Reviewed, UniProtKB/Swiss-Prot A7HKV0 (SYE1_FERNB)

Names and origin

Protein names

Recommended name:
    Glutamyl-tRNA synthetase 1
    EC=6.1.1.17
Alternative name(s):
    Glutamate--tRNA ligase 1
      Short name=GluRS 1

Gene names

Name:	gltX1
Ordered Locus Names:	Fnod_0678

Organism

Fervidobacterium nodosum (strain ATCC 35602 / DSM 5306 / Rt17-B1) [Complete proteome] [HAMAP]

Taxonomic identifier

381764 [NCBI]

Taxonomic lineage

Bacteria › Thermotogae › Thermotogales › Thermotogaceae › Fervidobacterium

Protein attributes

Sequence length	469 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022
Subunit structure	Monomer By similarity. HAMAP MF_00022
Subcellular location	Cytoplasm By similarity HAMAP MF_00022.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 469	469	Glutamyl-tRNA synthetase 1 HAMAP MF_00022		PRO_0000367672
Regions
	Motif	10 – 20	11	"HIGH" region HAMAP MF_00022
	Motif	252 – 256	5	"KMSKS" region HAMAP MF_00022
Sites
	Binding site	255	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7HKV0-1 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: 5315499B19B106C0	FASTA	469	53,949
	10 20 30 40 50 60 MSEVRVRFAP SPTGYLHVGG ARTALFNYLY ARKTGGKFIL RIEDTDLERS EKVFEEQLIS 70 80 90 100 110 120 ALKWLGLEWD EGPDIGGQYG PYRQSERVHL YHEYAQKLIE EGKAYEVYAY PEEIEQLREK 130 140 150 160 170 180 LLSEGKAPHY TREMLEPYNT PERKKEYEEK GLKPAVYFSM LRKDYVINDV VKGEVVFKAG 190 200 210 220 230 240 SVGDFALLRS NGMPTYNYAC VIDDGLMKIT HVLRGDDHLS NTVKQVALYE AFGWPTPVFG 250 260 270 280 290 300 HVSMILGPDG SKLSKRHGAT SVEEFKSRGY LPEALVNFLA LLGWSHPEGK EILTKQELIE 310 320 330 340 350 360 SFSLERLVKN PAIFNPEKLK WMNSEHIRMK SMDELVKIAK PFLQKDVDDE YFAKILHAVK 370 380 390 400 410 420 DRIEELSQLP ELTDIFFEKP HQLPEKTPEA IETYTNLLSE LKKIEKWDKD SIYAAFKTAM 430 440 450 460 KSAKLKGKDF YMTLRLVLTG KTEGPELIDI LEILGKDEVI ERISLYLNK

References

[1]

"Complete sequence of Fervidobacterium nodosum Rt17-B1." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Mikhailova N., Nelson K., Gogarten J.P., Noll K., Richardson P. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000771 Genomic DNA. Translation: ABS60533.1.
RefSeq	YP_001410190.1.
3D structure databases
SMR	A7HKV0. Positions 4-463.
ModBase	Search...
Protein-protein interaction databases
STRING	A7HKV0.
Genome annotation databases
GeneID	5452759.
GenomeReviews	Gene locus Fnod_0678 in contig CP000771_GR.
KEGG	fno:Fnod_0678.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HBG628189.
OMA	SMILGPD.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ic_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ic. IPR020061. Glu/Gln-tRNA-synth_Ic_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ic_cat-dom. IPR020060. Glu/Gln-tRNA-synth_Ic_N. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
TIGRFAMs	TIGR00464. gltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE1_FERNB

Accession

Primary (citable) accession number: A7HKV0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	September 11, 2007
Last modified:	February 9, 2010
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)