A7HH70 (DDL_ANADF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
D-alanine--D-alanine ligase
EC=6.3.2.4

Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase

Gene names

Name:	ddl
Ordered Locus Names:	Anae109_3887

Organism

Anaeromyxobacter sp. (strain Fw109-5) [Complete proteome] [HAMAP]

Taxonomic identifier

404589 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Anaeromyxobacter

Protein attributes

Sequence length	308 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the D-alanine--D-alanine ligase family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cell wall Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP D-alanine-D-alanine ligase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 308	308	D-alanine--D-alanine ligase HAMAP-Rule MF_00047		PRO_0000341051
Regions
	Domain	105 – 302	198	ATP-grasp
	Nucleotide binding	133 – 188	56	ATP By similarity
Sites
	Metal binding	256	1	Magnesium or manganese 1 By similarity
	Metal binding	269	1	Magnesium or manganese 1 By similarity
	Metal binding	269	1	Magnesium or manganese 2 By similarity
	Metal binding	271	1	Magnesium or manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7HH70 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: 1D2BF3F39C9B980F	FASTA	308	32,500
	10 20 30 40 50 60 MGKWTGKKVA VLYGGRSSER EVSLRTGAAC ADALRARGHD VTLVDVDAEL PARLRELRVD 70 80 90 100 110 120 VAFVALHGRW GEDGCVQGLL ECMGIPYTGS GVTASAMGMD KTVSKAIFKA LGLDVIEYRA 130 140 150 160 170 180 FPPARAAEID LRDLPFGVPC VVKPAGEGSS VGVQIVKDAA RLADACREAA RYKGDVVVER 190 200 210 220 230 240 YVKGTEVNVA VLEGKALGAI EIAPANEFYD YAAKYTAGTT RYYYPARIPE AHASRVMAAA 250 260 270 280 290 300 EAAHRGLGCS GVTRVDFIVA PDGTPFILEV NTLPGMTATS LVPKIAAGLG LAFPELCERI LDGAALKA

References

[1]

"Complete sequence of Anaeromyxobacter sp. Fw109-5." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Lykidis A., Fields M., Richardson P. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Fw109-5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000769 Genomic DNA. Translation: ABS28066.1.
RefSeq	YP_001381050.1. NC_009675.1.
3D structure databases
ProteinModelPortal	A7HH70.
ModBase	Search...
Protein-protein interaction databases
STRING	404589.Anae109_3887.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABS28066; ABS28066; Anae109_3887.
GeneID	5377312.
KEGG	afw:Anae109_3887.
PATRIC	20933265. VBIAnaSp113478_4072.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1181.
HOGENOM	HOG000011592.
KO	K01921.
OMA	VEDASCD.
ProtClustDB	PRK01372.
Enzyme and pathway databases
BioCyc	ASP404589:GHMT-3941-MONOMER.
UniPathway	UPA00219.
Family and domain databases
Gene3D	3.30.1490.20. 1 hit. 3.30.470.20. 2 hits. 3.40.50.20. 1 hit.
HAMAP	MF_00047. Dala_Dala_lig.
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR000291. D-Ala_lig_Van_CS. IPR005905. D_ala_D_ala. IPR011095. Dala_Dala_lig_C. IPR011127. Dala_Dala_lig_N. IPR016185. PreATP-grasp_dom. [Graphical view]
PANTHER	PTHR23132. PTHR23132. 1 hit.
Pfam	PF07478. Dala_Dala_lig_C. 1 hit. PF01820. Dala_Dala_lig_N. 2 hits. [Graphical view]
SUPFAM	SSF52440. PreATP-grasp-like. 1 hit.
TIGRFAMs	TIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00843. DALA_DALA_LIGASE_1. 1 hit. PS00844. DALA_DALA_LIGASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DDL_ANADF

Accession

Primary (citable) accession number: A7HH70

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	September 11, 2007
Last modified:	May 1, 2013
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program