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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Campylobacter hominis (strain ATCC BAA-381 / LMG 19568 / NCTC 13146 / CH001A)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi42 – 421Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi45 – 451Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi49 – 491Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi77 – 771Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. electron carrier activity Source: UniProtKB-HAMAP
  3. iron ion binding Source: UniProtKB-HAMAP
  4. molybdenum ion binding Source: InterPro
  5. nitrate reductase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
  2. nitrate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciCHOM360107:GHCX-1721-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:CHAB381_1716
OrganismiCampylobacter hominis (strain ATCC BAA-381 / LMG 19568 / NCTC 13146 / CH001A)
Taxonomic identifieri360107 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
ProteomesiUP000002407 Componenti: Chromosome

Subcellular locationi

Periplasm UniRule annotation

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929Tat-type signalUniRule annotationAdd
BLAST
Chaini30 – 920891Periplasmic nitrate reductasePRO_1000069713Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Proteomic databases

PRIDEiA7I3Y7.

Interactioni

Subunit structurei

Interacts with NapB.UniRule annotation

Protein-protein interaction databases

STRINGi360107.CHAB381_1716.

Structurei

3D structure databases

ProteinModelPortaliA7I3Y7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini35 – 91574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiKITWDKA.
OrthoDBiEOG6CVV7G.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
PF10518. TAT_signal. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A7I3Y7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRRDFIKST AAAAACASAG IALPANLNAE ENHGWRWDKA VCRFCGTGCG
60 70 80 90 100
IMVATKNGKI VAVKGDPEAP VNRGLNCIKG YFNAKIMYGD DRITEPLLRV
110 120 130 140 150
NSKGEFDKHG KFAPVSWKKA FDVMEKEFKK AYKEKGPTGI AVFGSGQYTI
160 170 180 190 200
QEGYAAAKLV KAGFRSNNID PNARHCMASA VVGFMQVFGI DEPSGCFDDI
210 220 230 240 250
ELTDTIVTWG ANMAEMHPIL WSRVNDRKLS APEKVKVVNL STFSSRTSSI
260 270 280 290 300
ADIEIIFRPS TDVAIWNYIA REIVYNHPEA IDTEFLKNCE FATGPVDIGY
310 320 330 340 350
GMRNNPNHPK FSEAEKDTVS HQVSKKLSQA EGVSLAYLGL KAGDTLEMKN
360 370 380 390 400
AKKAGKHWAI SFEEFKKALA PYTLDYVAEI AKGDENESIE QFKEKLQNLA
410 420 430 440 450
NLYIEKNRKI VSFWTMGFNQ HTRGTWVNEQ SYMVHFLLGK QASPGNGAFS
460 470 480 490 500
LTGQPSACGT AREVGTFCHR LPADMVVANP KHREITEKIW KLPAGTINPK
510 520 530 540 550
NGSHFVGLMR DLEDGKVKFA WVQVNNPWHN TANANHWIKA AREMDNFIVV
560 570 580 590 600
SDCYPGISAK VADLILPSAM IYEKWGAYGN AERRTQHWRQ QVLPVGDAMS
610 620 630 640 650
DTWQILEFAK RFKLKDVWGE QKIDDKLTLP NVLEEAKTMG YDENATLFDI
660 670 680 690 700
LFANDYYKGF KPEKIKFDNS EVNGDTRNVK GSDGEVFKGY GFFIQKALWE
710 720 730 740 750
EYRKFGLGHG HDLADFDTYH KVRGLKWPVV DGKETSWRFN KKYDPYAKKE
760 770 780 790 800
ETSGDFAFYG NKNKKLDKGD LIGIKGEDKV DINNKAKIFF RPYMDPPEIP
810 820 830 840 850
SEEYPFWLCT GRVLEHWHSG TMTMRVPELY RAVPEALCYM NPLDAEKLKL
860 870 880 890 900
SQGDTIWIES RRGKVKVKID TRGRNIPPVG LVYVPWFDEN VFINKVTLDA
910 920
TCPLSSETDY KKCAVKIYKA
Length:920
Mass (Da):103,807
Last modified:September 10, 2007 - v1
Checksum:i5168FBEB47C95866
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000776 Genomic DNA. Translation: ABS51551.1.
RefSeqiYP_001407228.1. NC_009714.1.

Genome annotation databases

EnsemblBacteriaiABS51551; ABS51551; CHAB381_1716.
KEGGicha:CHAB381_1716.
PATRICi20041538. VBICamHom81367_1635.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000776 Genomic DNA. Translation: ABS51551.1.
RefSeqiYP_001407228.1. NC_009714.1.

3D structure databases

ProteinModelPortaliA7I3Y7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi360107.CHAB381_1716.

Proteomic databases

PRIDEiA7I3Y7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABS51551; ABS51551; CHAB381_1716.
KEGGicha:CHAB381_1716.
PATRICi20041538. VBICamHom81367_1635.

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiKITWDKA.
OrthoDBiEOG6CVV7G.

Enzyme and pathway databases

BioCyciCHOM360107:GHCX-1721-MONOMER.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
PF10518. TAT_signal. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of Campylobacter hominis ATCC BAA-381, a commensal isolated from the human gastrointestinal tract."
    Fouts D.E., Mongodin E.F., Puiu D., Sebastian Y., Miller W.G., Mandrell R.E., Nelson K.E.
    Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-381 / LMG 19568 / NCTC 13146 / CH001A.

Entry informationi

Entry nameiNAPA_CAMHC
AccessioniPrimary (citable) accession number: A7I3Y7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 4, 2008
Last sequence update: September 10, 2007
Last modified: March 31, 2015
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.