A7HR11 (ISPE_PARL1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	Plav_0721

Organism

Parvibaculum lavamentivorans (strain DS-1 / DSM 13023 / NCIMB 13966) [Complete proteome] [HAMAP]

Taxonomic identifier

402881 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Phyllobacteriaceae › Parvibaculum

Protein attributes

Sequence length	290 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 290	290	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_0000335735
Regions
	Nucleotide binding	97 – 107	11	ATP Potential
Sites
	Active site	12	1	By similarity
	Active site	139	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7HR11 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: 203CB3863F592904	FASTA	290	29,806
	10 20 30 40 50 60 MSGPVTEAAR AKINLTLRVL GKRADGYHEL QSLVVFAQSG DRLTAREADE LRLDVAGRFA 70 80 90 100 110 120 AALQGEPDNL VLRAARMLRE ETGIKSGAHL TLEKNLPVAS GIGGGSADAA AALRALTALW 130 140 150 160 170 180 GVAPGDEVLS RIAAALGADV LVCLHSRTAM MWGKGEKIMP LADLPRFWLV LANAGIALST 190 200 210 220 230 240 AAVFRELAAA PLAAAPSDPA PVPPGTLDDL AAWLAAEGND LEPPALTLAP EIGETISALA 250 260 270 280 290 LTAGCLLARM SGSGATCFGL YAAEEEAREA ALVLQAAHPG WWLEVSAAGG

References

[1]

"Complete genome sequence and annotation of Parvibaculum lavamentivorans DS-1." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Richardson P. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DS-1 / DSM 13023 / NCIMB 13966.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000774 Genomic DNA. Translation: ABS62344.1.
RefSeq	YP_001412001.1. NC_009719.1.
3D structure databases
ProteinModelPortal	A7HR11.
ModBase	Search...
Protein-protein interaction databases
STRING	A7HR11.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5454644.
GenomeReviews	Gene locus Plav_0721 in contig CP000774_GR.
KEGG	pla:Plav_0721.
PATRIC	22863008. VBIParLav90819_0747.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1947.
HOGENOM	HBG734593.
OMA	KINLHLG.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_PARL1

Accession

Primary (citable) accession number: A7HR11

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	September 11, 2007
Last modified:	December 14, 2011
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program