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A7HMY6 (SYI_FERNB) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Isoleucine--tRNA ligase
EC=6.1.1.5
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS
Gene names
Name:ileS
Ordered Locus Names:Fnod_1425
OrganismFervidobacterium nodosum (strain ATCC 35602 / DSM 5306 / Rt17-B1) [Complete proteome] [HAMAP]
Taxonomic identifier381764 [NCBI]
Taxonomic lineageBacteria › Thermotogae › Thermotogales › Thermotogaceae › Fervidobacterium ›

Protein attributes

Sequence length913 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP-Rule MF_02002

Catalytic activity

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP-Rule MF_02002

Cofactor

Binds 1 zinc ion per subunit By similarity.

Subunit structure

Monomer By similarity.

Subcellular location

Cytoplasm By similarity.

Domain

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP-Rule MF_02002

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 913913Isoleucine--tRNA ligase HAMAP-Rule MF_02002
PRO_1000073709

Regions

Motif57 – 6711"HIGH" region HAMAP-Rule MF_02002
Motif590 – 5945"KMSKS" region HAMAP-Rule MF_02002

Sites

Metal binding8811Zinc By similarity
Metal binding8841Zinc By similarity
Metal binding9011Zinc By similarity
Metal binding9041Zinc By similarity
Binding site5491Aminoacyl-adenylate By similarity
Binding site5931ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A7HMY6 [UniParc].

Last modified September 11, 2007. Version 1.
Checksum: 0EA10A0A9A59CE16

FASTA913105,673
        10         20         30         40         50         60 
MDYKETLNLP QTNFQMKANL VQKEPNMLKY WEEKEIYKKT LETREGAPTY LLHDGPPYAN 

        70         80         90        100        110        120 
GDIHLGTAMN KILKDFVTRY KTMRGYRVPY VPGWDTHGLP IEHRVTTTLG EEAKKKSPVE 

       130        140        150        160        170        180 
IRKLCKEFAL KYVDIQREEF KRLGVKGDWE HPYITLTPDY EYHILDVFKT LVENGNVYRG 

       190        200        210        220        230        240 
NKPVYWCPTC RTALAEAEIE YHDHESPSIY VKFQMVDQPD TYVVIWTTTP WTIPANVAIA 

       250        260        270        280        290        300 
LHPEYEYLKI KVGNEYWIVA DGLLQKFASE TGIEFQVTEK FLGKTLEGKL TKHPLYDRTS 

       310        320        330        340        350        360 
VIVLADYVTL EDGTGCVHTA PGHGEEDYQT GLKYNLPILS PVDDEGKFTK EAGKYEGLKI 

       370        380        390        400        410        420 
WDANKVVVED LEKAGALIKF GKISHSYPHC WRCKGPVMFR ATPQWFISVN KNNLRGKVLE 

       430        440        450        460        470        480 
QIKKVKWYPS WGENRITAMV QERPDWTISR QRVWGVPIPA VKCKSCDEVT LDPKVIEHFA 

       490        500        510        520        530        540 
NIVKEKGTDA WFELDIKELI PSDFSCPKCG SKEFEKTYDT LDVWIDSGCS WEAVIRSKGE 

       550        560        570        580        590        600 
KFPVDLYLEG DDQHRGWFQS SIFLATAKTG NAPFKTVVTH GFIKDEQGRK MSKSLGNVID 

       610        620        630        640        650        660 
PMEIVNKYGA DILRLWVAST DFFDNIRVGK NIIEQQVEVY RKLRNTLRYL LSNLYDFTEN 

       670        680        690        700        710        720 
DILPYEKLLP LDKWALGRLQ KYIEQVTQYY EEFEYSKVYN ATVKYCTTEL SSIYLDILKD 

       730        740        750        760        770        780 
RLYVEAKDSI YRRSAQTVLY YILEALIKIL APIMPFTAEE AYQESPLKKY ESVHLENWPE 

       790        800        810        820        830        840 
VRKEFINENL LEEFQQLLLV RDDVLKALEN ARSSDVIGHS LDAHVKIEPK NSEIGQLLEK 

       850        860        870        880        890        900 
YADMLEDFFI VSKVSILNEL SDGINGQLVT VLVEHAEGQK CQRCWKYHPD TGKDENHPET 

       910 
CPRCSAVLRG ERK

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References

[1]"Complete sequence of Fervidobacterium nodosum Rt17-B1."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. expand/collapse author list , Mikhailova N., Nelson K., Gogarten J.P., Noll K., Richardson P.
Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 35602 / DSM 5306 / Rt17-B1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000771 Genomic DNA. Translation: ABS61269.1.
RefSeqYP_001410926.1. NC_009718.1.

3D structure databases

ProteinModelPortalA7HMY6.
ModBaseSearch...

Protein-protein interaction databases

STRING381764.Fnod_1425.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABS61269; ABS61269; Fnod_1425.
GeneID5452580.
KEGGfno:Fnod_1425.
PATRIC21884399. VBIFerNod12464_1482.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0060.
HOGENOMHOG000246402.
KOK01870.
OMAKQVLTHG.
ProtClustDBPRK05743.

Family and domain databases

Gene3D3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_02002. Ile_tRNA_synth_type1.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSPR00984. TRNASYNTHILE.
SUPFAMSSF47323. tRNAsyn_1a_bind. 1 hit.
SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMsTIGR00392. ileS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYI_FERNB
AccessionPrimary (citable) accession number: A7HMY6
Entry history
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: September 11, 2007
Last modified: May 1, 2013
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

SIMILARITY comments

Index of protein domains and families

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