Reviewed, UniProtKB/Swiss-Prot A7HCQ8 (ISPH_ANADF)

Names and origin

Protein names

Recommended name:
    4-hydroxy-3-methylbut-2-enyl diphosphate reductase
    EC=1.17.1.2

Gene names

Name:	ispH
Ordered Locus Names:	Anae109_2302

Organism

Anaeromyxobacter sp. (strain Fw109-5) [Complete proteome] [HAMAP]

Taxonomic identifier

404589 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Anaeromyxobacter

Protein attributes

Sequence length	283 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) By similarity.
Catalytic activity	Isopentenyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191 Dimethylallyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191
Cofactor	Binds 1 3Fe-4S cluster By similarity.
Pathway	Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1. HAMAP MF_00191 Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6. HAMAP MF_00191
Sequence similarities	Belongs to the ispH family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	3Fe-4S Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 283	283	4-hydroxy-3-methylbut-2-enyl diphosphate reductase HAMAP MF_00191		PRO_1000021083

Sequences

	Sequence		Length	Mass (Da)
	A7HCQ8-1 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: 199C6FF1A9374B2F	FASTA	283	30,142
	10 20 30 40 50 60 MEVKIAKTAG FCWGVRRTVD KVMEVADHAS GPVVTLGPII HNPQAVARFR EKGVGTVDAV 70 80 90 100 110 120 GEVGDGTTVV VRTHGAVKEE LDRAEGRGLE VVDGTCPYVK YPQAIAQRLS AEGYHVVIVG 130 140 150 160 170 180 DANHAEIKGV ISYSEGPCTV VKPGGPIPEI PAKKVAVIAQ TTCIGADFEA VVGALALRHK 190 200 210 220 230 240 EVRAVNTICN DTEERQADAR SLAREVDAVV VVGGKNSANT RHLAEICREI QPRTWHVETE 250 260 270 280 AELRPEWFTG CQVVGLSAGA STPDWVVEGV AGWLRGLHRP GTP

References

[1]

"Complete sequence of Anaeromyxobacter sp. Fw109-5." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Lykidis A., Fields M., Richardson P. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000769 Genomic DNA. Translation: ABS26504.1.
RefSeq	YP_001379488.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A7HCQ8.
Genome annotation databases
GeneID	5376104.
GenomeReviews	Gene locus Anae109_2302 in contig CP000769_GR.
KEGG	afw:Anae109_2302.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	REENIEF.
Family and domain databases
HAMAP	MF_00191. [Tree]
InterPro	IPR003451. LytB. [Graphical view]
Pfam	PF02401. LYTB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00216. ispH_lytB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPH_ANADF

Accession

Primary (citable) accession number: A7HCQ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 11, 2007
Last modified:	November 3, 2009
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)