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A7HD76 (PYRD_ANADF) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 35. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Dihydroorotate dehydrogenase (quinone)
EC=1.3.5.2
Alternative name(s):
DHOdehase
Short name=DHOD
Short name=DHODase
Dihydroorotate oxidase
Gene names
Name:pyrD
Ordered Locus Names:Anae109_2471
OrganismAnaeromyxobacter sp. (strain Fw109-5) [Complete proteome] [HAMAP]
Taxonomic identifier404589 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Anaeromyxobacter

Protein attributes

Sequence length363 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor By similarity. HAMAP MF_00225

Catalytic activity

(S)-dihydroorotate + a quinone = orotate + a quinol. HAMAP MF_00225

Cofactor

Binds 1 FMN per subunit By similarity. HAMAP MF_00225

Pathway

Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1. HAMAP MF_00225

Subunit structure

Monomer By similarity. HAMAP MF_00225

Subcellular location

Cell membrane; Peripheral membrane protein By similarity HAMAP MF_00225.

Sequence similarities

Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.

Ontologies

Keywords
   Biological processPyrimidine biosynthesis
   Cellular componentCell membrane
Membrane
   LigandFMN
Flavoprotein
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological process'de novo' pyrimidine base biosynthetic process

Inferred from electronic annotation. Source: InterPro

UMP biosynthetic process

Inferred from electronic annotation. Source: InterPro

   Cellular componentplasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functiondihydroorotate oxidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 363363Dihydroorotate dehydrogenase (quinone) HAMAP MF_00225
PRO_1000024150

Regions

Nucleotide binding62 – 665FMN By similarity
Nucleotide binding317 – 3182FMN By similarity
Region111 – 1155Substrate binding By similarity
Region245 – 2462Substrate binding By similarity

Sites

Active site1781Nucleophile By similarity
Binding site661Substrate By similarity
Binding site861FMN; via amide nitrogen By similarity
Binding site1421FMN By similarity
Binding site1751FMN By similarity
Binding site1751Substrate By similarity
Binding site1801Substrate By similarity
Binding site2161FMN By similarity
Binding site2441FMN; via carbonyl oxygen By similarity
Binding site2671FMN; via amide nitrogen By similarity
Binding site2961FMN; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
A7HD76 [UniParc].

Last modified September 11, 2007. Version 1.
Checksum: 0C2617661F255A9B

FASTA36338,382
        10         20         30         40         50         60 
MIWPALRWTL FHLDPERAHR LAHGALHRVP PGLARLRRPA VPPELRVSCL GLDFDGPIGL 

        70         80         90        100        110        120 
AAGFDKGDAS IAGLFALGFS HVEIGTITPR PQAGNEPPRL FRLVEHRALV NRMGFNNAGA 

       130        140        150        160        170        180 
EVCARRLAGV PATARMGPVG VNVGKNKTTP NEDAAADYLA CIDRLHPYAD YLVVNISSPN 

       190        200        210        220        230        240 
TPGLRQLQER DQLDALLRAC AGRLRERAPG KPLLVKLAPD LSPTALDEAV DVAIDAGVSG 

       250        260        270        280        290        300 
IVATNTTLSR AGVERHPRAR EAGGLSGAPL EALATSVVRR CYIRAAGRVP IVGCGGVMNA 

       310        320        330        340        350        360 
EGAYAKIRAG ATLVQVYTGL VYGGPGFVRR LNDGLARLLA RDGFRTVAEA VGADVETAER 


AGV

« Hide

References

[1]"Complete sequence of Anaeromyxobacter sp. Fw109-5."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. expand/collapse author list , Lykidis A., Fields M., Richardson P.
Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Fw109-5.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000769 Genomic DNA. Translation: ABS26672.1.
RefSeqYP_001379656.1. NC_009675.1.

3D structure databases

ProteinModelPortalA7HD76.
ModBaseSearch...

Protein-protein interaction databases

STRINGA7HD76.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID5377763.
GenomeReviewsGene locus Anae109_2471 in contig CP000769_GR.
KEGGafw:Anae109_2471.
NMPDRfig|404589.4.peg.2140.
PATRIC20930247. VBIAnaSp113478_2588.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0167.
HOGENOMHBG351027.
OMAAALNRMG.
ProtClustDBPRK05286.

Family and domain databases

HAMAPMF_00225. DHO_dh_type2.
[Tree]
InterProIPR013785. Aldolase_TIM.
IPR012135. Dihydroorotate_DH_1_2.
IPR005719. Dihydroorotate_DH_2.
IPR001295. Dihydroorotate_DH_CS.
[Graphical view]
Gene3DG3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KOK00226.
PfamPF01180. DHO_dh. 1 hit.
[Graphical view]
PIRSFPIRSF000164. DHO_oxidase. 1 hit.
TIGRFAMsTIGR01036. PyrD_sub2. 1 hit.
PROSITEPS00911. DHODEHASE_1. 1 hit.
PS00912. DHODEHASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePYRD_ANADF
AccessionPrimary (citable) accession number: A7HD76
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: September 11, 2007
Last modified: January 25, 2012
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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