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Protein

Fumarate hydratase class II

Gene

fumC

Organism
Parvibaculum lavamentivorans (strain DS-1 / DSM 13023 / NCIMB 13966)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the TCA cycle. Catalyzes the stereospecific interconversion of fumarate to L-malate.UniRule annotation

Miscellaneous

There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors.UniRule annotation

Catalytic activityi

(S)-malate = fumarate + H2O.UniRule annotation

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from fumarate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Fumarate hydratase class II (fumC)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from fumarate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei190SubstrateUniRule annotation1
Active sitei191Proton donor/acceptorUniRule annotation1
Active sitei321UniRule annotation1
Binding sitei322SubstrateUniRule annotation1
Sitei334Important for catalytic activityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyaseUniRule annotationSAAS annotation
Biological processTricarboxylic acid cycleUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00223; UER01007.

Names & Taxonomyi

Protein namesi
Recommended name:
Fumarate hydratase class IIUniRule annotation (EC:4.2.1.2UniRule annotation)
Short name:
Fumarase CUniRule annotation
Alternative name(s):
Aerobic fumaraseUniRule annotation
Iron-independent fumaraseUniRule annotation
Gene namesi
Name:fumCUniRule annotation
Ordered Locus Names:Plav_2445Imported
OrganismiParvibaculum lavamentivorans (strain DS-1 / DSM 13023 / NCIMB 13966)Imported
Taxonomic identifieri402881 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhodobiaceaeParvibaculum
Proteomesi
  • UP000006377 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi402881.Plav_2445.

Structurei

3D structure databases

ProteinModelPortaliA7HVX1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini15 – 345Lyase_1InterPro annotationAdd BLAST331
Domaini411 – 463FumaraseC_CInterPro annotationAdd BLAST53

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni101 – 103Substrate bindingUniRule annotation3
Regioni132 – 135Substrate binding (B site)UniRule annotation4
Regioni142 – 144Substrate bindingUniRule annotation3
Regioni327 – 329Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C9Q. Bacteria.
COG0114. LUCA.
HOGENOMiHOG000061736.
KOiK01679.
OMAiVSFTDNC.
OrthoDBiPOG091H01XG.

Family and domain databases

CDDicd01362. Fumarase_classII. 1 hit.
Gene3Di1.10.275.10. 1 hit.
HAMAPiMF_00743. FumaraseC. 1 hit.
InterProiView protein in InterPro
IPR005677. Fum_hydII.
IPR024083. Fumarase/histidase_N.
IPR018951. Fumarase_C_C.
IPR020557. Fumarate_lyase_CS.
IPR000362. Fumarate_lyase_fam.
IPR022761. Fumarate_lyase_N.
IPR008948. L-Aspartase-like.
PANTHERiPTHR11444. PTHR11444. 1 hit.
PfamiView protein in Pfam
PF10415. FumaraseC_C. 1 hit.
PF00206. Lyase_1. 1 hit.
PRINTSiPR00149. FUMRATELYASE.
SUPFAMiSSF48557. SSF48557. 1 hit.
TIGRFAMsiTIGR00979. fumC_II. 1 hit.
PROSITEiView protein in PROSITE
PS00163. FUMARATE_LYASES. 1 hit.

Sequencei

Sequence statusi: Complete.

A7HVX1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANTPKTRTE TDTFGPIEVA ADKYWGAQAE RSLGNFRIGW EKQPLPVVRA
60 70 80 90 100
LGIVKRAAAE ANMALGRLDP KIGEAVVKAA QEVIEGKLDD HFPLVVWQTG
110 120 130 140 150
SGTQSNMNAN EVISNRAIEI MGGEMGSKKP VHPNDHVNMS QSSNDTYPTA
160 170 180 190 200
MHVACAEEIH HRLLPALQKL RNALNNKAQA WNHIIKIGRT HTQDATPLTL
210 220 230 240 250
GQEFSGYTAQ VENGITRIEQ TLPKLMELAQ GGTAVGTGLN APVGFAEKVA
260 270 280 290 300
DNIAAITHLP FKTAPNKFEA LASHDAMVFS HGAINTVAAS LFKIANDIRL
310 320 330 340 350
LGSGPRSGLG ELSLPENEPG SSIMPGKVNP TQCEAMTQVC VQIFGNHAAL
360 370 380 390 400
TFAGSQGHFE LNVYNPVMAY NFLQSVRLMS DAAVSFTDNC VVGIEPREDN
410 420 430 440 450
IKAALERSLM LVTALAPKIG YDNAAKIAKT AHKNGTTLRE EAVGGGYVTD
460
EEFDAYVRPE KMIAPE
Length:466
Mass (Da):50,153
Last modified:September 11, 2007 - v1
Checksum:i597516943164EEEF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000774 Genomic DNA. Translation: ABS64054.1.
RefSeqiWP_012111365.1. NC_009719.1.

Genome annotation databases

EnsemblBacteriaiABS64054; ABS64054; Plav_2445.
KEGGipla:Plav_2445.

Similar proteinsi

Entry informationi

Entry nameiA7HVX1_PARL1
AccessioniPrimary (citable) accession number: A7HVX1
Entry historyiIntegrated into UniProtKB/TrEMBL: September 11, 2007
Last sequence update: September 11, 2007
Last modified: September 27, 2017
This is version 72 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported