Reviewed, UniProtKB/Swiss-Prot A7HXV6 (ISPDF_PARL1)

Names and origin

Protein names

Recommended name:
    Bifunctional enzyme ispD/ispF
Including the following 2 domains:
    1- Recommended name:
            2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
              EC=2.7.7.60
        Alternative name(s):
            4-diphosphocytidyl-2C-methyl-D-erythritol synthase
            MEP cytidylyltransferase
              Short name=MCT
    2- Recommended name:
            2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
                Short name=MECPS
                Short name=MECDP-synthase
              EC=4.6.1.12

Gene names

Name:	ispDF
Ordered Locus Names:	Plav_3132

Organism

Parvibaculum lavamentivorans (strain DS-1 / DSM 13023 / NCIMB 13966) [Complete proteome] [HAMAP]

Taxonomic identifier

402881 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Phyllobacteriaceae › Parvibaculum

Protein attributes

Sequence length	382 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme that catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) (ispD), and converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP (ispF) By similarity.
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_01520 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + CMP. HAMAP MF_01520
Cofactor	Divalent metal cations By similarity.
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_01520 Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 4/6.
Sequence similarities	In the N-terminal section; belongs to the ispD family. In the C-terminal section; belongs to the ispF family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	Metal-binding
Molecular function	Lyase Nucleotidyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity Inferred from electronic annotation. Source: HAMAP 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 382	382	Bifunctional enzyme ispD/ispF HAMAP MF_01520		PRO_0000315555
Regions
	Region	1 – 226	226	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase HAMAP MF_01520
	Region	227 – 382	156	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase HAMAP MF_01520
Sites
	Metal binding	233	1	Divalent metal cation By similarity
	Metal binding	235	1	Divalent metal cation By similarity
	Metal binding	267	1	Divalent metal cation By similarity
	Site	15	1	Transition state stabilizer By similarity
	Site	22	1	Transition state stabilizer By similarity
	Site	152	1	Positions MEP for the nucleophilic attack By similarity
	Site	206	1	Positions MEP for the nucleophilic attack By similarity
	Site	259	1	Transition state stabilizer By similarity
	Site	358	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7HXV6-1 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: CFAA20EE65BF6F9D	FASTA	382	39,631
	10 20 30 40 50 60 MKVAALIVAA GRGSRAGPGA PKQYRLLAGQ PVLRRTLAAF ANHPEIMTVL TVIHPEDASA 70 80 90 100 110 120 FEAASGGLPK LLEPVFGGST RQDSVRAGLE ALNADAPDLV LIHDGARPLI SAPVISACIA 130 140 150 160 170 180 ALSTHEGAQA GLSLTDTIRR TDKGMAAETV ARDTLWRAQT PQAFRFNAIL DAHRQASGGE 190 200 210 220 230 240 HTDDVSVALA AGVKVAMVEG DQDNIKITSA ADIQQAERIL MRSGETRTGL GYDVHRFGPG 250 260 270 280 290 300 DYVWLCGVKV PHDAGLVGHS DADAGLHAIT DAVLGAIGAG DIGRHFPPSD PKWKGAPSEI 310 320 330 340 350 360 FLAHAAKLAT EAGARVSNVD VTLICELPKI GPHADAMRAR IADILGIEKG RVSVKATTTE 370 380 GLGFTGRGEG LAAQAIVTLI IG

References

[1]

"Complete genome sequence and annotation of Parvibaculum lavamentivorans DS-1." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Richardson P. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000774 Genomic DNA. Translation: ABS64739.1.
RefSeq	YP_001414396.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A7HXV6.
Genome annotation databases
GeneID	5453865.
GenomeReviews	Gene locus Plav_3132 in contig CP000774_GR.
KEGG	pla:Plav_3132.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	IVLIHDA.
Family and domain databases
HAMAP	MF_01520. [Tree]
InterPro	IPR001228. ISPD_synthase. IPR018294. ISPD_synthase_CS. IPR003526. MECDP_synthase_core. IPR020555. MECDP_synthase_CS. [Graphical view]
Gene3D	G3DSA:3.30.1330.50. MECDP_synthase_core. 1 hit.
Pfam	PF01128. IspD. 1 hit. PF02542. YgbB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00453. ispD. 1 hit. TIGR00151. ispF. 1 hit.
PROSITE	PS01295. ISPD. 1 hit. PS01350. ISPF. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ISPDF_PARL1

Accession

Primary (citable) accession number: A7HXV6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 11, 2007
Last modified:	November 3, 2009
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)