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A7I1S7

- SYI_CAMHC

UniProt

A7I1S7 - SYI_CAMHC

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Campylobacter hominis (strain ATCC BAA-381 / LMG 19568 / NCTC 13146 / CH001A)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 52 (01 Oct 2014)
      Sequence version 1 (11 Sep 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei569 – 5691Aminoacyl-adenylateUniRule annotation
    Binding sitei613 – 6131ATPUniRule annotation
    Metal bindingi896 – 8961ZincUniRule annotation
    Metal bindingi899 – 8991ZincUniRule annotation
    Metal bindingi911 – 9111ZincUniRule annotation
    Metal bindingi914 – 9141ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciCHOM360107:GHCX-910-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:CHAB381_0906
    OrganismiCampylobacter hominis (strain ATCC BAA-381 / LMG 19568 / NCTC 13146 / CH001A)
    Taxonomic identifieri360107 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
    ProteomesiUP000002407: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 921921Isoleucine--tRNA ligasePRO_1000022055Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi360107.CHAB381_0906.

    Structurei

    3D structure databases

    ProteinModelPortaliA7I1S7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi59 – 6911"HIGH" regionAdd
    BLAST
    Motifi610 – 6145"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A7I1S7-1 [UniParc]FASTAAdd to Basket

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    MDYKDTLFLP TTDFAMRGNL PQNEPKRFKA WYNERKVYEK MKAKRQSAGV    50
    SFNLHDGPPY ANGHLHIGHA LNKILKDIIV KTHYFAGENV RYVPGWDCHG 100
    LPIEQQIEIK LGGKKNETSK TKIREMCRAH AREFINIQRD EFKDMGIIGD 150
    WDDPYITMKF KFEAEIYKGL CEIAKKGLLI ERSKPVFWSW AAGSALAEAE 200
    VEYKDKEDYS IYIAFDLSDE ANKKIGAKGA KAVIWTTTPW TIPANQAICL 250
    NPDEIYVLTS ENFIFAKHLL ENLVQKGISK GKIVKEFASG ELENLYAKNP 300
    LNDRPSKFIL GEHVLMDGGT GLVHTAPGHG EDDYYVSLKY GIETIMPVDD 350
    AGKYDETIKT KKLFRDNVVD EFVGMHIFKA NEKIVELLGD AVVSVGKFTH 400
    SYPYCWRTHK PVIYRATKQW FIAMDKPFKD GKTLREVALN ALKEVKFYPE 450
    VGRNRITSMI ENRPDWCISR QRDWGVPIAF FRDKATKEPI FDDEILNNIY 500
    EIFKEKGADA WWELDIGELL PKNCKYEAKN LEKVVDILDV WFDSGSTWRA 550
    VLKSGDYDAG EFRADMYLEG SDQHRGWFQS SLLVSSAINE TAPYKSILTH 600
    GFTVDEKGQK MSKSVGNVIA PQEVAKKYGV EIMRLWVGLS DYSSDLKISD 650
    NILKQVSEQY RKIRNTIRFL LANVSDLKEI ETNNFTMLDK WILSKANIAF 700
    EETNRAFRNY DFSKGFSVLL NFLSADLSGI YLDVCKDRLY CDELDSSRRR 750
    SAQSTMVLIT RTLLPLIAPT LTYTVDEVME FAPEIVKNGK KDAFDLVYEP 800
    LEFEDFKKNE TGELFIESRA KFFEIIDTLK KEKKIKSTLE LVLETSSNEI 850
    LSHDLDEICD WYMVSHMRSI ESRDFLAEFK VEDETFRLVL SDHHKCPRCW 900
    KFDAKNEDEL CPRCQKVLNV K 921
    Length:921
    Mass (Da):106,166
    Last modified:September 11, 2007 - v1
    Checksum:iFEB0BE766577CAF5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000776 Genomic DNA. Translation: ABS51595.1.
    RefSeqiYP_001406468.1. NC_009714.1.

    Genome annotation databases

    EnsemblBacteriaiABS51595; ABS51595; CHAB381_0906.
    GeneIDi5409280.
    KEGGicha:CHAB381_0906.
    PATRICi20039946. VBICamHom81367_0869.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000776 Genomic DNA. Translation: ABS51595.1 .
    RefSeqi YP_001406468.1. NC_009714.1.

    3D structure databases

    ProteinModelPortali A7I1S7.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 360107.CHAB381_0906.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABS51595 ; ABS51595 ; CHAB381_0906 .
    GeneIDi 5409280.
    KEGGi cha:CHAB381_0906.
    PATRICi 20039946. VBICamHom81367_0869.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci CHOM360107:GHCX-910-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of Campylobacter hominis ATCC BAA-381, a commensal isolated from the human gastrointestinal tract."
      Fouts D.E., Mongodin E.F., Puiu D., Sebastian Y., Miller W.G., Mandrell R.E., Nelson K.E.
      Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-381 / LMG 19568 / NCTC 13146 / CH001A.

    Entry informationi

    Entry nameiSYI_CAMHC
    AccessioniPrimary (citable) accession number: A7I1S7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: September 11, 2007
    Last modified: October 1, 2014
    This is version 52 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3