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A7I3Y7 (NAPA_CAMHC) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Periplasmic nitrate reductase

EC=1.7.99.4
Gene names
Name:napA
Ordered Locus Names:CHAB381_1716
OrganismCampylobacter hominis (strain ATCC BAA-381 / LMG 19568 / NCTC 13146 / CH001A) [Complete proteome] [HAMAP]
Taxonomic identifier360107 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter

Protein attributes

Sequence length920 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01630

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Subunit structure

Interacts with NapB By similarity. HAMAP-Rule MF_01630

Subcellular location

Periplasm By similarity HAMAP-Rule MF_01630.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP-Rule MF_01630

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2929Tat-type signal Potential
Chain30 – 920891Periplasmic nitrate reductase HAMAP-Rule MF_01630
PRO_1000069713

Regions

Domain35 – 91574Fe-4S Mo/W bis-MGD-type

Sites

Metal binding421Iron-sulfur (4Fe-4S) By similarity
Metal binding451Iron-sulfur (4Fe-4S) By similarity
Metal binding491Iron-sulfur (4Fe-4S) By similarity
Metal binding771Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
A7I3Y7 [UniParc].

Last modified September 11, 2007. Version 1.
Checksum: 5168FBEB47C95866

FASTA920103,807
        10         20         30         40         50         60 
MNRRDFIKST AAAAACASAG IALPANLNAE ENHGWRWDKA VCRFCGTGCG IMVATKNGKI 

        70         80         90        100        110        120 
VAVKGDPEAP VNRGLNCIKG YFNAKIMYGD DRITEPLLRV NSKGEFDKHG KFAPVSWKKA 

       130        140        150        160        170        180 
FDVMEKEFKK AYKEKGPTGI AVFGSGQYTI QEGYAAAKLV KAGFRSNNID PNARHCMASA 

       190        200        210        220        230        240 
VVGFMQVFGI DEPSGCFDDI ELTDTIVTWG ANMAEMHPIL WSRVNDRKLS APEKVKVVNL 

       250        260        270        280        290        300 
STFSSRTSSI ADIEIIFRPS TDVAIWNYIA REIVYNHPEA IDTEFLKNCE FATGPVDIGY 

       310        320        330        340        350        360 
GMRNNPNHPK FSEAEKDTVS HQVSKKLSQA EGVSLAYLGL KAGDTLEMKN AKKAGKHWAI 

       370        380        390        400        410        420 
SFEEFKKALA PYTLDYVAEI AKGDENESIE QFKEKLQNLA NLYIEKNRKI VSFWTMGFNQ 

       430        440        450        460        470        480 
HTRGTWVNEQ SYMVHFLLGK QASPGNGAFS LTGQPSACGT AREVGTFCHR LPADMVVANP 

       490        500        510        520        530        540 
KHREITEKIW KLPAGTINPK NGSHFVGLMR DLEDGKVKFA WVQVNNPWHN TANANHWIKA 

       550        560        570        580        590        600 
AREMDNFIVV SDCYPGISAK VADLILPSAM IYEKWGAYGN AERRTQHWRQ QVLPVGDAMS 

       610        620        630        640        650        660 
DTWQILEFAK RFKLKDVWGE QKIDDKLTLP NVLEEAKTMG YDENATLFDI LFANDYYKGF 

       670        680        690        700        710        720 
KPEKIKFDNS EVNGDTRNVK GSDGEVFKGY GFFIQKALWE EYRKFGLGHG HDLADFDTYH 

       730        740        750        760        770        780 
KVRGLKWPVV DGKETSWRFN KKYDPYAKKE ETSGDFAFYG NKNKKLDKGD LIGIKGEDKV 

       790        800        810        820        830        840 
DINNKAKIFF RPYMDPPEIP SEEYPFWLCT GRVLEHWHSG TMTMRVPELY RAVPEALCYM 

       850        860        870        880        890        900 
NPLDAEKLKL SQGDTIWIES RRGKVKVKID TRGRNIPPVG LVYVPWFDEN VFINKVTLDA 

       910        920 
TCPLSSETDY KKCAVKIYKA 

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References

[1]"Complete genome sequence of Campylobacter hominis ATCC BAA-381, a commensal isolated from the human gastrointestinal tract."
Fouts D.E., Mongodin E.F., Puiu D., Sebastian Y., Miller W.G., Mandrell R.E., Nelson K.E.
Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-381 / LMG 19568 / NCTC 13146 / CH001A.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000776 Genomic DNA. Translation: ABS51551.1.
RefSeqYP_001407228.1. NC_009714.1.

3D structure databases

ProteinModelPortalA7I3Y7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING360107.CHAB381_1716.

Proteomic databases

PRIDEA7I3Y7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABS51551; ABS51551; CHAB381_1716.
GeneID5408979.
KEGGcha:CHAB381_1716.
PATRIC20041538. VBICamHom81367_1635.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0243.
HOGENOMHOG000031441.
KOK02567.
OMAFNQHQRG.
OrthoDBEOG6CVV7G.
ProtClustDBPRK13532.

Enzyme and pathway databases

BioCycCHOM360107:GHCX-1721-MONOMER.

Family and domain databases

HAMAPMF_01630. Nitrate_reduct.
InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
PF10518. TAT_signal. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
TIGRFAMsTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAPA_CAMHC
AccessionPrimary (citable) accession number: A7I3Y7
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: September 11, 2007
Last modified: February 19, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families