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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Campylobacter hominis (strain ATCC BAA-381 / LMG 19568 / NCTC 13146 / CH001A)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase complex NapAB. Receives electrons from NapB and catalyzes the reduction of nitrate to nitrite.UniRule annotation

Catalytic activityi

2 ferrocytochrome + nitrate + 2 H+ = 2 ferricytochrome + nitrite.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi42Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi45Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi49Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi77Iron-sulfur (4Fe-4S)UniRule annotation1
Binding sitei79Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei147Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei172Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei176Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei416Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei420Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei526Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei574Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei601Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei886Substrate; via amide nitrogenUniRule annotation1
Binding sitei894Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei911Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Nitrate assimilation, Transport
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.9.6.1UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:CHAB381_1716
OrganismiCampylobacter hominis (strain ATCC BAA-381 / LMG 19568 / NCTC 13146 / CH001A)
Taxonomic identifieri360107 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
Proteomesi
  • UP000002407 Componenti: Chromosome

Subcellular locationi

  • Periplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29Tat-type signalUniRule annotationAdd BLAST29
ChainiPRO_100006971330 – 920Periplasmic nitrate reductaseUniRule annotationAdd BLAST891

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.UniRule annotation

Proteomic databases

PRIDEiA7I3Y7

Interactioni

Subunit structurei

Component of the periplasmic nitrate reductase NapAB complex composed of NapA and NapB.UniRule annotation

Protein-protein interaction databases

STRINGi360107.CHAB381_1716

Structurei

3D structure databases

ProteinModelPortaliA7I3Y7
SMRiA7I3Y7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini35 – 914Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd BLAST57

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni209 – 216Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation8
Regioni551 – 552Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation2
Regioni810 – 819Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation10

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107QIW Bacteria
COG0243 LUCA
HOGENOMiHOG000031441
KOiK02567
OMAiTQHWRQQ
OrthoDBiPOG091H060P

Family and domain databases

HAMAPiMF_01630 Nitrate_reduct_NapA, 1 hit
InterProiView protein in InterPro
IPR009010 Asp_de-COase-like_dom_sf
IPR006657 MoPterin_dinucl-bd_dom
IPR006656 Mopterin_OxRdtase
IPR006963 Mopterin_OxRdtase_4Fe-4S_dom
IPR027467 MopterinOxRdtase_cofactor_BS
IPR010051 Periplasm_NO3_reductase_lsu
IPR006311 TAT_signal
IPR019546 TAT_signal_bac_arc
PANTHERiPTHR11615:SF123 PTHR11615:SF123, 2 hits
PfamiView protein in Pfam
PF04879 Molybdop_Fe4S4, 1 hit
PF00384 Molybdopterin, 1 hit
PF01568 Molydop_binding, 1 hit
SMARTiView protein in SMART
SM00926 Molybdop_Fe4S4, 1 hit
SUPFAMiSSF50692 SSF50692, 1 hit
TIGRFAMsiTIGR01706 NAPA, 1 hit
TIGR01409 TAT_signal_seq, 1 hit
PROSITEiView protein in PROSITE
PS51669 4FE4S_MOW_BIS_MGD, 1 hit
PS00551 MOLYBDOPTERIN_PROK_1, 1 hit
PS51318 TAT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A7I3Y7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRRDFIKST AAAAACASAG IALPANLNAE ENHGWRWDKA VCRFCGTGCG
60 70 80 90 100
IMVATKNGKI VAVKGDPEAP VNRGLNCIKG YFNAKIMYGD DRITEPLLRV
110 120 130 140 150
NSKGEFDKHG KFAPVSWKKA FDVMEKEFKK AYKEKGPTGI AVFGSGQYTI
160 170 180 190 200
QEGYAAAKLV KAGFRSNNID PNARHCMASA VVGFMQVFGI DEPSGCFDDI
210 220 230 240 250
ELTDTIVTWG ANMAEMHPIL WSRVNDRKLS APEKVKVVNL STFSSRTSSI
260 270 280 290 300
ADIEIIFRPS TDVAIWNYIA REIVYNHPEA IDTEFLKNCE FATGPVDIGY
310 320 330 340 350
GMRNNPNHPK FSEAEKDTVS HQVSKKLSQA EGVSLAYLGL KAGDTLEMKN
360 370 380 390 400
AKKAGKHWAI SFEEFKKALA PYTLDYVAEI AKGDENESIE QFKEKLQNLA
410 420 430 440 450
NLYIEKNRKI VSFWTMGFNQ HTRGTWVNEQ SYMVHFLLGK QASPGNGAFS
460 470 480 490 500
LTGQPSACGT AREVGTFCHR LPADMVVANP KHREITEKIW KLPAGTINPK
510 520 530 540 550
NGSHFVGLMR DLEDGKVKFA WVQVNNPWHN TANANHWIKA AREMDNFIVV
560 570 580 590 600
SDCYPGISAK VADLILPSAM IYEKWGAYGN AERRTQHWRQ QVLPVGDAMS
610 620 630 640 650
DTWQILEFAK RFKLKDVWGE QKIDDKLTLP NVLEEAKTMG YDENATLFDI
660 670 680 690 700
LFANDYYKGF KPEKIKFDNS EVNGDTRNVK GSDGEVFKGY GFFIQKALWE
710 720 730 740 750
EYRKFGLGHG HDLADFDTYH KVRGLKWPVV DGKETSWRFN KKYDPYAKKE
760 770 780 790 800
ETSGDFAFYG NKNKKLDKGD LIGIKGEDKV DINNKAKIFF RPYMDPPEIP
810 820 830 840 850
SEEYPFWLCT GRVLEHWHSG TMTMRVPELY RAVPEALCYM NPLDAEKLKL
860 870 880 890 900
SQGDTIWIES RRGKVKVKID TRGRNIPPVG LVYVPWFDEN VFINKVTLDA
910 920
TCPLSSETDY KKCAVKIYKA
Length:920
Mass (Da):103,807
Last modified:September 11, 2007 - v1
Checksum:i5168FBEB47C95866
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000776 Genomic DNA Translation: ABS51551.1
RefSeqiWP_012109535.1, NC_009714.1

Genome annotation databases

EnsemblBacteriaiABS51551; ABS51551; CHAB381_1716
KEGGicha:CHAB381_1716

Similar proteinsi

Entry informationi

Entry nameiNAPA_CAMHC
AccessioniPrimary (citable) accession number: A7I3Y7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: September 11, 2007
Last modified: March 28, 2018
This is version 73 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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