Reviewed, UniProtKB/Swiss-Prot A7I243 (NADE_CAMHC)

Names and origin

Protein names

Recommended name:
    NH(3)-dependent NAD(+) synthetase
    EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	CHAB381_1025

Organism

Campylobacter hominis (strain ATCC BAA-381 / LMG 19568 / NCTC 13146 / CH001A) [Complete proteome] [HAMAP]

Taxonomic identifier

360107 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	247 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 247	247	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_1000077539
Regions
	Nucleotide binding	29 – 36	8	ATP By similarity
Sites
	Active site	31	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7I243-1 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: D26C2A2DB6631F10	FASTA	247	27,810
	10 20 30 40 50 60 MNFIKTEKKL IKFLKKSLSK TYTENFIVGI SGGLDSAVVS ALCAKAKPKN TFGLILPAKS 70 80 90 100 110 120 SDKKNLNDAI SHCEKWGIKY EIISIEPFLQ AFRDSRNDEI SRIRLGNFAA RVRMSLLYDF 130 140 150 160 170 180 SAKISGVVVG TSNLSERMLG YGTIYGDLAC AFNPIGELFK TEIFEFAKFL NIDEKIISKA 190 200 210 220 230 240 PSADLWEGQS DEGDLGYDYA SLDEILRAIL RKKSLAKFDQ ETINFIQNRI KNNKFKLKMP KILHLKG

References

[1]

"Complete genome sequence of Campylobacter hominis ATCC BAA-381, a commensal isolated from the human gastrointestinal tract." Fouts D.E., Mongodin E.F., Puiu D., Sebastian Y., Miller W.G., Mandrell R.E., Nelson K.E. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000776 Genomic DNA. Translation: ABS52233.1.
RefSeq	YP_001406584.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A7I243.
Genome annotation databases
GeneID	5409147.
GenomeReviews	Gene locus CHAB381_1025 in contig CP000776_GR.
KEGG	cha:CHAB381_1025.
NMPDR	fig|360107.5.peg.1007.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	CAINPIG.
Family and domain databases
HAMAP	MF_00193. [Tree]
InterPro	IPR003694. NAD_synthase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. nadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_CAMHC

Accession

Primary (citable) accession number: A7I243

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	September 11, 2007
Last modified:	November 3, 2009
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)