Reviewed, UniProtKB/Swiss-Prot A7HGZ2 (PANB_ANADF)

Names and origin

Protein names

Recommended name:
    3-methyl-2-oxobutanoate hydroxymethyltransferase
    EC=2.1.2.11
Alternative name(s):
    Ketopantoate hydroxymethyltransferase
      Short name=KPHMT

Gene names

Name:	panB
Ordered Locus Names:	Anae109_3809

Organism

Anaeromyxobacter sp. (strain Fw109-5) [Complete proteome] [HAMAP]

Taxonomic identifier

404589 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Anaeromyxobacter

Protein attributes

Sequence length	305 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate By similarity.
Catalytic activity	5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = tetrahydrofolate + 2-dehydropantoate. HAMAP MF_00156
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantoate from 3-methyl-2-oxobutanoate: step 1/2. HAMAP MF_00156
Subunit structure	Homodecamer; pentamer of dimers By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the panB family.

Ontologies

Keywords
Biological process	Pantothenate biosynthesis
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pantothenate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-methyl-2-oxobutanoate hydroxymethyltransferase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 305	305	3-methyl-2-oxobutanoate hydroxymethyltransferase HAMAP MF_00156		PRO_1000011360
Regions
	Region	52 – 53	2	Alpha-ketoisovalerate binding By similarity
Sites
	Active site	194	1	Proton acceptor By similarity
	Metal binding	52	1	Magnesium By similarity
	Metal binding	95	1	Magnesium By similarity
	Metal binding	127	1	Magnesium By similarity
	Binding site	95	1	Alpha-ketoisovalerate By similarity
	Binding site	125	1	Alpha-ketoisovalerate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7HGZ2-1 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: 25B9DE6E3EAE64A7	FASTA	305	32,223
	10 20 30 40 50 60 MSSQTAPRRR VTIHELRRMK DAGEKIAVVT AYDATAARLA DAAGVDVVLV GDSLGMVVQG 70 80 90 100 110 120 HESTLPVTLE HMIYHSAAVR RGLARSSGRA HLVGDMPFGS YQASADEAVK SAMRLVAEGG 130 140 150 160 170 180 VESVKLEGGA EFVEVIRRIA RAGVPVMGHI GLTPQAVHRM GGYVVQGKDS EKAQQLLRDA 190 200 210 220 230 240 RALELAGCYA IVLECIPAEL ARIISSQLRI PTIGIGAGLH CDGQVLVLND LLGMDDAFTP 250 260 270 280 290 300 KFVKRFGEIG QAISTAVGAY VGEVKRRVFP DDAHSFHSST VRLVPAAPVD EDDGEPSGGV MGAPV

References

[1]

"Complete sequence of Anaeromyxobacter sp. Fw109-5." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Lykidis A., Fields M., Richardson P. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000769 Genomic DNA. Translation: ABS27988.1.
RefSeq	YP_001380972.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A7HGZ2.
Genome annotation databases
GeneID	5375233.
GenomeReviews	Gene locus Anae109_3809 in contig CP000769_GR.
KEGG	afw:Anae109_3809.
NMPDR	fig|404589.4.peg.3270.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	YATPEQT.
Family and domain databases
HAMAP	MF_00156. [Tree]
InterPro	IPR003700. Pantoate_hydroxy_MeTrfase. IPR015813. Pyrv/PenolPyrv_Kinase_cat. [Graphical view]
Gene3D	G3DSA:3.20.20.60. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
PANTHER	PTHR20881. Pantoate_transf. 1 hit.
Pfam	PF02548. Pantoate_transf. 1 hit. [Graphical view]
PIRSF	PIRSF000388. Pantoate_hydroxy_MeTrfase. 1 hit.
TIGRFAMs	TIGR00222. panB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PANB_ANADF

Accession

Primary (citable) accession number: A7HGZ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 11, 2007
Last modified:	November 3, 2009
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)