A7HH69 (MURB_ANADF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylenolpyruvoylglucosamine reductase
EC=1.1.1.158

Alternative name(s):
UDP-N-acetylmuramate dehydrogenase

Gene names

Name:	murB
Ordered Locus Names:	Anae109_3886

Organism

Anaeromyxobacter sp. (strain Fw109-5) [Complete proteome] [HAMAP]

Taxonomic identifier

404589 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Anaeromyxobacter

Protein attributes

Sequence length	318 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation By similarity. HAMAP MF_00037
Catalytic activity	UDP-N-acetylmuramate + NADP+ = UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH. HAMAP MF_00037
Cofactor	FAD By similarity. HAMAP MF_00037
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00037
Subcellular location	Cytoplasm By similarity HAMAP MF_00037.
Sequence similarities	Belongs to the MurB family. Contains 1 FAD-binding PCMH-type domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	UDP-N-acetylmuramate dehydrogenase activity Inferred from electronic annotation. Source: EC flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 318	318	UDP-N-acetylenolpyruvoylglucosamine reductase HAMAP MF_00037		PRO_0000332446
Regions
	Domain	39 – 202	164	FAD-binding PCMH-type
Sites
	Active site	182	1	By similarity
	Active site	231	1	Proton donor By similarity
	Active site	301	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7HH69 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: 5853B7AAC97DBF5D	FASTA	318	33,233
	10 20 30 40 50 60 MAGALRGPGD ADWRAEIERR VRGEVLRDAP LAPRTAVRVG GPADLLVRPA DPGALAALLR 70 80 90 100 110 120 AVRELSVPLT ILGGGANTLV ADAGVRGVVL RLPQGFGEEA RDGERLVLGA GAPTSRLWVR 130 140 150 160 170 180 AHAAGLVGIE FVAGIPGTLG GAVAMNAGTK IGEMKDVVSA VELATADGAG FVPAASLGFA 190 200 210 220 230 240 YRTCRLPAGA VITRVQLTLR PGDVAESERI MQADRDGRRR TQPLDRPTFG STFTNPPGDF 250 260 270 280 290 300 AGRLVEAVGL KGHRVGGATW SDVHANFVSN LGGATARDVL ALMRLARTRV KQRFGISLET 310 EVRLVGEFHA EDLEGLRA

References

[1]

"Complete sequence of Anaeromyxobacter sp. Fw109-5." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Lykidis A., Fields M., Richardson P. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Fw109-5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000769 Genomic DNA. Translation: ABS28065.1.
RefSeq	YP_001381049.1. NC_009675.1.
3D structure databases
ProteinModelPortal	A7HH69.
ModBase	Search...
Protein-protein interaction databases
STRING	A7HH69.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5377311.
GenomeReviews	Gene locus Anae109_3886 in contig CP000769_GR.
KEGG	afw:Anae109_3886.
NMPDR	fig|404589.4.peg.3338.
PATRIC	20933263. VBIAnaSp113478_4071.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0812.
HOGENOM	HBG686573.
OMA	EANVGRV.
ProtClustDB	PRK14652.
Family and domain databases
HAMAP	MF_00037. MurB. [Tree]
InterPro	IPR016169. CO_DH_flavot_FAD-bd_sub2. IPR016166. FAD-bd_2. IPR016167. FAD-bd_2_sub1. IPR003170. MurB. IPR011601. MurB_C. IPR006094. Oxid_FAD_bind_N. [Graphical view]
Gene3D	G3DSA:3.30.465.10. CO_DH_flavoprot_FAD-bd_sub2. 1 hit. G3DSA:3.30.43.10. FAD-binding_2_sub1. 1 hit. G3DSA:3.90.78.10. MurB_C. 1 hit.
KO	K00075.
PANTHER	PTHR21071. MurB. 1 hit.
Pfam	PF01565. FAD_binding_4. 1 hit. PF02873. MurB_C. 1 hit. [Graphical view]
SUPFAM	SSF56176. FAD-binding_2. 1 hit. SSF56194. MurB_C. 1 hit.
TIGRFAMs	TIGR00179. MurB. 1 hit.
PROSITE	PS51387. FAD_PCMH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MURB_ANADF

Accession

Primary (citable) accession number: A7HH69

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	September 11, 2007
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program