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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Parvibaculum lavamentivorans (strain DS-1 / DSM 13023 / NCIMB 13966)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei633 – 6331ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPLAV402881:GHQA-1294-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Plav_1280
OrganismiParvibaculum lavamentivorans (strain DS-1 / DSM 13023 / NCIMB 13966)
Taxonomic identifieri402881 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhodobiaceaeParvibaculum
ProteomesiUP000006377: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 870870Leucine--tRNA ligasePRO_0000334788Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi402881.Plav_1280.

Structurei

3D structure databases

ProteinModelPortaliA7HSL7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi43 – 5311"HIGH" regionAdd
BLAST
Motifi630 – 6345"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiREKNDWE.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A7HSL7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSTRYNARAA EPKWQKIWEE RGDFLMRDDA DGKPKYYVLE MFPYPSGRIH
60 70 80 90 100
MGHVRNYTMG DVIARYKKAR GFNVLHPMGW DAFGMPAENA AMEKNVHPKG
110 120 130 140 150
WTYDNIAAMR EQLKAIGLAI DWSREFATCD PEYYGHEQAL FLDMLEAGLV
160 170 180 190 200
SRKKSMVNWD PVDNTVLANE QVIDGRGWRS GALVERRELT QWFLKISDFA
210 220 230 240 250
DELLEGLDTL DRWPEKVRLM QKNWIGRSEG ARVFFELENA PDGNTKLEIF
260 270 280 290 300
TTRPDTLYGA SFCALSPHHP LTQSLAKDNP ALTDFIRECD RIGTSEEAIE
310 320 330 340 350
TADKMGFDTG LKARHPFIEG KTLPVYVANF VLMDYGTGAI FACPAHDQRD
360 370 380 390 400
LDFARKYNLP VIPVVAPKDK QGAELDAFAS GLAETGTDAY TGDGVAINSD
410 420 430 440 450
FLNGLDVQSA KRAAIDRLEA KGIGEGTVNY RLRDWGISRQ RYWGCPIPVI
460 470 480 490 500
HCASCGTLPV PRDQLPVVLP DDVNFSEPGN PLDRHPSWKH VDCPKCGKPA
510 520 530 540 550
SRETDTFDTF VDSSWYFVRF TAPDAPTPTD KALADHWLPV DQYIGGIEHA
560 570 580 590 600
ILHLLYSRFF TRAMKATGHV SLDEPFAGLF TQGMVNHETY RDAEGRWVPP
610 620 630 640 650
AEVDIETVSG KRVAKRIADG EPVIIGSVEK MSKSKKNTVD PEDIIAKYGA
660 670 680 690 700
DTARWFMLSD SPPERDVQWT DQGAEGAWRF TQRLWRMVTE RQEDLAPTGT
710 720 730 740 750
PMPTAFSEDE LTLRRAAHQA LAAATEDFEN LRFNRAVARV YELANAVSGF
760 770 780 790 800
TPGTPEGAFA KREALEILVQ IVGPMMPHIA EECWEALGHG EPLTAAEWPV
810 820 830 840 850
ADKALLVEDS VTIAVQVNGK RRDELTIARD ADRETVERAA LALEKVGKAI
860 870
DGKPVRKVIV VPGKIVNIVV
Length:870
Mass (Da):97,257
Last modified:September 11, 2007 - v1
Checksum:i45E0A439AAE78404
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000774 Genomic DNA. Translation: ABS62900.1.
RefSeqiWP_012110174.1. NC_009719.1.
YP_001412557.1. NC_009719.1.

Genome annotation databases

EnsemblBacteriaiABS62900; ABS62900; Plav_1280.
GeneIDi5456455.
KEGGipla:Plav_1280.
PATRICi22864158. VBIParLav90819_1318.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000774 Genomic DNA. Translation: ABS62900.1.
RefSeqiWP_012110174.1. NC_009719.1.
YP_001412557.1. NC_009719.1.

3D structure databases

ProteinModelPortaliA7HSL7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi402881.Plav_1280.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABS62900; ABS62900; Plav_1280.
GeneIDi5456455.
KEGGipla:Plav_1280.
PATRICi22864158. VBIParLav90819_1318.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiREKNDWE.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciPLAV402881:GHQA-1294-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence and annotation of Parvibaculum lavamentivorans DS-1."
    Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Richardson P.
    Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DS-1 / DSM 13023 / NCIMB 13966.

Entry informationi

Entry nameiSYL_PARL1
AccessioniPrimary (citable) accession number: A7HSL7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: September 11, 2007
Last modified: January 7, 2015
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.