Reviewed, UniProtKB/Swiss-Prot A7HA60 (PUR9_ANADF)

Names and origin

Protein names

Recommended name:
    Bifunctional purine biosynthesis protein purH
Including the following 2 domains:
    1- Recommended name:
            Phosphoribosylaminoimidazolecarboxamide formyltransferase
              EC=2.1.2.3
        Alternative name(s):
            AICAR transformylase
    2- Recommended name:
            IMP cyclohydrolase
              EC=3.5.4.10
        Alternative name(s):
            Inosinicase
            IMP synthetase
            ATIC

Gene names

Name:	purH
Ordered Locus Names:	Anae109_1399

Organism

Anaeromyxobacter sp. (strain Fw109-5) [Complete proteome] [HAMAP]

Taxonomic identifier

404589 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Anaeromyxobacter

Protein attributes

Sequence length	524 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP MF_00139 IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP MF_00139
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1. HAMAP MF_00139 Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.
Domain	The IMP cyclohydrolase activity resides in the N-terminal region By similarity. HAMAP MF_00139
Sequence similarities	Belongs to the purH family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Molecular function	Hydrolase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	IMP cyclohydrolase activity Inferred from electronic annotation. Source: HAMAP phosphoribosylaminoimidazolecarboxamide formyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 524	524	Bifunctional purine biosynthesis protein purH HAMAP MF_00139		PRO_1000018835

Sequences

	Sequence		Length	Mass (Da)
	A7HA60-1 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: B5BD1A674B490F9C	FASTA	524	56,191
	10 20 30 40 50 60 MVRRALVSVS DKTGLVPFAK RLAALGVEIL STGGTQRALA DAGVPVVSVG DYTQAPEILA 70 80 90 100 110 120 GRVKTLHPRV HGGILYRRGL ASDEADVKAR DIPPIDLVVV NLYPFREAVA AGKPFWDCVE 130 140 150 160 170 180 EIDIGGPTMV RSAAKNAAHV GVVVDPADYE RVAAELEASR ALSDQTRFEL MKKAFAHTAA 190 200 210 220 230 240 YDAAISEFLT ARESTDAQAK RFPATLAAVY SKAGDLRYGE NPHQAGAFYR AGREPDEPTV 250 260 270 280 290 300 AFAKVLQGKE LSYNNLLDLE AALAAVKEHD EVACVVIKHN TPCGVSLGKT PAEAFARARA 310 320 330 340 350 360 CDPVSAFGGI VALNRPVDAA AAKELTDLFL ECVIAPGYDE AARAALGAKK NLRLLEAPRL 370 380 390 400 410 420 AEPRTSWTRR PEELRELRSI PGGLLVMDRD LGAIRRDDCK VMTKRAPTDA EWEDLLFAWK 430 440 450 460 470 480 VVKHVKSNAI VFAKEKRTVG IGGGQTSRVE SVKTAVMKAQ LELVGSTVGS DAFFPFKDGV 490 500 510 520 EEIIKAGATA IIQPGGSVRD PEVIEAADAA NVAMVATGMR HFRH

References

[1]

"Complete sequence of Anaeromyxobacter sp. Fw109-5." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Lykidis A., Fields M., Richardson P. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000769 Genomic DNA. Translation: ABS25606.1.
RefSeq	YP_001378590.1.
3D structure databases
SMR	A7HA60. Positions 2-524.
ModBase	Search...
Protein-protein interaction databases
STRING	A7HA60.
Genome annotation databases
GeneID	5374120.
GenomeReviews	Gene locus Anae109_1399 in contig CP000769_GR.
KEGG	afw:Anae109_1399.
NMPDR	fig|404589.4.peg.1214.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0138.
HOGENOM	HBG498048.
OMA	VVKHVKS.
Family and domain databases
HAMAP	MF_00139. PurH. [Tree]
InterPro	IPR002695. AICARFT_IMPCHas. IPR013982. AICARFT_IMPCHase_bienz. IPR011607. MGS. [Graphical view]
PANTHER	PTHR11692. AICARFT_IMPCHas. 1 hit.
Pfam	PF01808. AICARFT_IMPCHas. 1 hit. PF02142. MGS. 1 hit. [Graphical view]
PIRSF	PIRSF000414. AICARFT_IMPCHas. 1 hit.
SMART	SM00798. AICARFT_IMPCHas. 1 hit. SM00851. MGS. 1 hit. [Graphical view]
TIGRFAMs	TIGR00355. purH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR9_ANADF

Accession

Primary (citable) accession number: A7HA60

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 11, 2007
Last modified:	February 9, 2010
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)