A7HNL5 (ISPE_FERNB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	Fnod_1663

Organism

Fervidobacterium nodosum (strain ATCC 35602 / DSM 5306 / Rt17-B1) [Complete proteome] [HAMAP]

Taxonomic identifier

381764 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Thermotogae › Thermotogae › Thermotogales › Thermotogaceae › Fervidobacterium › Fervidobacterium nodosum

Protein attributes

Sequence length	272 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP-Rule MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP-Rule MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP-Rule MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway Inferred from electronic annotation. Source: UniProtKB-UniPathway terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: HAMAP ATP binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 272	272	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP-Rule MF_00061		PRO_0000335714
Regions
	Nucleotide binding	92 – 102	11	ATP Potential
Sites
	Active site	14	1	By similarity
	Active site	132	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7HNL5 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: 82042DF03EB207E7	FASTA	272	30,259
	10 20 30 40 50 60 MGESYRIVLR TYAKLNLCLD VLGKRQDGYH EINSLFQNIS LFDEMDITLS DGKGKLLINS 70 80 90 100 110 120 NVNIGNNILN SVWELVNCDN KNVYVNLQKN IPMGGGLGGG SSNAAGFIIA LEKVGIISKE 130 140 150 160 170 180 QSLKIAQKVG SDVPFFLFGG TAIVKGRGEV ILPVEPLTNF GKFKVDLHLP NFSISTKEAY 190 200 210 220 230 240 SKLKAEWFGK APITPEELYD FYKTRNFEMI KKGTYNIFER VIPMDLLEKI ESLRRDFPAA 250 260 270 LTGSGSTYFA LKEDGKYSFV PKGVEINAFE KS

References

[1]

"Complete sequence of Fervidobacterium nodosum Rt17-B1." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Mikhailova N., Nelson K., Gogarten J.P., Noll K., Richardson P. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35602 / DSM 5306 / Rt17-B1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000771 Genomic DNA. Translation: ABS61498.1.
RefSeq	YP_001411155.1. NC_009718.1.
3D structure databases
ProteinModelPortal	A7HNL5.
ModBase	Search...
Protein-protein interaction databases
STRING	381764.Fnod_1663.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABS61498; ABS61498; Fnod_1663.
GeneID	5452941.
KEGG	fno:Fnod_1663.
PATRIC	21884901. VBIFerNod12464_1728.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1947.
HOGENOM	HOG000019600.
KO	K00919.
OMA	GYHEINS.
Enzyme and pathway databases
UniPathway	UPA00056; UER00094.
Family and domain databases
Gene3D	3.30.230.10. 1 hit.
HAMAP	MF_00061. IspE.
InterPro	IPR006204. GHMP_kinase_N_dom. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
PANTHER	PTHR20861:SF2. PTHR20861:SF2. 1 hit.
Pfam	PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. ispE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_FERNB

Accession

Primary (citable) accession number: A7HNL5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	September 11, 2007
Last modified:	May 1, 2013
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program