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A7HNE8 (DDL_FERNB) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:Fnod_1588
OrganismFervidobacterium nodosum (strain ATCC 35602 / DSM 5306 / Rt17-B1) [Complete proteome] [HAMAP]
Taxonomic identifier381764 [NCBI]
Taxonomic lineageBacteriaThermotogaeThermotogalesThermotogaceaeFervidobacterium

Protein attributes

Sequence length316 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 316316D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_1000071101

Regions

Domain108 – 310203ATP-grasp
Nucleotide binding138 – 19356ATP By similarity

Sites

Metal binding2651Magnesium or manganese 1 By similarity
Metal binding2771Magnesium or manganese 1 By similarity
Metal binding2771Magnesium or manganese 2 By similarity
Metal binding2791Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
A7HNE8 [UniParc].

Last modified September 11, 2007. Version 1.
Checksum: 9B148F775030171D

FASTA31635,748
        10         20         30         40         50         60 
MYLGVLLGGI SREREISIRS GKRIAQALRN MGHVVDEIDV DDNFIYKLSE LKKYDALFNI 

        70         80         90        100        110        120 
LHGTFGEDGK MQAILDSIGI PYTGSGVETS VIAFDKYLCN LFVENTIERY EELSVVKIPN 

       130        140        150        160        170        180 
FLLISSEEFE ESKIYMIEEK IGLPCVVKPR KEGSSIGTHI CFSKEELLDA LKNEFKNYDE 

       190        200        210        220        230        240 
MIVQEYIKGK EITVSVIDIN GTPTVLPILE LRPKKLFYDY EAKYTDGMTE FIIPAELDGE 

       250        260        270        280        290        300 
TTEKINHAVL KIYKSLGCKH FSRIDGIVKD GVFYFLEVNT LPGMTELSDL PMSANAFGIS 

       310 
FDELVDLIIK EACRKL 

« Hide

References

[1]"Complete sequence of Fervidobacterium nodosum Rt17-B1."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. expand/collapse author list , Mikhailova N., Nelson K., Gogarten J.P., Noll K., Richardson P.
Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 35602 / DSM 5306 / Rt17-B1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000771 Genomic DNA. Translation: ABS61431.1.
RefSeqYP_001411088.1. NC_009718.1.

3D structure databases

ProteinModelPortalA7HNE8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING381764.Fnod_1588.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABS61431; ABS61431; Fnod_1588.
GeneID5451664.
KEGGfno:Fnod_1588.
PATRIC21884747. VBIFerNod12464_1651.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OrthoDBEOG6ND0KB.

Enzyme and pathway databases

BioCycFNOD381764:GC5M-1644-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_FERNB
AccessionPrimary (citable) accession number: A7HNE8
Entry history
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: September 11, 2007
Last modified: May 14, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways