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A7HMY6

- SYI_FERNB

UniProt

A7HMY6 - SYI_FERNB

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Fervidobacterium nodosum (strain ATCC 35602 / DSM 5306 / Rt17-B1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 53 (01 Oct 2014)
      Sequence version 1 (11 Sep 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei549 – 5491Aminoacyl-adenylateUniRule annotation
    Binding sitei593 – 5931ATPUniRule annotation
    Metal bindingi881 – 8811ZincUniRule annotation
    Metal bindingi884 – 8841ZincUniRule annotation
    Metal bindingi901 – 9011ZincUniRule annotation
    Metal bindingi904 – 9041ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciFNOD381764:GC5M-1476-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:Fnod_1425
    OrganismiFervidobacterium nodosum (strain ATCC 35602 / DSM 5306 / Rt17-B1)
    Taxonomic identifieri381764 [NCBI]
    Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeFervidobacterium
    ProteomesiUP000002415: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 913913Isoleucine--tRNA ligasePRO_1000073709Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi381764.Fnod_1425.

    Structurei

    3D structure databases

    ProteinModelPortaliA7HMY6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi57 – 6711"HIGH" regionAdd
    BLAST
    Motifi590 – 5945"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKESHFDE.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A7HMY6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDYKETLNLP QTNFQMKANL VQKEPNMLKY WEEKEIYKKT LETREGAPTY    50
    LLHDGPPYAN GDIHLGTAMN KILKDFVTRY KTMRGYRVPY VPGWDTHGLP 100
    IEHRVTTTLG EEAKKKSPVE IRKLCKEFAL KYVDIQREEF KRLGVKGDWE 150
    HPYITLTPDY EYHILDVFKT LVENGNVYRG NKPVYWCPTC RTALAEAEIE 200
    YHDHESPSIY VKFQMVDQPD TYVVIWTTTP WTIPANVAIA LHPEYEYLKI 250
    KVGNEYWIVA DGLLQKFASE TGIEFQVTEK FLGKTLEGKL TKHPLYDRTS 300
    VIVLADYVTL EDGTGCVHTA PGHGEEDYQT GLKYNLPILS PVDDEGKFTK 350
    EAGKYEGLKI WDANKVVVED LEKAGALIKF GKISHSYPHC WRCKGPVMFR 400
    ATPQWFISVN KNNLRGKVLE QIKKVKWYPS WGENRITAMV QERPDWTISR 450
    QRVWGVPIPA VKCKSCDEVT LDPKVIEHFA NIVKEKGTDA WFELDIKELI 500
    PSDFSCPKCG SKEFEKTYDT LDVWIDSGCS WEAVIRSKGE KFPVDLYLEG 550
    DDQHRGWFQS SIFLATAKTG NAPFKTVVTH GFIKDEQGRK MSKSLGNVID 600
    PMEIVNKYGA DILRLWVAST DFFDNIRVGK NIIEQQVEVY RKLRNTLRYL 650
    LSNLYDFTEN DILPYEKLLP LDKWALGRLQ KYIEQVTQYY EEFEYSKVYN 700
    ATVKYCTTEL SSIYLDILKD RLYVEAKDSI YRRSAQTVLY YILEALIKIL 750
    APIMPFTAEE AYQESPLKKY ESVHLENWPE VRKEFINENL LEEFQQLLLV 800
    RDDVLKALEN ARSSDVIGHS LDAHVKIEPK NSEIGQLLEK YADMLEDFFI 850
    VSKVSILNEL SDGINGQLVT VLVEHAEGQK CQRCWKYHPD TGKDENHPET 900
    CPRCSAVLRG ERK 913
    Length:913
    Mass (Da):105,673
    Last modified:September 11, 2007 - v1
    Checksum:i0EA10A0A9A59CE16
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000771 Genomic DNA. Translation: ABS61269.1.
    RefSeqiWP_011994575.1. NC_009718.1.
    YP_001410926.1. NC_009718.1.

    Genome annotation databases

    EnsemblBacteriaiABS61269; ABS61269; Fnod_1425.
    GeneIDi5452580.
    KEGGifno:Fnod_1425.
    PATRICi21884399. VBIFerNod12464_1482.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000771 Genomic DNA. Translation: ABS61269.1 .
    RefSeqi WP_011994575.1. NC_009718.1.
    YP_001410926.1. NC_009718.1.

    3D structure databases

    ProteinModelPortali A7HMY6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 381764.Fnod_1425.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABS61269 ; ABS61269 ; Fnod_1425 .
    GeneIDi 5452580.
    KEGGi fno:Fnod_1425.
    PATRICi 21884399. VBIFerNod12464_1482.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KESHFDE.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci FNOD381764:GC5M-1476-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 35602 / DSM 5306 / Rt17-B1.

    Entry informationi

    Entry nameiSYI_FERNB
    AccessioniPrimary (citable) accession number: A7HMY6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 26, 2008
    Last sequence update: September 11, 2007
    Last modified: October 1, 2014
    This is version 53 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3