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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Fervidobacterium nodosum (strain ATCC 35602 / DSM 5306 / Rt17-B1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei581 – 5811ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciFNOD381764:GC5M-1574-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Fnod_1522
OrganismiFervidobacterium nodosum (strain ATCC 35602 / DSM 5306 / Rt17-B1)
Taxonomic identifieri381764 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeFervidobacterium
ProteomesiUP000002415: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 819819Leucine--tRNA ligasePRO_1000071110Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi381764.Fnod_1522.

Structurei

3D structure databases

ProteinModelPortaliA7HN82.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi41 – 5111"HIGH" regionAdd
BLAST
Motifi578 – 5825"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A7HN82-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKEYKPQEIE SKWQGVWSEK KVFETPQYSD KKKYYALVMF PYPSGTLHVG
60 70 80 90 100
HVKNYVIGDI VARYKRMQGY NVLHPFGYDA FGLPAENAAI AHKIHPKKWT
110 120 130 140 150
LDNINVIRGQ IKKIGISYDW NREVITCTED YYKWTQWVFL KLYEAGLAYK
160 170 180 190 200
KPGAVNWCPS CQTVLANEQV KDGKCERCGT TVTMKYLEQW YFKITDYAEK
210 220 230 240 250
LLEGLDRLPG WPEHVKTMQR NWIGKSTGAE VDFPVDGMDR KIRIFTTRPD
260 270 280 290 300
TIYGVTFMAI APESPLVMEL VTEDKKKEVE EFLAKVALED RFKRTSVEAK
310 320 330 340 350
KEGVFLGRYA INPLTNEKIP IYVANYILYE YGTGAIMAVP AHDQRDYDFA
360 370 380 390 400
KTYNLPIKQV IKPKDGEWNI NERPYEEEGI MINSGPFDGL ESSKGIEEVT
410 420 430 440 450
KYIEEKGFGK KSVQYKLRDW LISRQRYWGA PIPIVYCEKC GIVPVPEKDL
460 470 480 490 500
PVRLPENVEF LPTGQSPLTL SEEFKHTTCP KCGGPAHREV ETMDTFVDSS
510 520 530 540 550
WYFLRYVNPK LDDKPFESDD VNYWLPVDQY IGGVEHAVLH LLYSRFITKV
560 570 580 590 600
LHDLGYLKFD EPFENLFTQG MIYKDGWKMS KSKGNVVSPD DMINKYGADT
610 620 630 640 650
LRMYILFMAP PEKDAEWNDA GIDGVNRFIK RLWNNYYKIL DIINSNDTKN
660 670 680 690 700
ENEFGKEEKN LRRKLHAMIK KIKEDIEGGF KFNTAIAGLM EFNNQLSDYL
710 720 730 740 750
ENTKSPNKKL LREIAEKVVL ILSPFAPHMA EEMWHDLGKE TLIVEEKWPE
760 770 780 790 800
YDPEALKEDE LTIVVQVNGK VRGKITVPAD ASEEEIKNRA VENAGKFLEG
810
KTIVNTIYVK GKLVNIVIK
Length:819
Mass (Da):94,678
Last modified:September 11, 2007 - v1
Checksum:iFB35036960663EDB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000771 Genomic DNA. Translation: ABS61365.1.
RefSeqiYP_001411022.1. NC_009718.1.

Genome annotation databases

EnsemblBacteriaiABS61365; ABS61365; Fnod_1522.
GeneIDi5451965.
KEGGifno:Fnod_1522.
PATRICi21884601. VBIFerNod12464_1582.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000771 Genomic DNA. Translation: ABS61365.1.
RefSeqiYP_001411022.1. NC_009718.1.

3D structure databases

ProteinModelPortaliA7HN82.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi381764.Fnod_1522.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABS61365; ABS61365; Fnod_1522.
GeneIDi5451965.
KEGGifno:Fnod_1522.
PATRICi21884601. VBIFerNod12464_1582.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciFNOD381764:GC5M-1574-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35602 / DSM 5306 / Rt17-B1.

Entry informationi

Entry nameiSYL_FERNB
AccessioniPrimary (citable) accession number: A7HN82
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: September 11, 2007
Last modified: January 7, 2015
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.