Reviewed, UniProtKB/Swiss-Prot A7HBQ1 (TRMD_ANADF)

Names and origin

Protein names

Recommended name:
    tRNA (guanine-N(1)-)-methyltransferase
    EC=2.1.1.31
Alternative name(s):
    M1G-methyltransferase
    tRNA [GM37] methyltransferase

Gene names

Name:	trmD
Ordered Locus Names:	Anae109_1944

Organism

Anaeromyxobacter sp. (strain Fw109-5) [Complete proteome] [HAMAP]

Taxonomic identifier

404589 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Anaeromyxobacter

Protein attributes

Sequence length	248 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically methylates guanosine-37 in various tRNAs By similarity.
Catalytic activity	S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N(1)-methylguanine. HAMAP MF_00605
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the RNA methyltransferase trmD family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA modification Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine-N1-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 248	248	tRNA (guanine-N(1)-)-methyltransferase HAMAP MF_00605		PRO_1000006446
Regions
	Region	135 – 140	6	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	116	1	S-adenosyl-L-methionine; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7HBQ1-1 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: F3ECA092A487B240	FASTA	248	27,047
	10 20 30 40 50 60 MSARLEVEIL TLFPRMCEGY LAESILGKAR EAGIVSVSVS DVREHARGKH RVADDAPYGG 70 80 90 100 110 120 GAGMVMKPEP LTEAIEAARA RLPGALVALT SPRGARLDQA LARRLATHGR LVLVCGRYEG 130 140 150 160 170 180 VDERVLAAVD MEVSIGDFVL TGGELAALCV VDAAARLVPG VLGNEASAGA ESFAGEDTLL 190 200 210 220 230 240 EYPQYTRPPD FRGMKVPEVL LSGDHRRIER WRRREALRVT RERRPDLLER ARLTENDLRL IDAGDDEL

References

[1]

"Complete sequence of Anaeromyxobacter sp. Fw109-5." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Lykidis A., Fields M., Richardson P. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000769 Genomic DNA. Translation: ABS26147.1.
RefSeq	YP_001379131.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A7HBQ1.
Genome annotation databases
GeneID	5377425.
GenomeReviews	Gene locus Anae109_1944 in contig CP000769_GR.
KEGG	afw:Anae109_1944.
NMPDR	fig|404589.4.peg.1679.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	HRSVDDT.
Family and domain databases
HAMAP	MF_00605. [Tree]
InterPro	IPR016009. tRNA_m1G_MeTrfase. IPR002649. tRNA_m1G_MeTrfase_bac. [Graphical view]
Pfam	PF01746. tRNA_m1G_MT. 1 hit. [Graphical view]
PIRSF	PIRSF000386. tRNA_mtase. 1 hit.
ProDom	PD004978. tRNA_m1G_mtfrase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00088. trmD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMD_ANADF

Accession

Primary (citable) accession number: A7HBQ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 11, 2007
Last modified:	November 3, 2009
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)