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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Fervidobacterium nodosum (strain ATCC 35602 / DSM 5306 / Rt17-B1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei549Aminoacyl-adenylateUniRule annotation1
Binding sitei593ATPUniRule annotation1
Metal bindingi881ZincUniRule annotation1
Metal bindingi884ZincUniRule annotation1
Metal bindingi901ZincUniRule annotation1
Metal bindingi904ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciFNOD381764:G1G9K-1471-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Fnod_1425
OrganismiFervidobacterium nodosum (strain ATCC 35602 / DSM 5306 / Rt17-B1)
Taxonomic identifieri381764 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesFervidobacteriaceaeFervidobacterium
Proteomesi
  • UP000002415 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000737091 – 913Isoleucine--tRNA ligaseAdd BLAST913

Proteomic databases

PRIDEiA7HMY6

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi381764.Fnod_1425

Structurei

3D structure databases

ProteinModelPortaliA7HMY6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi57 – 67"HIGH" regionAdd BLAST11
Motifi590 – 594"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246402
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

A7HMY6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYKETLNLP QTNFQMKANL VQKEPNMLKY WEEKEIYKKT LETREGAPTY
60 70 80 90 100
LLHDGPPYAN GDIHLGTAMN KILKDFVTRY KTMRGYRVPY VPGWDTHGLP
110 120 130 140 150
IEHRVTTTLG EEAKKKSPVE IRKLCKEFAL KYVDIQREEF KRLGVKGDWE
160 170 180 190 200
HPYITLTPDY EYHILDVFKT LVENGNVYRG NKPVYWCPTC RTALAEAEIE
210 220 230 240 250
YHDHESPSIY VKFQMVDQPD TYVVIWTTTP WTIPANVAIA LHPEYEYLKI
260 270 280 290 300
KVGNEYWIVA DGLLQKFASE TGIEFQVTEK FLGKTLEGKL TKHPLYDRTS
310 320 330 340 350
VIVLADYVTL EDGTGCVHTA PGHGEEDYQT GLKYNLPILS PVDDEGKFTK
360 370 380 390 400
EAGKYEGLKI WDANKVVVED LEKAGALIKF GKISHSYPHC WRCKGPVMFR
410 420 430 440 450
ATPQWFISVN KNNLRGKVLE QIKKVKWYPS WGENRITAMV QERPDWTISR
460 470 480 490 500
QRVWGVPIPA VKCKSCDEVT LDPKVIEHFA NIVKEKGTDA WFELDIKELI
510 520 530 540 550
PSDFSCPKCG SKEFEKTYDT LDVWIDSGCS WEAVIRSKGE KFPVDLYLEG
560 570 580 590 600
DDQHRGWFQS SIFLATAKTG NAPFKTVVTH GFIKDEQGRK MSKSLGNVID
610 620 630 640 650
PMEIVNKYGA DILRLWVAST DFFDNIRVGK NIIEQQVEVY RKLRNTLRYL
660 670 680 690 700
LSNLYDFTEN DILPYEKLLP LDKWALGRLQ KYIEQVTQYY EEFEYSKVYN
710 720 730 740 750
ATVKYCTTEL SSIYLDILKD RLYVEAKDSI YRRSAQTVLY YILEALIKIL
760 770 780 790 800
APIMPFTAEE AYQESPLKKY ESVHLENWPE VRKEFINENL LEEFQQLLLV
810 820 830 840 850
RDDVLKALEN ARSSDVIGHS LDAHVKIEPK NSEIGQLLEK YADMLEDFFI
860 870 880 890 900
VSKVSILNEL SDGINGQLVT VLVEHAEGQK CQRCWKYHPD TGKDENHPET
910
CPRCSAVLRG ERK
Length:913
Mass (Da):105,673
Last modified:September 11, 2007 - v1
Checksum:i0EA10A0A9A59CE16
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000771 Genomic DNA Translation: ABS61269.1
RefSeqiWP_011994575.1, NC_009718.1

Genome annotation databases

EnsemblBacteriaiABS61269; ABS61269; Fnod_1425
KEGGifno:Fnod_1425

Similar proteinsi

Entry informationi

Entry nameiSYI_FERNB
AccessioniPrimary (citable) accession number: A7HMY6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: September 11, 2007
Last modified: May 23, 2018
This is version 77 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

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