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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Campylobacter hominis (strain ATCC BAA-381 / LMG 19568 / NCTC 13146 / CH001A)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei569Aminoacyl-adenylateUniRule annotation1
Binding sitei613ATPUniRule annotation1
Metal bindingi896ZincUniRule annotation1
Metal bindingi899ZincUniRule annotation1
Metal bindingi911ZincUniRule annotation1
Metal bindingi914ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:CHAB381_0906
OrganismiCampylobacter hominis (strain ATCC BAA-381 / LMG 19568 / NCTC 13146 / CH001A)
Taxonomic identifieri360107 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
Proteomesi
  • UP000002407 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000220551 – 921Isoleucine--tRNA ligaseAdd BLAST921

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi360107.CHAB381_0906

Structurei

3D structure databases

ProteinModelPortaliA7I1S7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi59 – 69"HIGH" regionAdd BLAST11
Motifi610 – 614"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246402
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

A7I1S7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYKDTLFLP TTDFAMRGNL PQNEPKRFKA WYNERKVYEK MKAKRQSAGV
60 70 80 90 100
SFNLHDGPPY ANGHLHIGHA LNKILKDIIV KTHYFAGENV RYVPGWDCHG
110 120 130 140 150
LPIEQQIEIK LGGKKNETSK TKIREMCRAH AREFINIQRD EFKDMGIIGD
160 170 180 190 200
WDDPYITMKF KFEAEIYKGL CEIAKKGLLI ERSKPVFWSW AAGSALAEAE
210 220 230 240 250
VEYKDKEDYS IYIAFDLSDE ANKKIGAKGA KAVIWTTTPW TIPANQAICL
260 270 280 290 300
NPDEIYVLTS ENFIFAKHLL ENLVQKGISK GKIVKEFASG ELENLYAKNP
310 320 330 340 350
LNDRPSKFIL GEHVLMDGGT GLVHTAPGHG EDDYYVSLKY GIETIMPVDD
360 370 380 390 400
AGKYDETIKT KKLFRDNVVD EFVGMHIFKA NEKIVELLGD AVVSVGKFTH
410 420 430 440 450
SYPYCWRTHK PVIYRATKQW FIAMDKPFKD GKTLREVALN ALKEVKFYPE
460 470 480 490 500
VGRNRITSMI ENRPDWCISR QRDWGVPIAF FRDKATKEPI FDDEILNNIY
510 520 530 540 550
EIFKEKGADA WWELDIGELL PKNCKYEAKN LEKVVDILDV WFDSGSTWRA
560 570 580 590 600
VLKSGDYDAG EFRADMYLEG SDQHRGWFQS SLLVSSAINE TAPYKSILTH
610 620 630 640 650
GFTVDEKGQK MSKSVGNVIA PQEVAKKYGV EIMRLWVGLS DYSSDLKISD
660 670 680 690 700
NILKQVSEQY RKIRNTIRFL LANVSDLKEI ETNNFTMLDK WILSKANIAF
710 720 730 740 750
EETNRAFRNY DFSKGFSVLL NFLSADLSGI YLDVCKDRLY CDELDSSRRR
760 770 780 790 800
SAQSTMVLIT RTLLPLIAPT LTYTVDEVME FAPEIVKNGK KDAFDLVYEP
810 820 830 840 850
LEFEDFKKNE TGELFIESRA KFFEIIDTLK KEKKIKSTLE LVLETSSNEI
860 870 880 890 900
LSHDLDEICD WYMVSHMRSI ESRDFLAEFK VEDETFRLVL SDHHKCPRCW
910 920
KFDAKNEDEL CPRCQKVLNV K
Length:921
Mass (Da):106,166
Last modified:September 11, 2007 - v1
Checksum:iFEB0BE766577CAF5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000776 Genomic DNA Translation: ABS51595.1
RefSeqiWP_012108759.1, NC_009714.1

Genome annotation databases

EnsemblBacteriaiABS51595; ABS51595; CHAB381_0906
KEGGicha:CHAB381_0906

Similar proteinsi

Entry informationi

Entry nameiSYI_CAMHC
AccessioniPrimary (citable) accession number: A7I1S7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: September 11, 2007
Last modified: March 28, 2018
This is version 74 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health