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A7I1V2 (ISPDF_CAMHC) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 35. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Bifunctional enzyme IspD/IspF

Including the following 2 domains:

  1. 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
    EC=2.7.7.60
    Alternative name(s):
    4-diphosphocytidyl-2C-methyl-D-erythritol synthase
    MEP cytidylyltransferase
    Short name=MCT
  2. 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
    Short name=MECDP-synthase
    Short name=MECPS
    EC=4.6.1.12
Gene names
Name:ispDF
Ordered Locus Names:CHAB381_0932
OrganismCampylobacter hominis (strain ATCC BAA-381 / LMG 19568 / NCTC 13146 / CH001A) [Complete proteome] [HAMAP]
Taxonomic identifier360107 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length371 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Bifunctional enzyme that catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) (IspD), and converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP (IspF) By similarity. HAMAP MF_01520

Catalytic activity

CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_01520

2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + CMP. HAMAP MF_01520

Cofactor

Divalent metal cations By similarity. HAMAP MF_01520

Pathway

Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_01520

Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 4/6.

Sequence similarities

In the N-terminal section; belongs to the IspD family.

In the C-terminal section; belongs to the IspF family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 371371Bifunctional enzyme IspD/IspF HAMAP MF_01520
PRO_1000068625

Regions

Region1 – 2122122-C-methyl-D-erythritol 4-phosphate cytidylyltransferase HAMAP MF_01520
Region213 – 3711592-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase HAMAP MF_01520

Sites

Metal binding2191Divalent metal cation By similarity
Metal binding2211Divalent metal cation By similarity
Metal binding2531Divalent metal cation By similarity
Site161Transition state stabilizer By similarity
Site231Transition state stabilizer By similarity
Site1411Positions MEP for the nucleophilic attack By similarity
Site1931Positions MEP for the nucleophilic attack By similarity
Site2451Transition state stabilizer By similarity
Site3421Transition state stabilizer By similarity

Sequences

Sequence LengthMass (Da)Tools
A7I1V2 [UniParc].

Last modified September 11, 2007. Version 1.
Checksum: 92FAD6F69879A9C7

FASTA37140,914
        10         20         30         40         50         60 
MKDITLVLLA AGDSTRFKAP FKKQWIRIGE IPLWQYVAKD LSQKCDFSDI IIVANEKEIS 

        70         80         90        100        110        120 
YMKKIERNFK YAKGGKLRQN SLANALKKVK SEFVFVSDTA RAEISKDLIG RLINECEKFD 

       130        140        150        160        170        180 
CVSPFLGVVD TTYLGRNQID RNDLKLIQTP QISRANLLKK ALESNEIFTD DSAAVAFAGG 

       190        200        210        220        230        240 
KLGFVEGDEK ARKITFTSDL AHFDFTPASG TIFTGNGFDV HAFAKGEFIT LGGVKIPCEY 

       250        260        270        280        290        300 
SLVGHSDADA AIHALMDAIL GACGLGDIGE LFPDTDDSFK GIDSKILLQK VVNFVHLLGF 

       310        320        330        340        350        360 
KIINADITII AQKPKISPYK EKMCEILSEI LQTSRVNVKA STTEKLGFVG RVEGVAAIAS 

       370 
ANLGYFDWRK F

« Hide

References

[1]"Complete genome sequence of Campylobacter hominis ATCC BAA-381, a commensal isolated from the human gastrointestinal tract."
Fouts D.E., Mongodin E.F., Puiu D., Sebastian Y., Miller W.G., Mandrell R.E., Nelson K.E.
Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-381 / LMG 19568 / NCTC 13146 / CH001A.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000776 Genomic DNA. Translation: ABS51641.1.
RefSeqYP_001406493.1. NC_009714.1.

3D structure databases

ProteinModelPortalA7I1V2.
SMRA7I1V2. Positions 212-363.
ModBaseSearch...

Protein-protein interaction databases

STRINGA7I1V2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID5409534.
GenomeReviewsGene locus CHAB381_0932 in contig CP000776_GR.
KEGGcha:CHAB381_0932.
NMPDRfig|360107.5.peg.916.
PATRIC20039998. VBICamHom81367_0894.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1211.
HOGENOMHBG672839.
OMAGGDIGEW.
ProtClustDBPRK09382.

Enzyme and pathway databases

BioCycCHOM360107:CHAB381_0932-MONOMER.

Family and domain databases

HAMAPMF_01520. IspDF.
[Tree]
InterProIPR023423. IpsF_dom.
IPR001228. ISPD_synthase.
IPR003526. MECDP_synthase.
IPR020555. MECDP_synthase_CS.
[Graphical view]
Gene3DG3DSA:3.30.1330.50. MECDP_synthase_core. 1 hit.
KOK12506.
PfamPF01128. IspD. 1 hit.
PF02542. YgbB. 1 hit.
[Graphical view]
SUPFAMSSF69765. YgbB_synth. 1 hit.
TIGRFAMsTIGR00151. IspF. 1 hit.
PROSITEPS01295. ISPD. False negative.
PS01350. ISPF. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameISPDF_CAMHC
AccessionPrimary (citable) accession number: A7I1V2
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: September 11, 2007
Last modified: January 25, 2012
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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