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A7HP26 (BIOB_PARL1) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:Plav_0036
OrganismParvibaculum lavamentivorans (strain DS-1 / DSM 13023 / NCIMB 13966) [Complete proteome] [HAMAP]
Taxonomic identifier402881 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhodobiaceaeParvibaculum

Protein attributes

Sequence length338 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence caution

The sequence ABS61659.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 338338Biotin synthase HAMAP-Rule MF_01694
PRO_0000381517

Sites

Metal binding601Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding641Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding671Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1041Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1351Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1951Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2671Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
A7HP26 [UniParc].

Last modified July 28, 2009. Version 2.
Checksum: 02CC62D983635D5B

FASTA33837,421
        10         20         30         40         50         60 
MTARTDPGAL RHDWTREELQ ALFDLPFNDL LFEAQLVHRR WFKADEVQMS TLLSIKTGGC 

        70         80         90        100        110        120 
PEDCGYCAQS SKFDTGLKAS KLMEVEKVLA EARKAKEAGA TRYCMGAAWR EPKDRDMDMV 

       130        140        150        160        170        180 
CAMVEGIKDM GMETCMTLGM LSGQQVHRLA QSGLDYYNHN IDTSEEYYPK VISTRTYQDR 

       190        200        210        220        230        240 
LETLERVRNA GINVCSGGIV GMGESPSDRI GMLMTLANLE EHPQSVPINM LMPVEGTALG 

       250        260        270        280        290        300 
ASEKIDPIDF VRLIAVARIA MPQSVVRLSA GREHMSEETQ ALCFLAGANS IFIGEKLLTT 

       310        320        330 
KNPEADKDQR LFAKLGIRSM PAHSCPSEKD VSADRKAG 

« Hide

References

[1]"Complete genome sequence and annotation of Parvibaculum lavamentivorans DS-1."
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Richardson P.
Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DS-1 / DSM 13023 / NCIMB 13966.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000774 Genomic DNA. Translation: ABS61659.1. Different initiation.
RefSeqYP_001411316.1. NC_009719.1.

3D structure databases

ProteinModelPortalA7HP26.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING402881.Plav_0036.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABS61659; ABS61659; Plav_0036.
GeneID5454827.
KEGGpla:Plav_0036.
PATRIC22861572. VBIParLav90819_0038.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239957.
KOK01012.
OrthoDBEOG622PMP.

Enzyme and pathway databases

BioCycPLAV402881:GHQA-36-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_PARL1
AccessionPrimary (citable) accession number: A7HP26
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 28, 2009
Last modified: February 19, 2014
This is version 45 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways