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A7HKV0 (SYE1_FERNB) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate--tRNA ligase 1

EC=6.1.1.17
Alternative name(s):
Glutamyl-tRNA synthetase 1
Short name=GluRS 1
Gene names
Name:gltX1
Ordered Locus Names:Fnod_0678
OrganismFervidobacterium nodosum (strain ATCC 35602 / DSM 5306 / Rt17-B1) [Complete proteome] [HAMAP]
Taxonomic identifier381764 [NCBI]
Taxonomic lineageBacteriaThermotogaeThermotogalesThermotogaceaeFervidobacterium

Protein attributes

Sequence length469 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP-Rule MF_00022

Catalytic activity

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP-Rule MF_00022

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00022

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00022.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglutamyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glutamate-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 469469Glutamate--tRNA ligase 1 HAMAP-Rule MF_00022
PRO_0000367672

Regions

Motif10 – 2011"HIGH" region HAMAP-Rule MF_00022
Motif252 – 2565"KMSKS" region HAMAP-Rule MF_00022

Sites

Binding site2551ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A7HKV0 [UniParc].

Last modified September 11, 2007. Version 1.
Checksum: 5315499B19B106C0

FASTA46953,949
        10         20         30         40         50         60 
MSEVRVRFAP SPTGYLHVGG ARTALFNYLY ARKTGGKFIL RIEDTDLERS EKVFEEQLIS 

        70         80         90        100        110        120 
ALKWLGLEWD EGPDIGGQYG PYRQSERVHL YHEYAQKLIE EGKAYEVYAY PEEIEQLREK 

       130        140        150        160        170        180 
LLSEGKAPHY TREMLEPYNT PERKKEYEEK GLKPAVYFSM LRKDYVINDV VKGEVVFKAG 

       190        200        210        220        230        240 
SVGDFALLRS NGMPTYNYAC VIDDGLMKIT HVLRGDDHLS NTVKQVALYE AFGWPTPVFG 

       250        260        270        280        290        300 
HVSMILGPDG SKLSKRHGAT SVEEFKSRGY LPEALVNFLA LLGWSHPEGK EILTKQELIE 

       310        320        330        340        350        360 
SFSLERLVKN PAIFNPEKLK WMNSEHIRMK SMDELVKIAK PFLQKDVDDE YFAKILHAVK 

       370        380        390        400        410        420 
DRIEELSQLP ELTDIFFEKP HQLPEKTPEA IETYTNLLSE LKKIEKWDKD SIYAAFKTAM 

       430        440        450        460 
KSAKLKGKDF YMTLRLVLTG KTEGPELIDI LEILGKDEVI ERISLYLNK 

« Hide

References

[1]"Complete sequence of Fervidobacterium nodosum Rt17-B1."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. expand/collapse author list , Mikhailova N., Nelson K., Gogarten J.P., Noll K., Richardson P.
Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 35602 / DSM 5306 / Rt17-B1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000771 Genomic DNA. Translation: ABS60533.1.
RefSeqYP_001410190.1. NC_009718.1.

3D structure databases

ProteinModelPortalA7HKV0.
SMRA7HKV0. Positions 4-463.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING381764.Fnod_0678.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABS60533; ABS60533; Fnod_0678.
GeneID5452759.
KEGGfno:Fnod_0678.
PATRIC21882801. VBIFerNod12464_0716.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0008.
HOGENOMHOG000252720.
KOK09698.
OMAGPELIDI.
OrthoDBEOG6DRPF7.

Enzyme and pathway databases

BioCycFNOD381764:GC5M-691-MONOMER.

Family and domain databases

Gene3D1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
1.10.8.70. 1 hit.
3.40.50.620. 2 hits.
HAMAPMF_00022_B. Glu_tRNA_synth_B.
InterProIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020752. aa-tRNA-synth_I_codon-bd_sub1.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10119. PTHR10119. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF48163. SSF48163. 1 hit.
TIGRFAMsTIGR00464. gltX_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYE1_FERNB
AccessionPrimary (citable) accession number: A7HKV0
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: September 11, 2007
Last modified: May 14, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries