Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

A7HN82 (SYL_FERNB) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Fnod_1522
OrganismFervidobacterium nodosum (strain ATCC 35602 / DSM 5306 / Rt17-B1) [Complete proteome] [HAMAP]
Taxonomic identifier381764 [NCBI]
Taxonomic lineageBacteriaThermotogaeThermotogalesThermotogaceaeFervidobacterium

Protein attributes

Sequence length819 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 819819Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000071110

Regions

Motif41 – 5111"HIGH" region HAMAP-Rule MF_00049
Motif578 – 5825"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5811ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A7HN82 [UniParc].

Last modified September 11, 2007. Version 1.
Checksum: FB35036960663EDB

FASTA81994,678
        10         20         30         40         50         60 
MKEYKPQEIE SKWQGVWSEK KVFETPQYSD KKKYYALVMF PYPSGTLHVG HVKNYVIGDI 

        70         80         90        100        110        120 
VARYKRMQGY NVLHPFGYDA FGLPAENAAI AHKIHPKKWT LDNINVIRGQ IKKIGISYDW 

       130        140        150        160        170        180 
NREVITCTED YYKWTQWVFL KLYEAGLAYK KPGAVNWCPS CQTVLANEQV KDGKCERCGT 

       190        200        210        220        230        240 
TVTMKYLEQW YFKITDYAEK LLEGLDRLPG WPEHVKTMQR NWIGKSTGAE VDFPVDGMDR 

       250        260        270        280        290        300 
KIRIFTTRPD TIYGVTFMAI APESPLVMEL VTEDKKKEVE EFLAKVALED RFKRTSVEAK 

       310        320        330        340        350        360 
KEGVFLGRYA INPLTNEKIP IYVANYILYE YGTGAIMAVP AHDQRDYDFA KTYNLPIKQV 

       370        380        390        400        410        420 
IKPKDGEWNI NERPYEEEGI MINSGPFDGL ESSKGIEEVT KYIEEKGFGK KSVQYKLRDW 

       430        440        450        460        470        480 
LISRQRYWGA PIPIVYCEKC GIVPVPEKDL PVRLPENVEF LPTGQSPLTL SEEFKHTTCP 

       490        500        510        520        530        540 
KCGGPAHREV ETMDTFVDSS WYFLRYVNPK LDDKPFESDD VNYWLPVDQY IGGVEHAVLH 

       550        560        570        580        590        600 
LLYSRFITKV LHDLGYLKFD EPFENLFTQG MIYKDGWKMS KSKGNVVSPD DMINKYGADT 

       610        620        630        640        650        660 
LRMYILFMAP PEKDAEWNDA GIDGVNRFIK RLWNNYYKIL DIINSNDTKN ENEFGKEEKN 

       670        680        690        700        710        720 
LRRKLHAMIK KIKEDIEGGF KFNTAIAGLM EFNNQLSDYL ENTKSPNKKL LREIAEKVVL 

       730        740        750        760        770        780 
ILSPFAPHMA EEMWHDLGKE TLIVEEKWPE YDPEALKEDE LTIVVQVNGK VRGKITVPAD 

       790        800        810 
ASEEEIKNRA VENAGKFLEG KTIVNTIYVK GKLVNIVIK 

« Hide

References

[1]"Complete sequence of Fervidobacterium nodosum Rt17-B1."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. expand/collapse author list , Mikhailova N., Nelson K., Gogarten J.P., Noll K., Richardson P.
Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 35602 / DSM 5306 / Rt17-B1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000771 Genomic DNA. Translation: ABS61365.1.
RefSeqYP_001411022.1. NC_009718.1.

3D structure databases

ProteinModelPortalA7HN82.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING381764.Fnod_1522.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABS61365; ABS61365; Fnod_1522.
GeneID5451965.
KEGGfno:Fnod_1522.
PATRIC21884601. VBIFerNod12464_1582.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAERYWMNV.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycFNOD381764:GC5M-1574-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_FERNB
AccessionPrimary (citable) accession number: A7HN82
Entry history
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: September 11, 2007
Last modified: May 14, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries