Reviewed, UniProtKB/Swiss-Prot A7HB82 (ACEK_ANADF)

Names and origin

Protein names

Recommended name:
    Isocitrate dehydrogenase kinase/phosphatase
      Short name=IDH kinase/phosphatase
      Short name=IDHK/P
    EC=2.7.11.5
    EC=3.1.3.-

Gene names

Name:	aceK
Ordered Locus Names:	Anae109_1775

Organism

Anaeromyxobacter sp. (strain Fw109-5) [Complete proteome] [HAMAP]

Taxonomic identifier

404589 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Anaeromyxobacter

Protein attributes

Sequence length	575 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source of carbon. When bacteria are grown on glucose, IDH is fully active and unphosphorylated, but when grown on acetate or ethanol, the activity of IDH declines drastically concomitant with its phosphorylation By similarity.
Catalytic activity	ATP + [isocitrate dehydrogenase (NADP+)] = ADP + [isocitrate dehydrogenase (NADP+)] phosphate. HAMAP MF_00747
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the aceK family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	ATP-binding NADP Nucleotide-binding
Molecular function	Hydrolase Kinase Protein phosphatase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro glyoxylate cycle Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP [isocitrate dehydrogenase (NADP+)] kinase activity Inferred from electronic annotation. Source: HAMAP phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 575	575	Isocitrate dehydrogenase kinase/phosphatase HAMAP MF_00747		PRO_0000315261
Regions
	Nucleotide binding	316 – 322	7	ATP By similarity
Sites
	Active site	372	1	By similarity
	Binding site	337	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7HB82-1 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: 9CDC46B9822C96F7	FASTA	575	65,586
	10 20 30 40 50 60 MAEPNDVAAA GAAAIAASFE AYQEERARIT RRARERFEKR DWAAGQADAR ERLDLRDRLV 70 80 90 100 110 120 NACVGTVRAE LGGAAHDHGL WRAMKELYEA RVESRPDREI GQSFFNSVTR RVFVTVGVDP 130 140 150 160 170 180 AIEFLAADGP AVREGPVPVY ARYRREVSTE ALLRTVLRAC AFAAPYEDLE RDARIAAIEL 190 200 210 220 230 240 DSHLRELDDG QPIEALELVK AVFYRGKGAY LVGRLRRGRY TTPLVLALVH GERGVVLDAI 250 260 270 280 290 300 LFTQEDVSIV FSFTRSYFHV EVERPRELVA FLSTLLPLKR VSELYIALGF NKHGKTELYR 310 320 330 340 350 360 EIARHIAETG EAFVPARGDK GLVMSVFTLP GLDVVFKVIK DEFKPPKQTT RREVMDKYRH 370 380 390 400 410 420 VFRHDRAGRL VDAQEFEHLA FPADRFSPEV LRELSEECRG SIEIGRAEIS VKHLYAERRV 430 440 450 460 470 480 TPLNLFIREA DEWTARQAVL DFGRALKDLA ATNTFPGDLL LKNFGVTRHG RVIFYDYDEL 490 500 510 520 530 540 TRVTDCVFRD LPTPSGDDEE TSGEPWFYVG QDDVFPEELL PFLGLAGRLR EVFLQAHGDL 550 560 570 LTARYWRAIQ ERIREGEIVD IYPYREEQRL VHGYE

References

[1]

"Complete sequence of Anaeromyxobacter sp. Fw109-5." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Lykidis A., Fields M., Richardson P. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000769 Genomic DNA. Translation: ABS25978.1.
RefSeq	YP_001378962.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A7HB82.
Genome annotation databases
GeneID	5375321.
GenomeReviews	Gene locus Anae109_1775 in contig CP000769_GR.
KEGG	afw:Anae109_1775.
NMPDR	fig|404589.4.peg.1535.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	FYRDFLH.
Family and domain databases
HAMAP	MF_00747. [Tree]
InterPro	IPR010452. Isocitrate_DH_AceK. [Graphical view]
Pfam	PF06315. AceK. 1 hit. [Graphical view]
PIRSF	PIRSF000719. AceK. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ACEK_ANADF

Accession

Primary (citable) accession number: A7HB82

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 11, 2007
Last modified:	November 3, 2009
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)