A7HIA1 (ILVD_ANADF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase
Short name=DAD
EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	Anae109_4269

Organism

Anaeromyxobacter sp. (strain Fw109-5) [Complete proteome] [HAMAP]

Taxonomic identifier

404589 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Anaeromyxobacter

Protein attributes

Sequence length	561 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 561	561	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_0000321590
Sites
	Metal binding	121	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	194	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	A7HIA1 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: 5558BF21AD87C006	FASTA	561	59,282
	10 20 30 40 50 60 MGVRSDTIKK GFERAPHRSL LRATGLTDAD FDKPFIGIAN SHVDIIPGHF FLQEYGRIAK 70 80 90 100 110 120 DEIRKAGGVP FEFNTIGVDD GIAMGHAGML YSLPSRELIA DSIETMMNAH SLDALLCIPN 130 140 150 160 170 180 CDKIVPGMLM GALRVDVPTV FCSGGPMKAG RMADGTAIDL TTAFEAVGKR ALGQMSDAEL 190 200 210 220 230 240 YEIECKACPS GGSCSGMFTA NSMNVLCEAM GVALPGNGTA LALTPEREAL LRRAARRAVE 250 260 270 280 290 300 IAGDERFRLR RIVNADAIHN AMVVDMAMGG SSNTVLHMLA IAREAGVEFG LRQIEAVAAK 310 320 330 340 350 360 VGHIAKIAPS LSTVHIEDVH RAGGVPAVLH EVARRGGVVR EDALTVTGET VGERIRGAEI 370 380 390 400 410 420 KDRSVIHPLE EAYSAVGGLA VLFGNLAPEG AVVKTAGIQP SMRKFTGRAI CFDSQEEAIA 430 440 450 460 470 480 GIMGGKVKPG HFVVIRYEGP RGGPGMQEML APTSLIMGMG LGEQVALATD GRFSGATRGA 490 500 510 520 530 540 CVGHVSPEAA EGGPLALVQD GDAVTIDVEA RALTVDVPDA ELARRREGFR PKRKEIRSSW 550 560 LKRYALLVSN ASAGAVMKTE L

References

[1]

"Complete sequence of Anaeromyxobacter sp. Fw109-5." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Lykidis A., Fields M., Richardson P. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Fw109-5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000769 Genomic DNA. Translation: ABS28447.1.
RefSeq	YP_001381431.1. NC_009675.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A7HIA1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5375669.
GenomeReviews	Gene locus Anae109_4269 in contig CP000769_GR.
KEGG	afw:Anae109_4269.
NMPDR	fig|404589.4.peg.3658.
PATRIC	20934067. VBIAnaSp113478_4469.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0129.
HOGENOM	HBG671001.
OMA	SQDCRWP.
ProtClustDB	PRK00911.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_ANADF

Accession

Primary (citable) accession number: A7HIA1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	September 11, 2007
Last modified:	January 25, 2012
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program