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A7HY03 (SYE2_PARL1) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate--tRNA ligase 2

EC=6.1.1.17
Alternative name(s):
Glutamyl-tRNA synthetase 2
Short name=GluRS 2
Gene names
Name:gltX2
Ordered Locus Names:Plav_3180
OrganismParvibaculum lavamentivorans (strain DS-1 / DSM 13023 / NCIMB 13966) [Complete proteome] [HAMAP]
Taxonomic identifier402881 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhodobiaceaeParvibaculum

Protein attributes

Sequence length490 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP-Rule MF_00022

Catalytic activity

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP-Rule MF_00022

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00022

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00022.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglutamyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glutamate-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 490490Glutamate--tRNA ligase 2 HAMAP-Rule MF_00022
PRO_0000367733

Regions

Motif33 – 4311"HIGH" region HAMAP-Rule MF_00022
Motif262 – 2665"KMSKS" region HAMAP-Rule MF_00022

Sites

Binding site2651ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A7HY03 [UniParc].

Last modified September 11, 2007. Version 1.
Checksum: 9E160077E47F8F28

FASTA49055,041
        10         20         30         40         50         60 
MPTFSLVAYP RGCGAGSQEI MTEKKTVITR FAPSPTGYLH IGGARTALFN WLFARHHGGQ 

        70         80         90        100        110        120 
FLLRIEDTDR ERSTTPAIDA IFEGLQWLGL SWDAEPVFQA SRAARHVEAV QELLDRGKAY 

       130        140        150        160        170        180 
HCYASQQELE EMREKARAEG RPIHYDGRWR DRDPAEAPAG VRPVVRFRSP NDGETIVRDH 

       190        200        210        220        230        240 
VMGDVRFPNE QLDDLILLRS DGTPTYNLSV VVDDHDMSIT HIIRGDDHLT NAARQSQIYE 

       250        260        270        280        290        300 
ALGWEVPEFA HVPLIHGPDG AKLSKRHGAL GAEAYRDLGY LPEAMRNYLV RLGWSHGDDE 

       310        320        330        340        350        360 
LFSTEQAIEW FNLESIGRSP ARFDFAKLEN LNGHYIREAD DERLADETIA LMTRNEGWKT 

       370        380        390        400        410        420 
DDEFRKKLIA LMPGLKERAK TLVEIGKGAA FLLVHRPLTC DDKALQLLNP EARALLARLV 

       430        440        450        460        470        480 
SRLEARNDWI LSEIETTVRA FAEEENLKLG KVAQPLRAAV TGSTVSPPIF DVLATLGRDE 

       490 
ALGRIKDQAA 

« Hide

References

[1]"Complete genome sequence and annotation of Parvibaculum lavamentivorans DS-1."
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Richardson P.
Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DS-1 / DSM 13023 / NCIMB 13966.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000774 Genomic DNA. Translation: ABS64786.1.
RefSeqYP_001414443.1. NC_009719.1.

3D structure databases

ProteinModelPortalA7HY03.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING402881.Plav_3180.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABS64786; ABS64786; Plav_3180.
GeneID5454198.
KEGGpla:Plav_3180.
PATRIC22868157. VBIParLav90819_3285.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0008.
HOGENOMHOG000252722.
KOK01885.
OMAVTGQTHG.
OrthoDBEOG6DRPF7.

Enzyme and pathway databases

BioCycPLAV402881:GHQA-3224-MONOMER.

Family and domain databases

Gene3D1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPMF_00022_B. Glu_tRNA_synth_B.
InterProIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10119. PTHR10119. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF48163. SSF48163. 1 hit.
TIGRFAMsTIGR00464. gltX_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYE2_PARL1
AccessionPrimary (citable) accession number: A7HY03
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: September 11, 2007
Last modified: May 14, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries