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A7HKC0 (UVRB_FERNB) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
UvrABC system protein B

Short name=Protein UvrB
Alternative name(s):
Excinuclease ABC subunit B
Gene names
Name:uvrB
Ordered Locus Names:Fnod_0489
OrganismFervidobacterium nodosum (strain ATCC 35602 / DSM 5306 / Rt17-B1) [Complete proteome] [HAMAP]
Taxonomic identifier381764 [NCBI]
Taxonomic lineageBacteriaThermotogaeThermotogalesThermotogaceaeFervidobacterium

Protein attributes

Sequence length661 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA2B2 complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage By similarity. HAMAP-Rule MF_00204

Subunit structure

Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex By similarity.

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00204.

Domain

The beta-hairpin motif is involved in DNA binding By similarity. HAMAP-Rule MF_00204

Sequence similarities

Belongs to the UvrB family.

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain.

Contains 1 UVR domain.

Ontologies

Keywords
   Biological processDNA damage
DNA excision
DNA repair
SOS response
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionExcision nuclease
Helicase
Hydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processSOS response

Inferred from electronic annotation. Source: UniProtKB-HAMAP

nucleotide-excision repair

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

DNA binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

excinuclease ABC activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

helicase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 661661UvrABC system protein B HAMAP-Rule MF_00204
PRO_1000099552

Regions

Domain24 – 209186Helicase ATP-binding
Domain430 – 594165Helicase C-terminal
Domain622 – 65736UVR
Nucleotide binding37 – 448ATP By similarity
Motif90 – 11324Beta-hairpin HAMAP-Rule MF_00204

Sequences

Sequence LengthMass (Da)Tools
A7HKC0 [UniParc].

Last modified September 11, 2007. Version 1.
Checksum: ADBDAB62C682D654

FASTA66176,722
        10         20         30         40         50         60 
MPYELISDYE PMGDQPQAIE SLVNGLNKGY RFQTLLGVTG SGKTFTMANV IKEVNRPVLI 

        70         80         90        100        110        120 
ISPNKTLAAQ LYSEFKAFFP NNKVEFFISY YDYYQPEAYV PTKDLYIEKS ADINDVIARM 

       130        140        150        160        170        180 
RMSAIKSIMT RRDVIVVASV SAIYACGDPR DFDTLNIKLE VGQRINLSEF VKKLVKIGYE 

       190        200        210        220        230        240 
RKEDIGLTGS FRLRGDTLEI FPSYQDEGIR IELFGDEIDR MYTFDRMNRD VIERLDRLTI 

       250        260        270        280        290        300 
YPTKEYVTTE EKIERAVKSI RAELDEQVKK LRSEGKELEA QRLWQRTMND IELLSTLGYC 

       310        320        330        340        350        360 
TGIENYSRHF DGRQPGEPPY SLLDYYDEDF IVFIDESHIT IPQLRAMYHG EMSRKKSLVE 

       370        380        390        400        410        420 
YGFRLPCAYD NRPLKFDEFM QKVNQVIFVS ATPGPYELEV SEQVVEQIIR PTGLIDPQVE 

       430        440        450        460        470        480 
VRPTRYQVDD LVNEIVQVKK RGEKALVTVL TKKTAEMLAE YLVEFNIRAL YLHSELDAIK 

       490        500        510        520        530        540 
RVEVLKKLRA GEIDVVVGVN LLREGLDLPE VSLVAILDAD TEGFLRSETT LIQIIGRTAR 

       550        560        570        580        590        600 
NENGKVIMYA DRITPAMQRA IDETNRRRKI QMEYNEKHGI KPKTIIKPLM EDIFAPFRDK 

       610        620        630        640        650        660 
EEEMYKVYED SILQMKESLS LEEYAALLEE EMYKAASELR YEDAARLRDE LFKIKEELNR 


N 

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References

[1]"Complete sequence of Fervidobacterium nodosum Rt17-B1."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. expand/collapse author list , Mikhailova N., Nelson K., Gogarten J.P., Noll K., Richardson P.
Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 35602 / DSM 5306 / Rt17-B1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000771 Genomic DNA. Translation: ABS60353.1.
RefSeqYP_001410010.1. NC_009718.1.

3D structure databases

ProteinModelPortalA7HKC0.
SMRA7HKC0. Positions 2-590.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING381764.Fnod_0489.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABS60353; ABS60353; Fnod_0489.
GeneID5453153.
KEGGfno:Fnod_0489.
PATRIC21882379. VBIFerNod12464_0510.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0556.
HOGENOMHOG000073580.
KOK03702.
OMAIDLVPAH.
OrthoDBEOG6B360R.

Enzyme and pathway databases

BioCycFNOD381764:GC5M-497-MONOMER.

Family and domain databases

Gene3D3.40.50.300. 4 hits.
4.10.860.10. 1 hit.
HAMAPMF_00204. UvrB.
InterProIPR006935. Helicase/UvrB_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR001943. UVR_dom.
IPR004807. UvrB.
IPR024759. UvrB_YAD/RRR_dom.
[Graphical view]
PANTHERPTHR24029. PTHR24029. 1 hit.
PfamPF00271. Helicase_C. 1 hit.
PF04851. ResIII. 1 hit.
PF02151. UVR. 1 hit.
PF12344. UvrB. 1 hit.
[Graphical view]
SMARTSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMSSF46600. SSF46600. 1 hit.
SSF52540. SSF52540. 3 hits.
TIGRFAMsTIGR00631. uvrb. 1 hit.
PROSITEPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50151. UVR. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameUVRB_FERNB
AccessionPrimary (citable) accession number: A7HKC0
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: September 11, 2007
Last modified: May 14, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families