Reviewed, UniProtKB/Swiss-Prot A7HIJ4 (PURL_ANADF)

Names and origin

Protein names

Recommended name:
    Phosphoribosylformylglycinamidine synthase 2
    EC=6.3.5.3
Alternative name(s):
    Phosphoribosylformylglycinamidine synthase II
      Short name=FGAM synthase II

Gene names

Name:	purL
Ordered Locus Names:	Anae109_4362

Organism

Anaeromyxobacter sp. (strain Fw109-5) [Complete proteome] [HAMAP]

Taxonomic identifier

404589 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Anaeromyxobacter

Protein attributes

Sequence length	767 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00420
Subunit structure	Heterodimer of two subunits, purQ and purL. HAMAP MF_00420
Subcellular location	Cytoplasm By similarity HAMAP MF_00420.
Sequence similarities	Belongs to the FGAMS family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 767	767	Phosphoribosylformylglycinamidine synthase 2 HAMAP MF_00420		PRO_1000050295
Regions
	Nucleotide binding	103 – 114	12	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	A7HIJ4-1 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: 7C671AE89BBB4255	FASTA	767	81,037
	10 20 30 40 50 60 MTEKITPEVV AQHGLTQAEY DEVKRHLGRE PNLTELGVFS VMWSEHCSYK SSRVHLKTFP 70 80 90 100 110 120 TSGPRVLQGP GENAGVVDLG DGLAAAFKME SHNHPSYIEP YQGAATGVGG ILRDVFTMGA 130 140 150 160 170 180 RPIASANSLR FGDPSHPKTA YLLEGVVAGI GGYGNCMGVP TVAGEVMFHP SYNGNCLVNA 190 200 210 220 230 240 FTLGILPADK IFLGTAAGVG NPVMYIGAKT GRDGIHGATM ASAEFDASTE EKRPTVQVGD 250 260 270 280 290 300 PFMEKLLLEA CLELFKTDAV VGIQDMGAAG FTSSSVEMAG RAGNGLDLFL DQVPLREEGM 310 320 330 340 350 360 TPYEILLSES QERMLLVAAE GKEDMVRKIC TKWDLDVAIV GRVTGTGRWR AHWHGKVVAD 370 380 390 400 410 420 LPVDPLTEGA PKYQRPMTPH PALPALHAFD LSTVPEPADL GDTLLRLLAR PTIASKEWVY 430 440 450 460 470 480 QQYDHMVRLV GAVRPGGDAA VVRIATGDER HAGKGIAISV GANGRYCFLD PHLGAQHAVA 490 500 510 520 530 540 ECARNIACVG GEPIAVTDCL NFGNPEKPEI MWQFAECVRG IGDACRALGT PVVSGNVSLY 550 560 570 580 590 600 NETEGQGILP TPTIGMVGLL PDVEKTCTSA FKNAGDVIAL VGTLQGEVGG SEYLSAEHGR 610 620 630 640 650 660 EAGHPPALDL AKEKAVQETV RRAVREGLLS SAHDCSDGGL AVALAECCMM HGVPAGAEKA 670 680 690 700 710 720 PWIGAAVRVP FPARKDFVLF GEDASRILVS MPKESAARFV ALAQECGAPV IRLGAVGGDT 730 740 750 760 LEIQGALSVP VAELAKAWRD GVPAVLRRDV GHAAASGIAR EPQEPLP

References

[1]

"Complete sequence of Anaeromyxobacter sp. Fw109-5." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Lykidis A., Fields M., Richardson P. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000769 Genomic DNA. Translation: ABS28540.1.
RefSeq	YP_001381524.1.
3D structure databases
SMR	A7HIJ4. Positions 19-695.
ModBase	Search...
Protein-protein interaction databases
STRING	A7HIJ4.
Genome annotation databases
GeneID	5376534.
GenomeReviews	Gene locus Anae109_4362 in contig CP000769_GR.
KEGG	afw:Anae109_4362.
NMPDR	fig|404589.4.peg.3734.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0046.
HOGENOM	HBG311214.
OMA	GNCVGVP.
Family and domain databases
HAMAP	MF_00420. PurL_2. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR010074. PRibForGlyAmidine_synth_II. IPR016188. PurM_N-like. [Graphical view]
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURL_ANADF

Accession

Primary (citable) accession number: A7HIJ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 11, 2007
Last modified:	February 9, 2010
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)