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A7HH70 (DDL_ANADF) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:Anae109_3887
OrganismAnaeromyxobacter sp. (strain Fw109-5) [Complete proteome] [HAMAP]
Taxonomic identifier404589 [NCBI]
Taxonomic lineageBacteriaProteobacteriaDeltaproteobacteriaMyxococcalesCystobacterineaeMyxococcaceaeAnaeromyxobacter

Protein attributes

Sequence length308 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 308308D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000341051

Regions

Domain105 – 302198ATP-grasp
Nucleotide binding133 – 18856ATP By similarity

Sites

Metal binding2561Magnesium or manganese 1 By similarity
Metal binding2691Magnesium or manganese 1 By similarity
Metal binding2691Magnesium or manganese 2 By similarity
Metal binding2711Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
A7HH70 [UniParc].

Last modified September 11, 2007. Version 1.
Checksum: 1D2BF3F39C9B980F

FASTA30832,500
        10         20         30         40         50         60 
MGKWTGKKVA VLYGGRSSER EVSLRTGAAC ADALRARGHD VTLVDVDAEL PARLRELRVD 

        70         80         90        100        110        120 
VAFVALHGRW GEDGCVQGLL ECMGIPYTGS GVTASAMGMD KTVSKAIFKA LGLDVIEYRA 

       130        140        150        160        170        180 
FPPARAAEID LRDLPFGVPC VVKPAGEGSS VGVQIVKDAA RLADACREAA RYKGDVVVER 

       190        200        210        220        230        240 
YVKGTEVNVA VLEGKALGAI EIAPANEFYD YAAKYTAGTT RYYYPARIPE AHASRVMAAA 

       250        260        270        280        290        300 
EAAHRGLGCS GVTRVDFIVA PDGTPFILEV NTLPGMTATS LVPKIAAGLG LAFPELCERI 


LDGAALKA 

« Hide

References

[1]"Complete sequence of Anaeromyxobacter sp. Fw109-5."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. expand/collapse author list , Lykidis A., Fields M., Richardson P.
Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Fw109-5.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000769 Genomic DNA. Translation: ABS28066.1.
RefSeqYP_001381050.1. NC_009675.1.

3D structure databases

ProteinModelPortalA7HH70.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING404589.Anae109_3887.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABS28066; ABS28066; Anae109_3887.
GeneID5377312.
KEGGafw:Anae109_3887.
PATRIC20933265. VBIAnaSp113478_4072.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMADTQYRIP.
OrthoDBEOG6ND0KB.
ProtClustDBPRK01372.

Enzyme and pathway databases

BioCycASP404589:GHMT-3937-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_ANADF
AccessionPrimary (citable) accession number: A7HH70
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: September 11, 2007
Last modified: February 19, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways