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A7HMY6

- SYI_FERNB

UniProt

A7HMY6 - SYI_FERNB

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Protein
Isoleucine--tRNA ligase
Gene
ileS, Fnod_1425
Organism
Fervidobacterium nodosum (strain ATCC 35602 / DSM 5306 / Rt17-B1)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei549 – 5491Aminoacyl-adenylate By similarity
Binding sitei593 – 5931ATP By similarity
Metal bindingi881 – 8811Zinc By similarity
Metal bindingi884 – 8841Zinc By similarity
Metal bindingi901 – 9011Zinc By similarity
Metal bindingi904 – 9041Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciFNOD381764:GC5M-1476-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:Fnod_1425
OrganismiFervidobacterium nodosum (strain ATCC 35602 / DSM 5306 / Rt17-B1)
Taxonomic identifieri381764 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeFervidobacterium
ProteomesiUP000002415: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 913913Isoleucine--tRNA ligaseUniRule annotation
PRO_1000073709Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi381764.Fnod_1425.

Structurei

3D structure databases

ProteinModelPortaliA7HMY6.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionUniRule annotation
Add
BLAST
Motifi590 – 5945"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKESHFDE.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A7HMY6-1 [UniParc]FASTAAdd to Basket

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MDYKETLNLP QTNFQMKANL VQKEPNMLKY WEEKEIYKKT LETREGAPTY    50
LLHDGPPYAN GDIHLGTAMN KILKDFVTRY KTMRGYRVPY VPGWDTHGLP 100
IEHRVTTTLG EEAKKKSPVE IRKLCKEFAL KYVDIQREEF KRLGVKGDWE 150
HPYITLTPDY EYHILDVFKT LVENGNVYRG NKPVYWCPTC RTALAEAEIE 200
YHDHESPSIY VKFQMVDQPD TYVVIWTTTP WTIPANVAIA LHPEYEYLKI 250
KVGNEYWIVA DGLLQKFASE TGIEFQVTEK FLGKTLEGKL TKHPLYDRTS 300
VIVLADYVTL EDGTGCVHTA PGHGEEDYQT GLKYNLPILS PVDDEGKFTK 350
EAGKYEGLKI WDANKVVVED LEKAGALIKF GKISHSYPHC WRCKGPVMFR 400
ATPQWFISVN KNNLRGKVLE QIKKVKWYPS WGENRITAMV QERPDWTISR 450
QRVWGVPIPA VKCKSCDEVT LDPKVIEHFA NIVKEKGTDA WFELDIKELI 500
PSDFSCPKCG SKEFEKTYDT LDVWIDSGCS WEAVIRSKGE KFPVDLYLEG 550
DDQHRGWFQS SIFLATAKTG NAPFKTVVTH GFIKDEQGRK MSKSLGNVID 600
PMEIVNKYGA DILRLWVAST DFFDNIRVGK NIIEQQVEVY RKLRNTLRYL 650
LSNLYDFTEN DILPYEKLLP LDKWALGRLQ KYIEQVTQYY EEFEYSKVYN 700
ATVKYCTTEL SSIYLDILKD RLYVEAKDSI YRRSAQTVLY YILEALIKIL 750
APIMPFTAEE AYQESPLKKY ESVHLENWPE VRKEFINENL LEEFQQLLLV 800
RDDVLKALEN ARSSDVIGHS LDAHVKIEPK NSEIGQLLEK YADMLEDFFI 850
VSKVSILNEL SDGINGQLVT VLVEHAEGQK CQRCWKYHPD TGKDENHPET 900
CPRCSAVLRG ERK 913
Length:913
Mass (Da):105,673
Last modified:September 11, 2007 - v1
Checksum:i0EA10A0A9A59CE16
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000771 Genomic DNA. Translation: ABS61269.1.
RefSeqiWP_011994575.1. NC_009718.1.
YP_001410926.1. NC_009718.1.

Genome annotation databases

EnsemblBacteriaiABS61269; ABS61269; Fnod_1425.
GeneIDi5452580.
KEGGifno:Fnod_1425.
PATRICi21884399. VBIFerNod12464_1482.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000771 Genomic DNA. Translation: ABS61269.1 .
RefSeqi WP_011994575.1. NC_009718.1.
YP_001410926.1. NC_009718.1.

3D structure databases

ProteinModelPortali A7HMY6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 381764.Fnod_1425.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABS61269 ; ABS61269 ; Fnod_1425 .
GeneIDi 5452580.
KEGGi fno:Fnod_1425.
PATRICi 21884399. VBIFerNod12464_1482.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KESHFDE.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci FNOD381764:GC5M-1476-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35602 / DSM 5306 / Rt17-B1.

Entry informationi

Entry nameiSYI_FERNB
AccessioniPrimary (citable) accession number: A7HMY6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: September 11, 2007
Last modified: September 3, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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