Reviewed, UniProtKB/Swiss-Prot A7HH64 (MRAY_ANADF)

Names and origin

Protein names

Recommended name:
    Phospho-N-acetylmuramoyl-pentapeptide-transferase
    EC=2.7.8.13
Alternative name(s):
    UDP-MurNAc-pentapeptide phosphotransferase

Gene names

Name:	mraY
Ordered Locus Names:	Anae109_3881

Organism

Anaeromyxobacter sp. (strain Fw109-5) [Complete proteome] [HAMAP]

Taxonomic identifier

404589 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Anaeromyxobacter

Protein attributes

Sequence length	380 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	First step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan By similarity.
Catalytic activity	UDP-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate = UMP + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol. HAMAP MF_00038
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00038
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase 4 family. MraY subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	phospho-N-acetylmuramoyl-pentapeptide-transferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 380	380	Phospho-N-acetylmuramoyl-pentapeptide-transferase HAMAP MF_00038		PRO_1000002933
Regions
	Transmembrane	26 – 46	21	Potential
	Transmembrane	75 – 95	21	Potential
	Transmembrane	98 – 118	21	Potential
	Transmembrane	135 – 155	21	Potential
	Transmembrane	160 – 180	21	Potential
	Transmembrane	183 – 203	21	Potential
	Transmembrane	222 – 242	21	Potential
	Transmembrane	259 – 279	21	Potential
	Transmembrane	283 – 303	21	Potential
	Transmembrane	311 – 331	21	Potential
	Transmembrane	357 – 377	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	A7HH64-1 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: 342F30B83C5D0981	FASTA	380	41,602
	10 20 30 40 50 60 MLYHFLYPLS GQFGLFNVLR YPSFRIVAAG LVSLLIGLLL GPLFIERMRV LQYGHSNVRE 70 80 90 100 110 120 DTPERHKKKA GTPSMGGALI LLAVTVSTLL FADLGNRLVW AALLVTLGYG VIGFWDDWLK 130 140 150 160 170 180 ISKRNSKGLA GKKKLVLQVL VVLAVYYGLL TDWQPRTTDG FPFLTIGSLV DLHLTLPFVP 190 200 210 220 230 240 THLFSPSLGW LYLPFMIFVV VATSNAVNLT DGLDGLAIGP TIVSSMTFLA LSYVAGATIA 250 260 270 280 290 300 GFSLAEYLRI AYIPGAEELG VFCSAIFGAG IAFLWYNTYP ASVFMGDVGS LALGGGLGML 310 320 330 340 350 360 AVLTKNEVAS AILHGVFLAE TVSVILQVWS FRTTGKRIFR MAPIHHHYEL KGWAEPKIIV 370 380 RFWIMSIMLA LVALMSLKLR

References

[1]

"Complete sequence of Anaeromyxobacter sp. Fw109-5." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Lykidis A., Fields M., Richardson P. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000769 Genomic DNA. Translation: ABS28060.1.
RefSeq	YP_001381044.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A7HH64.
Genome annotation databases
GeneID	5375807.
GenomeReviews	Gene locus Anae109_3881 in contig CP000769_GR.
KEGG	afw:Anae109_3881.
NMPDR	fig|404589.4.peg.3333.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	HHHFEQK.
Family and domain databases
HAMAP	MF_00038. [Tree]
InterPro	IPR000715. Glycosyl_transferase_4. IPR018481. Glycosyl_Trfase_4_cons-reg. IPR003524. PNAcMuramoyl-5peptid_Trfase. IPR018480. PNAcMuramoyl-5peptid_Trfase_CS. [Graphical view]
PANTHER	PTHR22926. Glyco_trans_4. 1 hit. PTHR22926:SF3. PNAcPpept_trans. 1 hit.
Pfam	PF00953. Glycos_transf_4. 1 hit. PF10555. MraY_sig1. 1 hit. [Graphical view]
TIGRFAMs	TIGR00445. mraY. 1 hit.
PROSITE	PS01347. MRAY_1. 1 hit. PS01348. MRAY_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MRAY_ANADF

Accession

Primary (citable) accession number: A7HH64

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 11, 2007
Last modified:	November 3, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)