Reviewed, UniProtKB/Swiss-Prot A7HS42 (SYE1_PARL1)

Names and origin

Protein names

Recommended name:
    Glutamyl-tRNA synthetase 1
    EC=6.1.1.17
Alternative name(s):
    Glutamate--tRNA ligase 1
      Short name=GluRS 1

Gene names

Name:	gltX1
Ordered Locus Names:	Plav_1104

Organism

Parvibaculum lavamentivorans (strain DS-1 / DSM 13023 / NCIMB 13966) [Complete proteome] [HAMAP]

Taxonomic identifier

402881 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Phyllobacteriaceae › Parvibaculum

Protein attributes

Sequence length	449 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity.
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 449	449	Glutamyl-tRNA synthetase 1 HAMAP MF_00022		PRO_0000367730
Regions
	Motif	11 – 21	11	"HIGH" region HAMAP MF_00022
	Motif	242 – 246	5	"KMSKS" region HAMAP MF_00022
Sites
	Binding site	245	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7HS42-1 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: 214F6D8EBB9C309F	FASTA	449	49,180
	10 20 30 40 50 60 MSGAPVVRFA PSPTGSLHVG NARAALFNFL FARKNSGKFM LRMDDTDDER STVEFAAGIE 70 80 90 100 110 120 EDLTWLGLRH DLFARQSDRL AAYEAAAAKL KADGRLYPAY ETAAELDRKR KRQMARGLPP 130 140 150 160 170 180 VYDRAALALT DEDRAKLEAE GRKPHWRFRL DRVHTAFDDL VQGAVEVDGA SLSDPVLIRE 190 200 210 220 230 240 DGRFLYTLPS VVDDIDFAVT HVIRGSDHIT NTGVQIQIIR ALGAEPPVYA HYSLLNGPEG 250 260 270 280 290 300 KPLSKRDDAA RFSLRALRDA GFEPMALNSL LARLGTPDAV EPCLSLDELA ARFDISRLGR 310 320 330 340 350 360 ADIRFDPADL AKVNTACLHL MSYEEAKPRL AALGCDLGEA FWEAIKPNLI LFSDAADWAR 370 380 390 400 410 420 VVEGPVEPVI ENPDFAAAAA AALPPEPWDE STWALWTDAV KQATGAKGKA LFMPLRLALT 430 440 GLTHGPELKN LLPLIGRERA SARLGGLTR

References

[1]

"Complete genome sequence and annotation of Parvibaculum lavamentivorans DS-1." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Richardson P. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000774 Genomic DNA. Translation: ABS62725.1.
RefSeq	YP_001412382.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A7HS42.
Genome annotation databases
GeneID	5455819.
GenomeReviews	Gene locus Plav_1104 in contig CP000774_GR.
KEGG	pla:Plav_1104.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	LRLDDTD.
Family and domain databases
HAMAP	MF_00022. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ic_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ic. IPR020061. Glu/Gln-tRNA-synth_Ic_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ic_cat-dom. IPR020060. Glu/Gln-tRNA-synth_Ic_N. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
TIGRFAMs	TIGR00464. gltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE1_PARL1

Accession

Primary (citable) accession number: A7HS42

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	September 11, 2007
Last modified:	November 3, 2009
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)