A7HLE7 (A7HLE7_FERNB) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase HAMAP MF_00193
EC=6.3.1.5 HAMAP MF_00193

Gene names

Name:	nadE HAMAP MF_00193
Ordered Locus Names:	Fnod_0878

Organism

Fervidobacterium nodosum (strain ATCC 35602 / DSM 5306 / Rt17-B1) [Complete proteome] [HAMAP] EMBL ABS60730.1

Taxonomic identifier

381764 [NCBI]

Taxonomic lineage

Bacteria › Thermotogae › Thermotogales › Thermotogaceae › Fervidobacterium

Protein attributes

Sequence length	294 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193 SAAS SAAS003694
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193 SAAS SAAS003694
Sequence similarities	Belongs to the NAD synthetase family. HAMAP MF_00193

Ontologies

Keywords
Ligand	ATP-binding HAMAP MF_00193 SAAS SAAS003694 NAD HAMAP MF_00193 SAAS SAAS003694 Nucleotide-binding
Molecular function	Ligase SAAS SAAS003694 HAMAP MF_00193
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	31 – 38	8	ATP By similarity HAMAP MF_00193
Sites
	Active site	33	1	By similarity HAMAP MF_00193

Sequences

	Sequence		Length	Mass (Da)
	A7HLE7 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: 77A2C74519E0C8AE	FASTA	294	33,841
	10 20 30 40 50 60 MIFNPKDEII RIEKFIKRTM ETYNFKGAVI GISGGVDSAV VLALLTRTIE KENILALILP 70 80 90 100 110 120 ERDSSKESVR DAKLVCNYFG VNYKIFPITG ALRKLGAYSL FPPALFIPES IKISYSKKRW 130 140 150 160 170 180 ERYQDPYIMD LKNEGDELFL KGLAYYRIKH RVRMCKLYME AEKRGYCVVG TTNKTELALG 190 200 210 220 230 240 LYVKWGDDAV DIEPIKHLYK TQVFELAKHL GVPEKIINKK PTPDLIPGIN DEDAFGIEYS 250 260 270 280 290 KLDKILFSIE SNEGKVEDID EKLLSKVKEI MRFTKIRELK GLGIDDISNQ KDRW

References

[1]

"Complete sequence of Fervidobacterium nodosum Rt17-B1." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Mikhailova N., Nelson K., Gogarten J.P., Noll K., Richardson P. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35602 / DSM 5306 / Rt17-B1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000771 Genomic DNA. Translation: ABS60730.1.
RefSeq	YP_001410387.1. NC_009718.1.
3D structure databases
ProteinModelPortal	A7HLE7.
ModBase	Search...
Protein-protein interaction databases
STRING	A7HLE7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5451990.
GenomeReviews	Gene locus Fnod_0878 in contig CP000771_GR.
KEGG	fno:Fnod_0878.
PATRIC	21883239. VBIFerNod12464_0920.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0171.
HOGENOM	HBG351567.
OMA	YDISARD.
ProtClustDB	CLSK875266.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K01916.
Pfam	PF02540. NAD_synthase. 2 hits. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A7HLE7_FERNB

Accession

Primary (citable) accession number: A7HLE7

Entry history

Integrated into UniProtKB/TrEMBL:	September 11, 2007
Last sequence update:	September 11, 2007
Last modified:	December 14, 2011
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)