Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

UvrABC system protein B

Gene

uvrB

Organism
Fervidobacterium nodosum (strain ATCC 35602 / DSM 5306 / Rt17-B1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA2B2 complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi37 – 448ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. DNA binding Source: UniProtKB-HAMAP
  3. excinuclease ABC activity Source: UniProtKB-HAMAP
  4. helicase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nucleotide-excision repair Source: UniProtKB-HAMAP
  2. SOS response Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Excision nuclease, Helicase, Hydrolase

Keywords - Biological processi

DNA damage, DNA excision, DNA repair, SOS response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciFNOD381764:GC5M-497-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
UvrABC system protein BUniRule annotation
Short name:
Protein UvrBUniRule annotation
Alternative name(s):
Excinuclease ABC subunit BUniRule annotation
Gene namesi
Name:uvrBUniRule annotation
Ordered Locus Names:Fnod_0489
OrganismiFervidobacterium nodosum (strain ATCC 35602 / DSM 5306 / Rt17-B1)
Taxonomic identifieri381764 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeFervidobacterium
ProteomesiUP000002415: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 661661UvrABC system protein BPRO_1000099552Add
BLAST

Interactioni

Subunit structurei

Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex.UniRule annotation

Protein-protein interaction databases

STRINGi381764.Fnod_0489.

Structurei

3D structure databases

ProteinModelPortaliA7HKC0.
SMRiA7HKC0. Positions 2-590.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini24 – 209186Helicase ATP-bindingUniRule annotationAdd
BLAST
Domaini430 – 594165Helicase C-terminalUniRule annotationAdd
BLAST
Domaini622 – 65736UVRUniRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi90 – 11324Beta-hairpinAdd
BLAST

Domaini

The beta-hairpin motif is involved in DNA binding.UniRule annotation

Sequence similaritiesi

Belongs to the UvrB family.UniRule annotation
Contains 1 helicase ATP-binding domain.UniRule annotation
Contains 1 helicase C-terminal domain.UniRule annotation
Contains 1 UVR domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0556.
HOGENOMiHOG000073580.
KOiK03702.
OMAiNKTLCAQ.
OrthoDBiEOG6B360R.

Family and domain databases

Gene3Di3.40.50.300. 4 hits.
4.10.860.10. 1 hit.
HAMAPiMF_00204. UvrB.
InterProiIPR006935. Helicase/UvrB_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR001943. UVR_dom.
IPR004807. UvrB.
IPR024759. UvrB_YAD/RRR_dom.
[Graphical view]
PANTHERiPTHR24029. PTHR24029. 1 hit.
PfamiPF00271. Helicase_C. 1 hit.
PF04851. ResIII. 1 hit.
PF02151. UVR. 1 hit.
PF12344. UvrB. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF46600. SSF46600. 1 hit.
SSF52540. SSF52540. 3 hits.
TIGRFAMsiTIGR00631. uvrb. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50151. UVR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A7HKC0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPYELISDYE PMGDQPQAIE SLVNGLNKGY RFQTLLGVTG SGKTFTMANV
60 70 80 90 100
IKEVNRPVLI ISPNKTLAAQ LYSEFKAFFP NNKVEFFISY YDYYQPEAYV
110 120 130 140 150
PTKDLYIEKS ADINDVIARM RMSAIKSIMT RRDVIVVASV SAIYACGDPR
160 170 180 190 200
DFDTLNIKLE VGQRINLSEF VKKLVKIGYE RKEDIGLTGS FRLRGDTLEI
210 220 230 240 250
FPSYQDEGIR IELFGDEIDR MYTFDRMNRD VIERLDRLTI YPTKEYVTTE
260 270 280 290 300
EKIERAVKSI RAELDEQVKK LRSEGKELEA QRLWQRTMND IELLSTLGYC
310 320 330 340 350
TGIENYSRHF DGRQPGEPPY SLLDYYDEDF IVFIDESHIT IPQLRAMYHG
360 370 380 390 400
EMSRKKSLVE YGFRLPCAYD NRPLKFDEFM QKVNQVIFVS ATPGPYELEV
410 420 430 440 450
SEQVVEQIIR PTGLIDPQVE VRPTRYQVDD LVNEIVQVKK RGEKALVTVL
460 470 480 490 500
TKKTAEMLAE YLVEFNIRAL YLHSELDAIK RVEVLKKLRA GEIDVVVGVN
510 520 530 540 550
LLREGLDLPE VSLVAILDAD TEGFLRSETT LIQIIGRTAR NENGKVIMYA
560 570 580 590 600
DRITPAMQRA IDETNRRRKI QMEYNEKHGI KPKTIIKPLM EDIFAPFRDK
610 620 630 640 650
EEEMYKVYED SILQMKESLS LEEYAALLEE EMYKAASELR YEDAARLRDE
660
LFKIKEELNR N
Length:661
Mass (Da):76,722
Last modified:September 11, 2007 - v1
Checksum:iADBDAB62C682D654
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000771 Genomic DNA. Translation: ABS60353.1.
RefSeqiWP_011993673.1. NC_009718.1.
YP_001410010.1. NC_009718.1.

Genome annotation databases

EnsemblBacteriaiABS60353; ABS60353; Fnod_0489.
GeneIDi5453153.
KEGGifno:Fnod_0489.
PATRICi21882379. VBIFerNod12464_0510.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000771 Genomic DNA. Translation: ABS60353.1.
RefSeqiWP_011993673.1. NC_009718.1.
YP_001410010.1. NC_009718.1.

3D structure databases

ProteinModelPortaliA7HKC0.
SMRiA7HKC0. Positions 2-590.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi381764.Fnod_0489.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABS60353; ABS60353; Fnod_0489.
GeneIDi5453153.
KEGGifno:Fnod_0489.
PATRICi21882379. VBIFerNod12464_0510.

Phylogenomic databases

eggNOGiCOG0556.
HOGENOMiHOG000073580.
KOiK03702.
OMAiNKTLCAQ.
OrthoDBiEOG6B360R.

Enzyme and pathway databases

BioCyciFNOD381764:GC5M-497-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 4 hits.
4.10.860.10. 1 hit.
HAMAPiMF_00204. UvrB.
InterProiIPR006935. Helicase/UvrB_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR001943. UVR_dom.
IPR004807. UvrB.
IPR024759. UvrB_YAD/RRR_dom.
[Graphical view]
PANTHERiPTHR24029. PTHR24029. 1 hit.
PfamiPF00271. Helicase_C. 1 hit.
PF04851. ResIII. 1 hit.
PF02151. UVR. 1 hit.
PF12344. UvrB. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF46600. SSF46600. 1 hit.
SSF52540. SSF52540. 3 hits.
TIGRFAMsiTIGR00631. uvrb. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50151. UVR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35602 / DSM 5306 / Rt17-B1.

Entry informationi

Entry nameiUVRB_FERNB
AccessioniPrimary (citable) accession number: A7HKC0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: September 11, 2007
Last modified: January 7, 2015
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.