A7H9F9 (DUT_ANADF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolase
Short name=dUTPase
EC=3.6.1.23

Alternative name(s):
dUTP pyrophosphatase

Gene names

Name:	dut
Ordered Locus Names:	Anae109_1147

Organism

Anaeromyxobacter sp. (strain Fw109-5) [Complete proteome] [HAMAP]

Taxonomic identifier

404589 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Anaeromyxobacter

Protein attributes

Sequence length	147 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA By similarity. HAMAP MF_00116
Catalytic activity	dUTP + H2O = dUMP + diphosphate. HAMAP MF_00116
Cofactor	Magnesium By similarity. HAMAP MF_00116
Pathway	Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2. HAMAP MF_00116
Sequence similarities	Belongs to the dUTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	dUTP metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	dUTP diphosphatase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 147	147	Deoxyuridine 5'-triphosphate nucleotidohydrolase HAMAP MF_00116		PRO_1000015442
Regions
	Region	67 – 69	3	Substrate binding By similarity
	Region	84 – 86	3	Substrate binding By similarity
Sites
	Binding site	80	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7H9F9 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: CE9351AD7BAF05CA	FASTA	147	15,325
	10 20 30 40 50 60 MPVTVKVRRV GQRGPPLELP RYETDGAAGL DLRADEPVTL APGERRLVPT GLALEIPPGH 70 80 90 100 110 120 EGQVRPRSGL AVRHGVGMVN APGTIDSDYR GEVGVVLVNH GQEPVTFARG DRIAQLVIGP 130 140 VVRAELALVE DLASSGRGGG GFGSTGR

References

[1]

"Complete sequence of Anaeromyxobacter sp. Fw109-5." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Lykidis A., Fields M., Richardson P. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Fw109-5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000769 Genomic DNA. Translation: ABS25355.1.
RefSeq	YP_001378339.1. NC_009675.1.
3D structure databases
ProteinModelPortal	A7H9F9.
ModBase	Search...
Protein-protein interaction databases
STRING	A7H9F9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5376991.
GenomeReviews	Gene locus Anae109_1147 in contig CP000769_GR.
KEGG	afw:Anae109_1147.
NMPDR	fig|404589.4.peg.1002.
PATRIC	20927439. VBIAnaSp113478_1202.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0756.
HOGENOM	HBG436079.
OMA	KIAQMVI.
ProtClustDB	PRK00601.
Family and domain databases
HAMAP	MF_00116. dUTPase_bact. [Tree]
InterPro	IPR008180. dUTP_pyroPase. IPR008181. dUTP_pyroPase_sf. [Graphical view]
KO	K01520.
PANTHER	PTHR11241. PTHR11241. 1 hit.
Pfam	PF00692. dUTPase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00576. Dut. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DUT_ANADF

Accession

Primary (citable) accession number: A7H9F9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 11, 2007
Last modified:	January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program