A7H9R0 (A7H9R0_ANADF) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase HAMAP MF_00193 RuleBase RU003812
EC=6.3.1.5 HAMAP MF_00193 RuleBase RU003812

Gene names

Name:	nadE HAMAP MF_00193
Ordered Locus Names:	Anae109_1248

Organism

Anaeromyxobacter sp. (strain Fw109-5) [Complete proteome] [HAMAP]

Taxonomic identifier

404589 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Anaeromyxobacter

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193 RuleBase RU003812 SAAS SAAS003694
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193 RuleBase RU004252 SAAS SAAS003694
Sequence similarities	Belongs to the NAD synthetase family. HAMAP MF_00193 RuleBase RU003811

Ontologies

Keywords
Ligand	ATP-binding HAMAP MF_00193 SAAS SAAS003694 RuleBase RU003811 NAD HAMAP MF_00193 SAAS SAAS003694 RuleBase RU003811 Nucleotide-binding
Molecular function	Ligase SAAS SAAS003694 HAMAP MF_00193 RuleBase RU003811 EMBL ABS25456.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	38 – 45	8	ATP By similarity HAMAP MF_00193
Sites
	Active site	40	1	By similarity HAMAP MF_00193

Sequences

	Sequence		Length	Mass (Da)
	A7H9R0 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: AFB5FFBA7B2A6A28	FASTA	324	35,698
	10 20 30 40 50 60 MFTRDVLRID AARAAEGIEQ AIRDQVLGTL RRRGAVVGMS GGIDSSVVAT LCTRALGADR 70 80 90 100 110 120 VLGLLMPERD SSSDALRLGR SLADHLGIRH LVEDVGPALA GLGCYERQLE AIRSVVPEYG 130 140 150 160 170 180 EGWRCKLTLP SLLAGDRLNV TLLTVADPLG RERSSRMSAA AYLQLVAATN FKQRARKMME 190 200 210 220 230 240 YYHADRLNFA VAGTPNRLEY DQGFFVKQGD GAADFKPIAH LYKTQVYALA EHLGVPGEIR 250 260 270 280 290 300 ARPPTTDTFS LSQTQEEFFF ALPYREMDLC LWAHDHEVSA AQVGAALGLT AEQVERVYRD 310 320 IEAKRRASRY LHEAPLLVTE LPGS

References

[1]

"Complete sequence of Anaeromyxobacter sp. Fw109-5." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Lykidis A., Fields M., Richardson P. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Fw109-5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000769 Genomic DNA. Translation: ABS25456.1.
RefSeq	YP_001378440.1. NC_009675.1.
3D structure databases
ProteinModelPortal	A7H9R0.
ModBase	Search...
Protein-protein interaction databases
STRING	A7H9R0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5375926.
GenomeReviews	Gene locus Anae109_1248 in contig CP000769_GR.
KEGG	afw:Anae109_1248.
NMPDR	fig|404589.4.peg.1092.
PATRIC	20927651. VBIAnaSp113478_1307.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0171.
HOGENOM	HBG351567.
OMA	AHLYKSQ.
ProtClustDB	PRK00876.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K01916.
Pfam	PF02540. NAD_synthase. 2 hits. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A7H9R0_ANADF

Accession

Primary (citable) accession number: A7H9R0

Entry history

Integrated into UniProtKB/TrEMBL:	September 11, 2007
Last sequence update:	September 11, 2007
Last modified:	December 14, 2011
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)