Reviewed, UniProtKB/Swiss-Prot A7HD76 (PYRD_ANADF)

Names and origin

Protein names

Recommended name:
    Dihydroorotate dehydrogenase
    EC=1.3.5.2
Alternative name(s):
    Dihydroorotate oxidase
    DHOdehase
      Short name=DHODase
      Short name=DHOD

Gene names

Name:	pyrD
Ordered Locus Names:	Anae109_2471

Organism

Anaeromyxobacter sp. (strain Fw109-5) [Complete proteome] [HAMAP]

Taxonomic identifier

404589 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Anaeromyxobacter

Protein attributes

Sequence length	363 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + a quinone = orotate + a quinol. HAMAP MF_00225
Cofactor	Binds 1 FMN per subunit By similarity. HAMAP MF_00225
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1. HAMAP MF_00225
Subunit structure	Homodimer By similarity. HAMAP MF_00225
Subcellular location	Cell membrane; Peripheral membrane protein By similarity HAMAP MF_00225.
Sequence similarities	Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Cellular component	Cell membrane Membrane
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro UMP biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	dihydroorotate oxidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 363	363	Dihydroorotate dehydrogenase HAMAP MF_00225		PRO_1000024150
Sites
	Active site	178	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7HD76-1 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: 0C2617661F255A9B	FASTA	363	38,382
	10 20 30 40 50 60 MIWPALRWTL FHLDPERAHR LAHGALHRVP PGLARLRRPA VPPELRVSCL GLDFDGPIGL 70 80 90 100 110 120 AAGFDKGDAS IAGLFALGFS HVEIGTITPR PQAGNEPPRL FRLVEHRALV NRMGFNNAGA 130 140 150 160 170 180 EVCARRLAGV PATARMGPVG VNVGKNKTTP NEDAAADYLA CIDRLHPYAD YLVVNISSPN 190 200 210 220 230 240 TPGLRQLQER DQLDALLRAC AGRLRERAPG KPLLVKLAPD LSPTALDEAV DVAIDAGVSG 250 260 270 280 290 300 IVATNTTLSR AGVERHPRAR EAGGLSGAPL EALATSVVRR CYIRAAGRVP IVGCGGVMNA 310 320 330 340 350 360 EGAYAKIRAG ATLVQVYTGL VYGGPGFVRR LNDGLARLLA RDGFRTVAEA VGADVETAER AGV

References

[1]

"Complete sequence of Anaeromyxobacter sp. Fw109-5." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Lykidis A., Fields M., Richardson P. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000769 Genomic DNA. Translation: ABS26672.1.
RefSeq	YP_001379656.1.
3D structure databases
SMR	A7HD76. Positions 10-356.
ModBase	Search...
Protein-protein interaction databases
STRING	A7HD76.
Genome annotation databases
GeneID	5377763.
GenomeReviews	Gene locus Anae109_2471 in contig CP000769_GR.
KEGG	afw:Anae109_2471.
NMPDR	fig|404589.4.peg.2140.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0167.
HOGENOM	HBG351027.
OMA	AALNRMG.
Family and domain databases
HAMAP	MF_00225. DHO_dh_type2. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR012135. Dihydroorotate_DH_1_2. IPR005719. Dihydroorotate_DH_2. IPR001295. Dihydroorotate_DH_CS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF01180. DHO_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000164. DHO_oxidase. 1 hit.
TIGRFAMs	TIGR01036. pyrD_sub2. 1 hit.
PROSITE	PS00911. DHODEHASE_1. 1 hit. PS00912. DHODEHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRD_ANADF

Accession

Primary (citable) accession number: A7HD76

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 11, 2007
Last modified:	February 9, 2010
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)