) [Complete proteome] [HAMAP]

Taxonomic identifier

360107 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter › Campylobacter hominis

Protein attributes

Sequence length	920 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630
Catalytic activity	Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630
Cofactor	Binds 1 4Fe-4S cluster By similarity. Binds 1 molybdenum ion per subunit By similarity. Binds 2 molybdopterin guanine dinucleotide (MGD) groups per subunit By similarity.
Subunit structure	Interacts with NapB By similarity.
Subcellular location	Periplasm By similarity.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP-Rule MF_01630
Sequence similarities	Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Ontologies

Keywords
Biological process	Electron transport Nitrate assimilation Transport
Cellular component	Periplasm
Domain	Signal
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding Molybdenum
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	Mo-molybdopterin cofactor biosynthetic process Inferred from electronic annotation. Source: HAMAP electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW nitrate assimilation Inferred from electronic annotation. Source: HAMAP
Cellular_component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP molybdenum ion binding Inferred from electronic annotation. Source: InterPro nitrate reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 29	29	Tat-type signal Potential
	Chain	30 – 920	891	Periplasmic nitrate reductase HAMAP-Rule MF_01630		PRO_1000069713
Sites
	Metal binding	42	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	45	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	49	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	77	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7I3Y7 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: 5168FBEB47C95866	FASTA	920	103,807
	10 20 30 40 50 60 MNRRDFIKST AAAAACASAG IALPANLNAE ENHGWRWDKA VCRFCGTGCG IMVATKNGKI 70 80 90 100 110 120 VAVKGDPEAP VNRGLNCIKG YFNAKIMYGD DRITEPLLRV NSKGEFDKHG KFAPVSWKKA 130 140 150 160 170 180 FDVMEKEFKK AYKEKGPTGI AVFGSGQYTI QEGYAAAKLV KAGFRSNNID PNARHCMASA 190 200 210 220 230 240 VVGFMQVFGI DEPSGCFDDI ELTDTIVTWG ANMAEMHPIL WSRVNDRKLS APEKVKVVNL 250 260 270 280 290 300 STFSSRTSSI ADIEIIFRPS TDVAIWNYIA REIVYNHPEA IDTEFLKNCE FATGPVDIGY 310 320 330 340 350 360 GMRNNPNHPK FSEAEKDTVS HQVSKKLSQA EGVSLAYLGL KAGDTLEMKN AKKAGKHWAI 370 380 390 400 410 420 SFEEFKKALA PYTLDYVAEI AKGDENESIE QFKEKLQNLA NLYIEKNRKI VSFWTMGFNQ 430 440 450 460 470 480 HTRGTWVNEQ SYMVHFLLGK QASPGNGAFS LTGQPSACGT AREVGTFCHR LPADMVVANP 490 500 510 520 530 540 KHREITEKIW KLPAGTINPK NGSHFVGLMR DLEDGKVKFA WVQVNNPWHN TANANHWIKA 550 560 570 580 590 600 AREMDNFIVV SDCYPGISAK VADLILPSAM IYEKWGAYGN AERRTQHWRQ QVLPVGDAMS 610 620 630 640 650 660 DTWQILEFAK RFKLKDVWGE QKIDDKLTLP NVLEEAKTMG YDENATLFDI LFANDYYKGF 670 680 690 700 710 720 KPEKIKFDNS EVNGDTRNVK GSDGEVFKGY GFFIQKALWE EYRKFGLGHG HDLADFDTYH 730 740 750 760 770 780 KVRGLKWPVV DGKETSWRFN KKYDPYAKKE ETSGDFAFYG NKNKKLDKGD LIGIKGEDKV 790 800 810 820 830 840 DINNKAKIFF RPYMDPPEIP SEEYPFWLCT GRVLEHWHSG TMTMRVPELY RAVPEALCYM 850 860 870 880 890 900 NPLDAEKLKL SQGDTIWIES RRGKVKVKID TRGRNIPPVG LVYVPWFDEN VFINKVTLDA 910 920 TCPLSSETDY KKCAVKIYKA

References

[1]

"Complete genome sequence of Campylobacter hominis ATCC BAA-381, a commensal isolated from the human gastrointestinal tract." Fouts D.E., Mongodin E.F., Puiu D., Sebastian Y., Miller W.G., Mandrell R.E., Nelson K.E. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-381 / LMG 19568 / NCTC 13146 / CH001A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000776 Genomic DNA. Translation: ABS51551.1.
RefSeq	YP_001407228.1. NC_009714.1.
3D structure databases
ProteinModelPortal	A7I3Y7.
ModBase	Search...
Protein-protein interaction databases
STRING	360107.CHAB381_1716.
Proteomic databases
PRIDE	A7I3Y7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABS51551; ABS51551; CHAB381_1716.
GeneID	5408979.
KEGG	cha:CHAB381_1716.
PATRIC	20041538. VBICamHom81367_1635.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0243.
HOGENOM	HOG000031441.
KO	K02567.
OMA	HWIKAAR.
ProtClustDB	PRK13532.
Enzyme and pathway databases
BioCyc	CHOM360107:GHCX-1721-MONOMER.
Family and domain databases
Gene3D	2.40.40.20. 2 hits.
HAMAP	MF_01630. Nitrate_reduct.
InterPro	IPR009010. Asp_de-COase-like_dom. IPR006657. MoPterin_dinucl-bd_dom. IPR006656. Mopterin_OxRdtase. IPR006963. Mopterin_OxRdtase_Fe4S4_dom. IPR006655. Mopterin_OxRdtase_prok_CS. IPR010051. Periplasm_NO3_reductase_lsu. IPR006311. TAT_signal. IPR019546. TAT_signal_bac_arc. [Graphical view]
Pfam	PF04879. Molybdop_Fe4S4. 1 hit. PF00384. Molybdopterin. 1 hit. PF01568. Molydop_binding. 1 hit. PF10518. TAT_signal. 1 hit. [Graphical view]
SMART	SM00926. Molybdop_Fe4S4. 1 hit. [Graphical view]
SUPFAM	SSF50692. Asp_decarb_fold. 1 hit.
TIGRFAMs	TIGR01706. NAPA. 1 hit. TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS00551. MOLYBDOPTERIN_PROK_1. 1 hit. PS00490. MOLYBDOPTERIN_PROK_2. False negative. PS00932. MOLYBDOPTERIN_PROK_3. False negative. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAPA_CAMHC

Accession

Primary (citable) accession number: A7I3Y7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	September 11, 2007
Last modified:	May 1, 2013
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program