Reviewed, UniProtKB/Swiss-Prot A7HH68 (MURC_ANADF)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramate--L-alanine ligase
    EC=6.3.2.8
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanine synthetase

Gene names

Name:	murC
Ordered Locus Names:	Anae109_3885

Organism

Anaeromyxobacter sp. (strain Fw109-5) [Complete proteome] [HAMAP]

Taxonomic identifier

404589 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Anaeromyxobacter

Protein attributes

Sequence length	458 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity. HAMAP MF_00046
Catalytic activity	ATP + UDP-N-acetylmuramate + L-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine. HAMAP MF_00046
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00046
Subcellular location	Cytoplasm Probable HAMAP MF_00046.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramate-L-alanine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 458	458	UDP-N-acetylmuramate--L-alanine ligase HAMAP MF_00046		PRO_1000004307
Regions
	Nucleotide binding	115 – 121	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	A7HH68-1 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: 2652A4E71E61D811	FASTA	458	49,500
	10 20 30 40 50 60 MSLFRSRPAK IHFVGIGGIG MSGIAEVLLN LGYAVSGSDL KESEITRRLA SLGGRVQRGH 70 80 90 100 110 120 AAQHVEQVDV VVTSSAVRKD NPEVVEARRR KIPIIPRAEM LAELMRLKYG VAIAGSHGKT 130 140 150 160 170 180 TTTSLAAHLL AHAGLDPTAV VGGKVNTFGS NAKLGRGDYM VVEADESDGS FLHIPPTIAI 190 200 210 220 230 240 VTNIDPEHLD HWKTEEALRK GFLDFVNRVP FYGRAILCID HPTVQSLLPE VESRYVTYGE 250 260 270 280 290 300 SHQADYRADG IEVSGHAVRF DAFRRDEPLG RFEVRLVGRH NALNALAVVA IADEMGVPAD 310 320 330 340 350 360 VTREALASFG GVQRRFTVRG EAGGVTVVDD YGHHPAEVKA TLLGAREAFH RRVVCLFQPH 370 380 390 400 410 420 RYTRTRDLLP EFATAFNDAD VLLLTDIYAA GEEPIPGATS EALAEAIRAC GHRDVSLVPR 430 440 450 PELARAARQR VRPGDIVLTL GAGDITAAGP ELLGLLEA

References

[1]

"Complete sequence of Anaeromyxobacter sp. Fw109-5." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Lykidis A., Fields M., Richardson P. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000769 Genomic DNA. Translation: ABS28064.1.
RefSeq	YP_001381048.1.
3D structure databases
SMR	A7HH68. Positions 7-456.
ModBase	Search...
Protein-protein interaction databases
STRING	A7HH68.
Genome annotation databases
GeneID	5377310.
GenomeReviews	Gene locus Anae109_3885 in contig CP000769_GR.
KEGG	afw:Anae109_3885.
NMPDR	fig|404589.4.peg.3337.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0773.
HOGENOM	HBG476594.
OMA	YQPHRYS.
Family and domain databases
HAMAP	MF_00046. MurC. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR016040. NAD(P)-bd_dom. IPR005758. UDP-N-AcMur_Ala_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01082. murC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURC_ANADF

Accession

Primary (citable) accession number: A7HH68

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 11, 2007
Last modified:	February 9, 2010
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)