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A7I0M0 (SYL_CAMHC) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:CHAB381_0473
OrganismCampylobacter hominis (strain ATCC BAA-381 / LMG 19568 / NCTC 13146 / CH001A) [Complete proteome] [HAMAP]
Taxonomic identifier360107 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter

Protein attributes

Sequence length813 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processarginyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

leucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

arginine-tRNA ligase activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 813813Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000334740

Regions

Motif39 – 4911"HIGH" region HAMAP-Rule MF_00049
Motif582 – 5865"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5851ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A7I0M0 [UniParc].

Last modified September 11, 2007. Version 1.
Checksum: 1022D7D868CF8EEB

FASTA81393,610
        10         20         30         40         50         60 
MYDSKKLELK WQEIWDKEGI FEPKKDYNLQ KKYILSMFPY PSGRIHMGHV RNYTIGDAIS 

        70         80         90        100        110        120 
RYYRMQGYNV LQPIGFDSFG MPAENAAIKH KIHPKKWTYD NIDYMTKELF RLGFSFSKNR 

       130        140        150        160        170        180 
ILATSDPIYT KFEQEFFIKM FEKGLIYRKN AVVNWCEQDQ TVLANEQVED GKCWRCGNEV 

       190        200        210        220        230        240 
VQKEMPGYYL KITSYADELL SCLKDLQNHW PSQVITMQEN WIGKSFGLEF DFKFDDESSK 

       250        260        270        280        290        300 
KLGGVKSFKV FTTRPDTIYG MSYAALAPEH AVVKAVLEKN LVSKETADKI KKILNQSPRE 

       310        320        330        340        350        360 
RQANDKDGAF LNLFAIHPLS GKKIPVWMAN FVLAEYGGGA VMAVPAHDER DFEFAHKFNL 

       370        380        390        400        410        420 
PIIQSIGAKN GENVKLPYIE SGILVDSAEF SGLENEDAKM KIIEKFEKEK IGKRVTNYKL 

       430        440        450        460        470        480 
RDWGISRQRY WGAPIPMIKC PKCGLVPEKI KNLPVTLPDD IKITGKGNPL DKHPTWKHCT 

       490        500        510        520        530        540 
CPKCGAEAER ETDTLDTFFD SSWYFARFTS DENMWQEKGI DEKSANYWMN VDQYIGGIEH 

       550        560        570        580        590        600 
AILHLLYARF FQKVLRDLGY LRDSEPFANL LTQGMVLKDG AKMSKSKGNV VDPDEIIEKY 

       610        620        630        640        650        660 
GADTARLFIL FAAPPQKELE WNDSAVEGAY KFLNRLYERS QNVQKTREIP KIDQSSLNKA 

       670        680        690        700        710        720 
EKYARLKVYE ALQKSSEVYE KTFAFNTLIA ACMEALNALN AQENRQILTE GYFIILNLLD 

       730        740        750        760        770        780 
PIVPHIACEL SENLFGRANF TPIKILPEVF EKDEIRLAVT VNGKKRAEIE VASDLSQSEI 

       790        800        810 
LKIAKEQVLK WLENKKIIKE IYIKNKLVNL VVK 

« Hide

References

[1]"Complete genome sequence of Campylobacter hominis ATCC BAA-381, a commensal isolated from the human gastrointestinal tract."
Fouts D.E., Mongodin E.F., Puiu D., Sebastian Y., Miller W.G., Mandrell R.E., Nelson K.E.
Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-381 / LMG 19568 / NCTC 13146 / CH001A.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000776 Genomic DNA. Translation: ABS52208.1.
RefSeqYP_001406061.1. NC_009714.1.

3D structure databases

ProteinModelPortalA7I0M0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING360107.CHAB381_0473.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABS52208; ABS52208; CHAB381_0473.
GeneID5409042.
KEGGcha:CHAB381_0473.
PATRIC20039108. VBICamHom81367_0457.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycCHOM360107:GHCX-478-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR001278. Arg-tRNA-ligase.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF00750. tRNA-synt_1d. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_CAMHC
AccessionPrimary (citable) accession number: A7I0M0
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: September 11, 2007
Last modified: May 14, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries