A7HDA1 (SYC_ANADF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cysteine--tRNA ligase
EC=6.1.1.16

Alternative name(s):
Cysteinyl-tRNA synthetase
Short name=CysRS

Gene names

Name:	cysS
Ordered Locus Names:	Anae109_2496

Organism

Anaeromyxobacter sp. (strain Fw109-5) [Complete proteome] [HAMAP]

Taxonomic identifier

404589 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Anaeromyxobacter

Protein attributes

Sequence length	482 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP MF_00041
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00041
Subunit structure	Monomer By similarity. HAMAP MF_00041
Subcellular location	Cytoplasm HAMAP MF_00041.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cysteine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 482	482	Cysteine--tRNA ligase HAMAP MF_00041		PRO_1000071068
Regions
	Motif	31 – 41	11	"HIGH" region HAMAP MF_00041
	Motif	272 – 276	5	"KMSKS" region HAMAP MF_00041
Sites
	Metal binding	29	1	Zinc By similarity
	Metal binding	210	1	Zinc By similarity
	Metal binding	235	1	Zinc By similarity
	Metal binding	239	1	Zinc By similarity
	Binding site	275	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7HDA1 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: BBFF44420DF09F73	FASTA	482	53,232
	10 20 30 40 50 60 MPIVISDTLT GTKRELQPIE PGKIRFYACG PTVYDSAHVG HGRSYVVWDL VVRHLRARGY 70 80 90 100 110 120 EVRYVRNFTD VDDKIIRRAT ERREDPVALA ARYAREFDED MDALGNLRPD VAPKVSEHVP 130 140 150 160 170 180 QIVAMISSLV EKGFAYAAAN GDVYFSVRAF PDYGRLSKRN LDDLLSGARV EPGEAKRDPL 190 200 210 220 230 240 DFALWKAAKP GEPTWDSPWG GGRPGWHIEC SAMTLAHLGV PIDLHGGGKD LVFPHHTNEI 250 260 270 280 290 300 AQSAAAVGDG RTAESFCRHW MHHGFVEIDS EKMSKSLGNF FTLRDVLAKF DAEGVRLFYL 310 320 330 340 350 360 GTHYRNPINY SDAILAEAER RLNYFYETLE KVDALAEGTS PAGEGGGVVE DARRALDDDF 370 380 390 400 410 420 NAPQVLAVLA EAFTAANALA DKRGKKTPED RARLAAFARD VREIGTTLGL VQRRPAEALR 430 440 450 460 470 480 AIRARAAARR GIDPASVEAK IVERAEARRA RDFARSDAIR DALLAQGVAL MDGPQGTTWK VE

References

[1]

"Complete sequence of Anaeromyxobacter sp. Fw109-5." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Lykidis A., Fields M., Richardson P. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Fw109-5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000769 Genomic DNA. Translation: ABS26697.1.
RefSeq	YP_001379681.1. NC_009675.1.
3D structure databases
ProteinModelPortal	A7HDA1.
ModBase	Search...
Protein-protein interaction databases
STRING	A7HDA1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5377253.
GenomeReviews	Gene locus Anae109_2496 in contig CP000769_GR.
KEGG	afw:Anae109_2496.
PATRIC	20930299. VBIAnaSp113478_2614.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0215.
HOGENOM	HBG327651.
OMA	DFDALNM.
ProtClustDB	PRK00260.
Family and domain databases
HAMAP	MF_00041. Cys_tRNA_synth. [Tree]
InterPro	IPR015803. Cys-tRNA-synt. IPR015273. Cys-tRNA-synt_Ia_DALR. IPR024909. Cys-tRNA/MSH_ligase. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01883.
PANTHER	PTHR10890. Cys_tRNA-synt_1a. 1 hit.
Pfam	PF09190. DALR_2. 1 hit. PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
SMART	SM00840. DALR_2. 1 hit. [Graphical view]
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00435. CysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYC_ANADF

Accession

Primary (citable) accession number: A7HDA1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	September 11, 2007
Last modified:	January 25, 2012
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program