Reviewed, UniProtKB/Swiss-Prot A7HH87 (SYA_ANADF)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	Anae109_3904

Organism

Anaeromyxobacter sp. (strain Fw109-5) [Complete proteome] [HAMAP]

Taxonomic identifier

404589 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Anaeromyxobacter

Protein attributes

Sequence length	904 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036
Subcellular location	Cytoplasm By similarity HAMAP MF_00036.
Domain	The C-terminal C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity. HAMAP MF_00036
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 904	904	Alanyl-tRNA synthetase HAMAP MF_00036		PRO_0000347489

Sequences

	Sequence		Length	Mass (Da)
	A7HH87-1 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: 06D8C3289D861DC3	FASTA	904	98,558
	10 20 30 40 50 60 MKTAAQIREL FLRFFEEKAH RRVPSSSLVP QNDPTLLFTN AGMNQFKDVF TGREKRDYSR 70 80 90 100 110 120 ATTAQKCVRA GGKHNDLENV GFTARHHTFF EMLGNFSFGD YFKKEAIAWA WELVTSERWL 130 140 150 160 170 180 AISKERLAAT VFAGEGTLPW DEEAYRLWEA QGVPPERIHK LGAKDNFWAM GDTGPCGPCS 190 200 210 220 230 240 ELHFFQGNDI PCAEEKAGRT CQGVACDCDR WLEIWNLVFM QFERGADGGL TPLPKPSIDT 250 260 270 280 290 300 GAGLERMAAV VQGKRSNYDI DAFQSIIRAI EKLAGKRYGA TDADDDVSMR VIADHARATT 310 320 330 340 350 360 FLVGDGVLPA NEGRGYVLRR IMRRAIRHGK RLGLERPFLA DVCEAVMEEM GGAYPETREN 370 380 390 400 410 420 RAFIVKVAGQ EEESFRRTLD KGLAILETEM RKAIPPETHL GKPATPPPSA PRPVIDGKLA 430 440 450 460 470 480 FQLYDTFGFP LDLTRVIAAE RGFDVDEQGF DRNMAEQRAR SEWKGSGEQA VGDLHKQIAS 490 500 510 520 530 540 ELGETRFLGY EAPTARAEVK ALLANGARAA KAARGDKVEV VTAATPFYGE SGGQVGDQGS 550 560 570 580 590 600 IAAPGGRVRV EDTRRPVPGL VTHVGVVEEG ELAVGDLVEL AVDDRRRDLI RANHSATHLL 610 620 630 640 650 660 QLALRETLGD HVKQAGSIVA PDYLRFDFSH FQPLSEEELN AIERRVNELV RENAETETAV 670 680 690 700 710 720 LKLEDARQSG AMMIFGEKYG DVVRVVRLGP SKELCGGTHV RRTGDIAFFK IGSEESIAAG 730 740 750 760 770 780 VRRIVAYTGP QAIELSQREA DELRRAAALF KAGAFEVAQK IEQTQKRVKD LERALDEAKG 790 800 810 820 830 840 KIASAQSGDL AAQARDVKGA KVLAVRVQGD GKSLRELADK LRDRLGTGVV ALGAEQDGKA 850 860 870 880 890 900 ILLVAVTKDL TARLKAGELV KEAAKLVGGS GGGKPDLAQA GGSDPAGLEK ALAKVQELAV AALG

References

[1]

"Complete sequence of Anaeromyxobacter sp. Fw109-5." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Lykidis A., Fields M., Richardson P. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000769 Genomic DNA. Translation: ABS28083.1.
RefSeq	YP_001381067.1.
3D structure databases
SMR	A7HH87. Positions 3-492, 517-727.
ModBase	Search...
Protein-protein interaction databases
STRING	A7HH87.
Genome annotation databases
GeneID	5377443.
GenomeReviews	Gene locus Anae109_3904 in contig CP000769_GR.
KEGG	afw:Anae109_3904.
NMPDR	fig|404589.4.peg.3351.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0013.
HOGENOM	HBG354397.
OMA	GHTIEPS.
Family and domain databases
HAMAP	MF_00036_B. Ala_tRNA_synth_B. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018162. Ala-tRNA-synth_IIc_anticod-bd. IPR018165. Ala-tRNA-synth_IIc_core. IPR018164. Ala-tRNA-synth_IIc_N. IPR003156. Pesterase_DHHA1. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_ANADF

Accession

Primary (citable) accession number: A7HH87

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	September 11, 2007
Last modified:	February 9, 2010
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)