Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

A7HG97

- BIOB_ANADF

UniProt

A7HG97 - BIOB_ANADF

Protein

Biotin synthase

Gene

bioB

Organism
Anaeromyxobacter sp. (strain Fw109-5)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 49 (01 Oct 2014)
      Sequence version 1 (11 Sep 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

    Catalytic activityi

    Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
    Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi64 – 641Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi68 – 681Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi71 – 711Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi109 – 1091Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi142 – 1421Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi202 – 2021Iron-sulfur 2 (2Fe-2S)UniRule annotation

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. biotin synthase activity Source: UniProtKB-HAMAP
    4. iron ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Biotin biosynthesis

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciASP404589:GHMT-3607-MONOMER.
    UniPathwayiUPA00078; UER00162.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
    Gene namesi
    Name:bioBUniRule annotation
    Ordered Locus Names:Anae109_3562
    OrganismiAnaeromyxobacter sp. (strain Fw109-5)
    Taxonomic identifieri404589 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaMyxococcalesCystobacterineaeAnaeromyxobacteraceaeAnaeromyxobacter
    ProteomesiUP000006382: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 368368Biotin synthasePRO_0000381197Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi404589.Anae109_3562.

    Structurei

    3D structure databases

    ProteinModelPortaliA7HG97.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0502.
    HOGENOMiHOG000239958.
    KOiK01012.
    OMAiCKEDCIF.
    OrthoDBiEOG622PMP.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01694. BioB.
    InterProiIPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001619. Biotin_synth. 1 hit.
    SMARTiSM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00433. bioB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A7HG97-1 [UniParc]FASTAAdd to Basket

    « Hide

    MCETARTLPP DIVPITVEEA RTLIRHTGGP ALDALLDRAD AVRRAVHGDE    50
    VALCGITNAK SGRCPENCGF CSQSAHFPGA DAPVYPLVPA EEMVAQAKVA 100
    ERAGAREFSI VTSGTRVAKE SELATIEEAV RKLRAETAVE PCASLGLVRE 150
    PELVRLKAAG LMHYHHNLET ARSFFENVCT THTYDEQLET IRAAKRQGLK 200
    LCSGGILGMG ETPEQRVELA ATIRELGIDC VPMNFLNPRP GTPMEHVQAI 250
    TAEECLAAVA VFRLMMPAAH IFVMGGREVN LGGRQHLIFR AGANGTMVGN 300
    YLTSAGRGPG ETVRMVEEQG LRLRAPDTGR EWAFDGSAPA EAEWNRRAAE 350
    PGGKRGLPVV GPPRGGCA 368
    Length:368
    Mass (Da):39,458
    Last modified:September 11, 2007 - v1
    Checksum:i0EFB16665DD082E2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000769 Genomic DNA. Translation: ABS27743.1.
    RefSeqiWP_012098367.1. NC_009675.1.
    YP_001380727.1. NC_009675.1.

    Genome annotation databases

    EnsemblBacteriaiABS27743; ABS27743; Anae109_3562.
    GeneIDi5378141.
    KEGGiafw:Anae109_3562.
    PATRICi20932571. VBIAnaSp113478_3728.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000769 Genomic DNA. Translation: ABS27743.1 .
    RefSeqi WP_012098367.1. NC_009675.1.
    YP_001380727.1. NC_009675.1.

    3D structure databases

    ProteinModelPortali A7HG97.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 404589.Anae109_3562.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABS27743 ; ABS27743 ; Anae109_3562 .
    GeneIDi 5378141.
    KEGGi afw:Anae109_3562.
    PATRICi 20932571. VBIAnaSp113478_3728.

    Phylogenomic databases

    eggNOGi COG0502.
    HOGENOMi HOG000239958.
    KOi K01012.
    OMAi CKEDCIF.
    OrthoDBi EOG622PMP.

    Enzyme and pathway databases

    UniPathwayi UPA00078 ; UER00162 .
    BioCyci ASP404589:GHMT-3607-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01694. BioB.
    InterProi IPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001619. Biotin_synth. 1 hit.
    SMARTi SM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00433. bioB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Fw109-5.

    Entry informationi

    Entry nameiBIOB_ANADF
    AccessioniPrimary (citable) accession number: A7HG97
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: September 11, 2007
    Last modified: October 1, 2014
    This is version 49 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3